Understanding of

The MTT assay is a colorimetric assay for assessing cell metabolic activity. Tetrazolium dye assays can also be used to measure cytotoxicity (loss of viable cells) or cytostatic activity (shift from proliferation to quiescence) of potential medicinal agents and toxic materials. (dimethylthiazol-diphenyltetrazolium bromide)

[@allpharmacyupdatesforpharm6422]

Explained in very simple language. Very informative video.

[@aondohembanege9551]

Hi, thanks for the video. Please, I have a question: I will like to ask a question about cell treatment experiment with a test drug for 72 h. In such an experiment, do I have to add my drug once and incubate for 72h then check the cell viability or I have to remove the drug solution and replace with the same drug and concentration after each 24h to avoid wrong readings due to degraded drug in wells or evaporation? Please any other person reading the comments can also reply me if you have the correct answer to my question. Thank you

[@VamsikrishnaIthadi]

Tq soo much sir