Hello I found your video very helpful but I have just a few questions. The first one is, is agar in the Petri dishes harmful and what are the disks you used to add in the cleaning liquids. Thank you!
Thanks. I want to check bacteria germs in some kitchens, bathrooms in my Co-OP building and a neighborhood bar across the street. I don't have an "incubator" but you said it was more about a warm ~ 90 F dry place. That should be something I could do, how about an oven? Another question, can I sterilize the Q Tip with just bowling water, then putting the Q tip to dry then in to a plastic bag? Thanks Jaye Left behind in Chicago
I love your methods! The UV light thing got me thinking. Could I put an Agar petri dish in a UVC chamber (I have one for erasable microchips) with the lid on to kill anything that's in the dish before using it? It might be overkill but I too do not have a big budget and would like to do my best to act clean.
So here where I live where 90 fahrenheit is like ambient temperature at night and 100 fahrenheit is ambient temperature at day, is a regular shelf an incubator?
I have a question. Instead of using a cleaner for your examples, is it possible to use an antibiotic or some kind of solution instead to see if bacteria grows near it?
How much agar do I need for a petri dish do I need to fill it full of just enough to fill up blank spaces I keep failing so I need some advice and helps
If it is nutrient agar, then no, if it is just pure agar, then I add a soup bouillon cube. I'm going to experiment with better smelling things besides a bouillon cube though.
@@crystelmeursecret It gives the bacteria something to eat. If you don't give them something to eat they will not grow. If have made that mistake in the past and wondered why nothing was growing.
Sir I want to grow a particular type of gut bacteria. But I am new to lab - experiment thing. Can u suggest me how to separate a particular bacteria and grow it in petri dish without interference of other bacteria.
I know I'm late to this video, but I have a question: in this video and your video on making the petri dishes, you kept turning the dishes upside down. Why do you do that?
I have a question. I have a task to grow salmonella at home so do you think it would be good if I took bacterias from raw chicken? Or do you have better idea?
If there is a small number of colonies I just count them, or if there are lots of little ones, I would put a grid behind the dish and count a couple squares and then and extrapolate.
Most people will either put them into an autoclave/pressure cooker which can be really smelly or they will put them in a bag that is labeled as biohazard and disposed of properly.
Has anybody thought about finding somebody who is infected with covid-19 or the Omicron virus and swabbing their mouth to see what grows in a petri dish? Or if they cough up phlegm test that in a petri dish
I bet you never expected growing things on agar would have such a broad range of interested parties. Thanks for your content.
Thanks for watching and enjoying!
@@brightbiology9632 Do you know how to grow good smelling bacteria 🦠? If so can you do a video on it?
Thank you I can’t wait to grow my friends :>
Haha! Nice!
awww
😂😂
I’m going to try doing this but I’m not gonna let it stay alive for ever
enthusiastically liked this video!!
great job!
Thank you so much!
Hello I found your video very helpful but I have just a few questions. The first one is, is agar in the Petri dishes harmful and what are the disks you used to add in the cleaning liquids. Thank you!
Thanks.
I want to check bacteria germs in some kitchens, bathrooms in my Co-OP building and a neighborhood bar across the street.
I don't have an "incubator" but you said it was more about a warm ~ 90 F dry place. That should be something I could do, how about an oven?
Another question, can I sterilize the Q Tip with just bowling water, then putting the Q tip to dry then in to a plastic bag?
Thanks Jaye
Left behind in Chicago
I love your methods!
The UV light thing got me thinking.
Could I put an Agar petri dish in a UVC chamber (I have one for erasable microchips) with the lid on to kill anything that's in the dish before using it?
It might be overkill but I too do not have a big budget and would like to do my best to act clean.
I'm just wondering, what kind of bacteria did you get when you dug a soil? is it bacillus subtilis?
"I'm going to swab for 10 seconds.."
So the 5 second rule is TRUE!!!!!
i live in Guernsey channel islands where can i get these dishes from the ones with the red stuff in side
So here where I live where 90 fahrenheit is like ambient temperature at night and 100 fahrenheit is ambient temperature at day, is a regular shelf an incubator?
I would definitely think so. You just should keep them in a container so they don't dry out.
I have a question. Instead of using a cleaner for your examples, is it possible to use an antibiotic or some kind of solution instead to see if bacteria grows near it?
I have used penicillin and other types of antibiotic disks and got great results with that.
How much agar do I need for a petri dish do I need to fill it full of just enough to fill up blank spaces I keep failing so I need some advice and helps
Thanks for this video! I want to do something like this for a kid’s camp but have limited time. Did you have growth within 1-2 days?
Yes, if it has the correct growing environment.
Hi, sir. I find your video really beginner-friendly. I just want to know if you add something on the agar?
If it is nutrient agar, then no, if it is just pure agar, then I add a soup bouillon cube. I'm going to experiment with better smelling things besides a bouillon cube though.
@@brightbiology9632 Thank you sir🥰
@@brightbiology9632 hi sir, can I ask what's the reason why we need to add bouillon cube?thank you!
@@crystelmeursecret It gives the bacteria something to eat. If you don't give them something to eat they will not grow. If have made that mistake in the past and wondered why nothing was growing.
Great video
Do you need to have a BSL-1 level lab for this kind of experiment?
I have an incubator but I don’t know what temperature it should be???
Sir I want to grow a particular type of gut bacteria. But I am new to lab - experiment thing. Can u suggest me how to separate a particular bacteria and grow it in petri dish without interference of other bacteria.
I know I'm late to this video, but I have a question: in this video and your video on making the petri dishes, you kept turning the dishes upside down. Why do you do that?
Moisture management and keeps contaminants from landing on the media.
Why do the dishes look like there is condensations in them?
I have a question. I have a task to grow salmonella at home so do you think it would be good if I took bacterias from raw chicken? Or do you have better idea?
Just take samples from your daily techs like computer,phone etc
What item do you dunk in the clorox to put into the petri dish?
Just a cut piece of cheap paper towel from school.
3:53 the burner sounds like a rocket engine
how do you quantitate the bacteria on the dish?
If there is a small number of colonies I just count them, or if there are lots of little ones, I would put a grid behind the dish and count a couple squares and then and extrapolate.
How can you tell you are growing bacteria and not mold or fungi?
if the area you've placed the infected dish it should only get bacteria
Sir how do i dispose my petri dishes properly? Can i just burn it all out or not? Thank you!
Most people will either put them into an autoclave/pressure cooker which can be really smelly or they will put them in a bag that is labeled as biohazard and disposed of properly.
Can i use gelatin powder as agar agar
I think it would work, but never tried. Just make sure you make it thick enough.
How to grow one type of bacteria in petri dish?
There are many ways of accomplishing this. Here is just one example:
ua-cam.com/video/DbRzNgoIF7s/v-deo.html
Here is another video about how to get one type of bacteria. ua-cam.com/video/EP5sr7f1qxs/v-deo.html
can you use food agar to grow bacteria?
Definitely.
Do you have Sharon Petrie dish?
Sorry auto correct. Do you have agar in the Petrie dish
yes, without some agar you are not going to see any growth
Better yet, how to grow mycelium in a petri dish.
Hi new sub
Bro looks like senator armstrong
I've been told I look more like Megamind.
Has anybody thought about finding somebody who is infected with covid-19 or the Omicron virus and swabbing their mouth to see what grows in a petri dish? Or if they cough up phlegm test that in a petri dish
Remember that a virus is different than bacteria. The things that grow in most petri dishes are bacteria and fungi.
E
Why does my agar melt,and what can I do?
You probably didn't use enough agar powder or didn't heat up the mixture enough.