Steps in Recombinant DNA Technology or rDNA technology | Biotechnology

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  • Опубліковано 5 вер 2024

КОМЕНТАРІ • 374

  • @biologyexams4u
    @biologyexams4u  Рік тому +3

    Hi Dear Friends, Hope this video helped.
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  • @biologyexams4u
    @biologyexams4u  4 роки тому +26

    Dear All
    This is one of the Most watched video on rDNA technology on UA-cam with your support.
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    ua-cam.com/users/biologyexams4u
    Detailed explanation of steps in recombinant DNA technology explained with the example of insulin synthesis in bacterium.
    Your support is awesome..... Thank you so much :) @biologyexams4u
    Our channel has crossed 50K subscribers with Almightys grace and your support
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  • @biologyexams4u
    @biologyexams4u  8 років тому +30

    Biologyexams4u's first video crossing 100000 views. Heartfelt thanks to all for supporting this venture. This video was simply an attempt to explain my understanding in rDNA technology and turned out to be our best video only because of your appreciation and support.....Still I love to be known as a student of life science than a teacher of life science. This wouldn't have been possible without your encouragement......still deep in love with biology....Once again thanking you so much...........

    • @abhithakur6351
      @abhithakur6351 6 років тому

      biologyexams4u

    • @taylorzane6224
      @taylorzane6224 6 років тому

      biologyexams4u I was searching a appropriate video which can help me to understand my NCERT syllabus but all I've got is stuff but this video is amazing I mean all of my doubts has gone now

  • @noortahir7586
    @noortahir7586 3 роки тому +8

    Step 1: Identification and isolation of Gene of interest.
    Step 2: Insertion of this isolated gene in a suitable vector.
    Step 3: Introduction of this Vector into a suitable host.
    Step 4: Selection of the transformed host cell.
    Step 5: Multiplication or expression of the introduced gene in the host.
    Your welcome

  • @sweetysmart5089
    @sweetysmart5089 9 років тому +30

    I thnk this guy z indian... bt good explainatn wid explainatory slides... thnxx sir fr d video..

  • @zophanem8062
    @zophanem8062 8 років тому +5

    im currently in a medical school year 1 learning biochemistry. i really wish the teaching method is this way. it is much easier to understand from this video. thanks alot :)

  • @wrothofthepyros
    @wrothofthepyros 7 років тому +1

    It helps immensely to eatch over these videos before exams. Thanks so much for making them!

  • @abcarodefg
    @abcarodefg 6 років тому

    THANK YOU SO MUCH I have been reading and researching papers after papers trying to find the steps and they are all scattered and then there is this one that just combines everything together helping me put everything together and in order.

  • @indirac.4010
    @indirac.4010 9 років тому +3

    I absolutely love this video!
    Its shows the fundamental steps in easy to understand points and visual for insulin production!
    thank you!

  • @k.s.sivakami2144
    @k.s.sivakami2144 3 роки тому +2

    Such an understandable video I have ever seen... Thank you sir for the clear cut explanation about the RDT..

  • @oluwajefunmi
    @oluwajefunmi 2 місяці тому

    The most useful video I have still seen on rDNA. Very structured. 👍

  • @678994A
    @678994A 9 років тому +3

    Amazing explanation. It was very clear and easy to follow.

  • @supratiksaha2001
    @supratiksaha2001 5 років тому +1

    Thank you for your clear cut vidoe. I understood this complicated but interesting topic from your video easily.Thank you a lot.😊

  • @umarsahil2999
    @umarsahil2999 6 років тому

    thats really a great and the best method of explaining the lecture.... i was having problems in this topic now i am having nothing difficult....... thanks sir!!!!!!

  • @MelissaM96
    @MelissaM96 8 років тому

    Best explanation about rdna ever.. even my lecturer used this in our class, thank u soo much:)

  • @hasithbuddhima4803
    @hasithbuddhima4803 4 роки тому +2

    thank Mister. your prasentation helped me a lot to understand r DNa tech clearly. nice work

    • @biologyexams4u
      @biologyexams4u  4 роки тому

      Welcome Hasith...to @biologyexams4u. Really glad to know the video helped. TNQ for the support :)

  • @gabrielkateta7162
    @gabrielkateta7162 7 років тому

    Good explanation for the main steps involving rDNA technology. I like it !!!

  • @manishathakur8647
    @manishathakur8647 5 років тому +2

    Sir your lectures are of a great use for us .really you are amazing.

