It's an enticing brief about aptamer project.. Would you please give some practical experiences via your channel for this topic?... It is very comprehensive and excellent description.. Thank you mam
You need to perform SELEX and characterize the selected aptamer based sequence characterization using In silico approach...If you don't wanna perform wet lab SELEX, in that case you use computational approach and ML algorithm based approach
mam ; In India where can we opt. for SELEX technology; i mean in which institutes. 2nd - what are the techniques of validating aptamer binding to our molecule. Kindly help
There are limited research lab and institutes working on Aptamer technology in India, you may opt for JNU, IITs, IMTECH and NII for such research... It's a principal based technology so the techniques and protocols requirements varies depending on individual projects needs .. You may approach us for training and consultation regarding aptamer research.. Send your CV to jbala1444@gmail.com and info@molelixirinformatics.com and book your consultation session
Very informattive video maam I have worked 9 month on this so i am exciting to know more about it
Thankyou so much..Yes I will be soon uploading more about this area especially SELEX technical details.
Very informative video ma'am
Thankyou
It's an enticing brief about aptamer project.. Would you please give some practical experiences via your channel for this topic?... It is very comprehensive and excellent description.. Thank you mam
Thankyou...Sure ...I will upload soon.
Thank you for these good information, I have one question. How can I choose the exact sequence to design the aptamer?
You need to perform SELEX and characterize the selected aptamer based sequence characterization using In silico approach...If you don't wanna perform wet lab SELEX, in that case you use computational approach and ML algorithm based approach
mam, please suggest me the Insilco soft wares by which we can design a DNA aptamer
You may try E2EDNA, a simulation protocol or RAPTranker
@@DrJyotiBala thank you so much, I will try with your recommendations
mam ; In India where can we opt. for SELEX technology; i mean in which institutes.
2nd - what are the techniques of validating aptamer binding to our molecule.
Kindly help
There are limited research lab and institutes working on Aptamer technology in India, you may opt for JNU, IITs, IMTECH and NII for such research...
It's a principal based technology so the techniques and protocols requirements varies depending on individual projects needs ..
You may approach us for training and consultation regarding aptamer research..
Send your CV to jbala1444@gmail.com and info@molelixirinformatics.com and book your consultation session
Mam, i have to design aptamer for bacterial species for nanosensing purpose
Kindly send us your requirement either service or aptamer training at info@molelixirinformatics.com or jbala1444@gmail.com
Ma'am how to make dna library?
You may either order synthetic DNA library or can do asymmetric PCR to generate library
How we select particular sequence? Which is against my Target of intrest
Dr, is it possible to generate aptamers through in silico methods?
Yes, it's possible to generate Aptamers with the In Silico approach......But functional characterization requires wet lab experiment
@@DrJyotiBala would you mind make a video on how to generate aptamers with in silico approach, it will be very helpful ma'am. Thank you 😊
@@khavieureka3627 Sure, I shall be uploading that soon
@@DrJyotiBala Thank you so much Dr.
May God bless you always:)