Вставка
- Опубліковано 15 гру 2020
- There are several assays to #quantify protein in a solution 🧪. One of them is the #Bradford assay which is a #spectroscopic analytical method developed by Marion M. Bradford.
This assay is a #colorimetric assay based on an #absorbance shift of a #dye called #Coomassie Brilliant Blue 💙. This dye exists in an anionic (blue), neutral (green) and cationic (red) state. The solution is brown if no #protein is present. The proteins interact with the dye which upon interaction goes into its blue state. The asssay is dependent on the amino acid composition of the protein since it's dependent on the #chemistry of the amino acid side chains.
The absorbance at 595 nm (blue state) can be measured with a #Tecan plate reader 💙. The absorbance increase is proportional to the amount of blue state (bound dye) and this to the amount of protein in the sample 🕵️♀️.
Have a nice Thursday 🤗
#NCCR #NCCRmse #Lab #Science #scicomm #wissenschaftskommunikation - Наука та технологія