  • @diyakasaragod1152
    @diyakasaragod1152 7 років тому

    thank you so much sir. this video really helped me understand recombinant dna tech better! way to go!!

  • @bengisu7643
    @bengisu7643 6 років тому

    THANK YOU I HAVE QUIZ TOMORROW AND THIS VIDEO SAVED MY LIFE

  • @waseemmaqbool822
    @waseemmaqbool822 7 років тому +1

    Why don't all the people who watch ua videos subscribe you.......Your videos are simply awesome...Appreciated ..Gr8 EFFORTS

  • @saanviandsanndhishirakol452
    @saanviandsanndhishirakol452 Рік тому +3

    Plz make more videos on rDNA technology sir. Ur all videos are very clear n helps to build more knowledge.

    • @biologyexams4u
      @biologyexams4u  Рік тому +1

      Really glad that the videos helped
      Playlist of rDNA technology videos: ua-cam.com/play/PLpKNQ2U3np9jx0Gjl66YK6O3nel6A93zr.html
      Stay blessed. Thank you

    • @saanviandsanndhishirakol452
      @saanviandsanndhishirakol452 Рік тому

      @@biologyexams4u tnq for providing the link sir.... U rock!!!!!!!!!!

  • @gokulgirish37
    @gokulgirish37 8 років тому

    I thank the whole team heartily. Tysm

  • @rafikhan5479
    @rafikhan5479 7 років тому

    Really amazing!
    Finger crossed for your fore assignments!

  • @likhitareddy4033
    @likhitareddy4033 7 років тому

    really very informative.got a clarity and perfection.thankyou very much

  • @ibadatglory4371
    @ibadatglory4371 9 років тому +12

    wow .....!...i must say this video is highly useful . its explained in a very alluring and easy way ....thanks.....:)

  • @anandrahangdale5214
    @anandrahangdale5214 9 років тому

    This video really helped me ! 2 questions were asked from this in MH-PMT this year

  • @arvinrkr
    @arvinrkr 9 років тому +2

    amazing example of mallu accent....mallus are the best!!!

  • @iansong8137
    @iansong8137 9 років тому +1

    Thanks for such a wonderful video. It helped me greatly for by biology test.

    • @biologyexams4u
      @biologyexams4u  9 років тому

      Ian Song
      Welcome and very glad to know that it helped. Thanks for the for visit and motivating comment

    • @iansong8137
      @iansong8137 9 років тому

      biologyexams4u By the way I got top in my class for writing an essay on this topic after watching your video...

  • @GaneshChaitanya
    @GaneshChaitanya 9 років тому

    Really wonderful video n narration....very useful for anyone in medical field.

  • @vishujadhav6930
    @vishujadhav6930 5 років тому +4

    Tommorow is my biology paper and you saved me. Thank you very much.😀😀

    • @biologyexams4u
      @biologyexams4u  5 років тому

      Hi Vishnu
      Really happy that the video helped.
      thank you so much for the support:)

  • @beartrader8634
    @beartrader8634 8 років тому

    Help you man so much! Thank you!Good luck !

  • @cheezecakee1
    @cheezecakee1 10 років тому

    awsome thank u lifesaving well explained, good speed of talking too :)

    • @biologyexams4u
      @biologyexams4u  10 років тому

      Thank you for the visit and the comment... cheezecakee1

  • @palashariq7765
    @palashariq7765 3 роки тому +2

    Nice explanation!
    Thank u so much sir

  • @jasmeetsandhu4831
    @jasmeetsandhu4831 5 років тому +1

    Crystal clear explaination ,Thanks a lot sir

  • @subhashinisivagobi9995
    @subhashinisivagobi9995 5 років тому +1

    i have an exam tomorrow and you just saved me. Thank you.

    • @biologyexams4u
      @biologyexams4u  5 років тому

      Really Happy to know that it helped. Best Wishes for the Exam

  • @devipriya6167
    @devipriya6167 5 років тому +1

    Thanks sir very useful points r DNA technology

  • @khadimhussainsapu601
    @khadimhussainsapu601 7 років тому +2

    thnx alot dear sir.... u r really outstanding
    i wish u were our college professor....!!
    lot's oflove u dear sir

  • @amritagangdeb4317
    @amritagangdeb4317 2 роки тому +2

    I find difficulties to understand but after watching ur video all my doubts have gone 😀

  • @meowismeify
    @meowismeify 9 років тому

    Thank you for your videos!! They are very helpful!!

