You did amazing job here. Ohh My God!!! Thank you so much for this explanation of oligonucleotides synthesis! It was really helpful for me and my learning process at my job! God bless your knowledge
This is the best video I have seen about the subject. It is really well explained and easy to understand and follow. What I like most about this lecture is that I feel engaged. My teacher tried to explain this in a video lecture, but I just could not follow.
Sorry, Robert Bruce Merrifield (July 15, 1921 - May 14, 2006) was an American biochemist who won the Nobel Prize in Chemistry in 1984 for the invention of solid phase PEPTIDE synthesis. The phosphoramidite method for oligonucleotides synthesis has been first published by Marvin Caruthers in 1979-80 !!!
Very beautiful video ma'am ❤️understood the concept well ,I was a bit confused when I came to the capping step like why is it done and you solved my doubt 🙏
I have a question.. When you cap the first nucleotide that didn't react in order for the third nucleotide not to bind to it, how do you make it do the second nucleotide isn't capped as well? Thanks for the video! it's very helpful :)
I believe it's because the 1st nucleotide under went detritylation and the DMT molecule was removed from the nucleotide and replaced by hydrogen. Since you added the second nucleotide AFTER detritylation, it would still have the DMT group attached, and therefore be protected during capping. So during capping the remnants of nucleotide 1 would be capped, and nucleotide 2 would be unaffected. Once you repeat the process and perform detritylation again, the DMT will be replaced with H on the 2nd nucleotide, the 3rd nucleotide will attach to the 2nd, and then the remnants of the 2nd nucleotide will be capped. Hope that's helpful!
This video is very helpful for all biotechnology student . Thank you mam...
It's a Complete video , no corners are untouched here and with very good explanation
Thankyou Mam 🙏
You did amazing job here. Ohh My God!!! Thank you so much for this explanation of oligonucleotides synthesis! It was really helpful for me and my learning process at my job! God bless your knowledge
This is the best video I have seen about the subject. It is really well explained and easy to understand and follow. What I like most about this lecture is that I feel engaged. My teacher tried to explain this in a video lecture, but I just could not follow.
Wow, thank you!
@@IntrovertGeekmam plz ans my question..why we use only dmt for 5' protection over other hydroxy
protecting groups.. plz ans mam
I must say I've never seen such detailed explanation about this topic ...
Thanku so much this is really very helpful...🙏😊😊
Thank you so much, been looking for a straight forward but detailed video to get the idea and chemistry of oligs. Thanks again!
Ty!
Excellent analysis 👍
Weldone
Thank you!!! I needed something like this to show our team 🙏🏻
You're so welcome!😊
Nice Explanation, Thank You!
Excellent way of teaching.
very nice explanation...
Good explanation ✅
You just earned a subscriber :) totally radical explanation thanks!
Awesome, thank you!
Thank you mam for this video. Really easy to understand.
very well explained.
This is gold! Thank you so mucho!
Most helpful 😍
Please tell me where I can find photographic evidence of the DNA molecule.
Thank you for explaining. It's well-written and detailed.
Glad it was helpful!
So informative, well explained really much helpful for my exams ,thankyou 👍 😊
This video was zo helpfull!!! Thank you soo much!!!
Good job making a video out of a high-quality wikipedia article. You can be proud of yourself
This is absolutely amazing. Great explanation, thanks.
Which disease, will it help?
Thank u 😊Its very helpful for my seminar😄
Thanks for the great video! so well explained.
Glad it was helpful!
Sorry, Robert Bruce Merrifield (July 15, 1921 - May 14, 2006) was an American biochemist who won the Nobel Prize in Chemistry in 1984 for the invention of solid phase PEPTIDE synthesis.
The phosphoramidite method for oligonucleotides synthesis has been first published by Marvin Caruthers in 1979-80 !!!
Thank you so much, very helpful video
Glad it was helpful!😊
Very useful
Perfect, now for a wet lab?
Very beautiful video ma'am ❤️understood the concept well ,I was a bit confused when I came to the capping step like why is it done and you solved my doubt 🙏
Glad to hear!
So well explained. Thank you very much.
It would be helpful if you could make a video on protecting groups..
Thank you very much!! It‘s easy to understand.
Your welcome, Glad to hear that😊!
We cap to protect also from oxydation
Very helpful
🔥🔥
This is great. Thank you so much .
You're very welcome!
Thankyou ❤😊❤
Superb but plss give d abbreviation of DMT
I have a question..
When you cap the first nucleotide that didn't react in order for the third nucleotide not to bind to it, how do you make it do the second nucleotide isn't capped as well?
Thanks for the video! it's very helpful :)
I believe it's because the 1st nucleotide under went detritylation and the DMT molecule was removed from the nucleotide and replaced by hydrogen. Since you added the second nucleotide AFTER detritylation, it would still have the DMT group attached, and therefore be protected during capping. So during capping the remnants of nucleotide 1 would be capped, and nucleotide 2 would be unaffected. Once you repeat the process and perform detritylation again, the DMT will be replaced with H on the 2nd nucleotide, the 3rd nucleotide will attach to the 2nd, and then the remnants of the 2nd nucleotide will be capped. Hope that's helpful!
Wowwwwwww ❤️❤️❤️
Glad you liked it!🤗
@@IntrovertGeek plz help me what Different methods used in the Analysis of DNA, including synthesis and sequencing
thanks a lot, very informative
Glad it was helpful!
Thank u🤩
TCA - trichloroacetic acid
Thank you!!
You're welcome!
This video was zo helpfull!!! Thank you soo much!!!
Thank you !!!!
You're welcome!