Radioimmunoassay technique (RIA)

This lecture explains about radioimmunoassay techniques or RIA. Radioimmunoassay (RIA) test is a very important in vitro assay method used to measure concentrations of antigens (for illustration, hormone levels in blood) through use of antibodies. As such, it may be visible because the inverse of a radiobinding assay, which quantifies an antibody via use of corresponding antigens.
Watch this video lecture to understand the mechanism of Radioimmunoassay also known as RIA.
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Thank you sir. I work in a Regulatory Science division. I needed to understand RIAs and now I do.

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[@abdullahmarwat2714]

according to RIA when radiolabeled Ag combine with Ab ,the reaction will occur and it gives a signal like color graph or whatever so, but only free labeled Ag give no result. So concentration of Ag (in patient sample) is inversly propotional to the radioactivity.

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But what we studied was there are blocking agent which gets displaced & also the supernatent is discarded rather than detected for radioactivity!! Also conc. is inversely proportional to radioactivity. I think i m more confused now!!

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[@kapamed3041]

the video is quite helpful but is it possible to carry out this technique in the reverse manner i.e. looking for antibody when antigen is known? like in the wells will be antigens radioactively labelled and then we introduce patients serum to detect if antibodies to that antigen are present?

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