  • @sridevip441
    @sridevip441 4 роки тому +1

    Thank you. Easy & understandable

  • @Oldmansland1
    @Oldmansland1 9 років тому

    Good work. Thanks a lot!!

  • @sonammeena8674
    @sonammeena8674 6 років тому

    nice video. it covers everything in a simple and interesting way

  • @abhinayamadhavan2794
    @abhinayamadhavan2794 7 років тому

    difficult concept with simple teaching ....thank you

  • @elkanahhaon1790
    @elkanahhaon1790 7 років тому

    Very helpful overview, thanks!

  • @vjshaandilya3790
    @vjshaandilya3790 6 років тому

    Crystal clear concept visualisation , thanks sir

  • @Somebody_l3164
    @Somebody_l3164 3 роки тому +1

    Nice explanation.. Thank you 🙏🙏

  • @AZaki
    @AZaki 9 років тому +4

    Sometimes a bit hard to understand but the content is perfectly explained.Nice one :) i got a very high grade by using this information in my exam. Thanks Buddy :)

  • @ziaabbas5135
    @ziaabbas5135 5 років тому +1

    Video was very helpful and understanding

  • @rahulsamuel6567
    @rahulsamuel6567 9 років тому

    thanks for video man it helped a lot

  • @renukamaurya1966
    @renukamaurya1966 4 роки тому +1

    Thank you very much sir 🙏 for this video... it's very helpful...... Thanks a lot again👍👍👍👍👍

  • @Aishu-qn5qq
    @Aishu-qn5qq 5 років тому +2

    Very clear explanation...tq

  • @Feeling-blessed
    @Feeling-blessed 8 років тому

    Thanks a lot for supporting this video..

  • @mazinamizba2330
    @mazinamizba2330 5 років тому +2

    great explanation.. thank you

  • @anilamadassery6074
    @anilamadassery6074 6 років тому

    Thank you sir for this vedio... it is very clear. Very well explained.. it is very helpful.

  • @priyankraj3981
    @priyankraj3981 8 років тому

    Great video. Nice orientation to rDNA tech.

  • @aishaansari4819
    @aishaansari4819 8 років тому

    thanks a lot It gonna help me for my boards xamz tomorrow

  • @SalimShaikh-pj9uw
    @SalimShaikh-pj9uw 6 років тому

    Thank you so much for understanding this concept

    • @biologyexams4u
      @biologyexams4u  6 років тому +1

      Welcome Salim, really appreciate your good words, happy to know that it helped

  • @Samuel_Carter_Awakening
    @Samuel_Carter_Awakening Рік тому +1

    I really needed this! Thanks!❤

    • @biologyexams4u
      @biologyexams4u  Рік тому +1

      Hi Chison
      Really glad to hear that the video helped. Just sharing the link of our free ebook on rDNA technology
      www.biologyexams4u.com/2022/02/steps-in-rdna-technology-or-recombinant.html
      Thank you so much

    • @Samuel_Carter_Awakening
      @Samuel_Carter_Awakening Рік тому

      @@biologyexams4u I’ll check it out now! Thank You!

  • @smsmsa9255
    @smsmsa9255 3 роки тому

    Very useful explanation. Thank you sir

  • @ganeshbabu8960
    @ganeshbabu8960 6 років тому

    This is very good video, they gave very good information easily

  • @user-by2gm4cn5h
    @user-by2gm4cn5h Місяць тому +1

    Thanks so much 🙏

  • @radiums7198
    @radiums7198 5 років тому +1

    thanq sir it helped me in de last moment of my exam

  • @arjunmn1240
    @arjunmn1240 8 років тому

    thank u sir it helped a lot

  • @sagarikatalanki9401
    @sagarikatalanki9401 9 років тому

    Very nyc video ....understood very well...tysm

    • @biologyexams4u
      @biologyexams4u  9 років тому +1

      Hi Sagarika Talanki
      Thank you so much for the visit. Really a motivation to do more....

    • @patelnaveen7368
      @patelnaveen7368 9 років тому

      Nic

  • @chetan9886
    @chetan9886 3 роки тому +1

    It was useful sir , thank you

  • @shezadizubeda5274
    @shezadizubeda5274 8 років тому

    thanks u so much fr video

  • @2h6-raviprasad53
    @2h6-raviprasad53 3 роки тому +1

    Excellent explanation

  • @sahlakamaruddeen9326
    @sahlakamaruddeen9326 8 років тому

    very useful video...perfect explanation.......thankyou:-)

  • @YugoLima
    @YugoLima 8 років тому

    Hey, this video helped me with some questions. Thanks!

  • @abrahamvattakunnath8471
    @abrahamvattakunnath8471 7 років тому

    it was very useful. thank you

  • @ashok_creation4549
    @ashok_creation4549 6 років тому

    Very nice explain but you deep explain

  • @hellodear6169
    @hellodear6169 6 років тому

    Your video is soooo clearr.. tq so muchh

  • @aishwaryasen7534
    @aishwaryasen7534 8 років тому

    Nice video but you need to add how the c- chain of insulin is removed

  • @vinayyempiree8738
    @vinayyempiree8738 4 роки тому

    This video is very helpful.
    I'll share it to everyone

  • @basavarajmg9660
    @basavarajmg9660 3 роки тому +1

    Tq so much 👍👍
    Quite interesting...... Sir

  • @supriyakhot4995
    @supriyakhot4995 7 років тому

    thanks it helped a lot

  • @piyushyadav745
    @piyushyadav745 2 роки тому

    Very good explanation 👌🏻 thank you so much

  • @akifaalmas5921
    @akifaalmas5921 3 роки тому

    Thank you Sir video cleared my doubts regarding this particular concept thanks a lot 😊❤❤❤

    • @biologyexams4u
      @biologyexams4u  2 роки тому

      Hi Akifa
      Glad to hear that
      Please consider subscribing: bit.ly/3kG2kKf

  • @hamayljaffri3982
    @hamayljaffri3982 7 років тому

    Very helpful and useful 👌

  • @mitochondria720
    @mitochondria720 4 роки тому

    That was helpful Sir_I'm cleared now_Thank you.

    • @biologyexams4u
      @biologyexams4u  4 роки тому

      Glad it helped....Thank you for the support.... Muftima

  • @britneyvolckaert9281
    @britneyvolckaert9281 4 роки тому

    Excellent explanation! Thank you :)

    • @biologyexams4u
      @biologyexams4u  4 роки тому

      Glad it was helpful! Thank you so much...Stay healthy and Stay safe:)

  • @dr.rituchandra7088
    @dr.rituchandra7088 4 роки тому

    Thanks a lot for the video. I'd really appreciate if you could add subtitles to the videos, though most of what you speak is on the screen only, I think it would be helpful.

  • @GrammeStudio
    @GrammeStudio 10 років тому

    What are the steps in Recombinant DNA tech?
    1. Cut bacterial plasmid (vector) using restriction enzyme
    2. Ligase gene of interest onto the gap of bacterial plasmid to get Recombinant DNA
    3. Insert altered vector into bacterial host
    4. Allow for multiplication into colony
    5. Select for Transformed bacterial cell with recombinant (altered) vector through Selective Medium, etc

  • @Alberuniii
    @Alberuniii 2 роки тому

    Kia kehna yr zbr 10st
    Thanks

    • @biologyexams4u
      @biologyexams4u  2 роки тому

      Hi Abdul Aziz
      Thank You so much
      Please consider subscribing: bit.ly/3kG2kKf

  • @tarannumkhatoon106
    @tarannumkhatoon106 7 років тому +1

    ur vidio is v. nice i really realyyyyyy like ur vidio 👌👌👌👌

  • @muhammadbilal-zk8wm
    @muhammadbilal-zk8wm 8 років тому

    great job

  • @taslimwazir9694
    @taslimwazir9694 6 років тому

    Bundle of thanks sir g...

    • @akuladevender8959
      @akuladevender8959 5 років тому

      plasmid is used because easy to remove it from bacteria and insert it again

  • @saksham631
    @saksham631 9 років тому +11

    helped alot

    • @biologyexams4u
      @biologyexams4u  9 років тому +4

      Really appreciate your nice words. Really a motivation to do more.....Thank you so much for the visit and comment

  • @chandigarhdiyaariya2470
    @chandigarhdiyaariya2470 7 років тому +1

    u are superb nd u'r video amazinggggggggggg ☺☺☺☺☺☺☺☺☺☺☺☺☺✔

  • @tradalhmoud5345
    @tradalhmoud5345 10 років тому

    Thnx it really helped

  • @Bindasjhakyass
    @Bindasjhakyass 9 років тому

    thanks

  • @ghousiyafatima2319
    @ghousiyafatima2319 3 роки тому

    Thank u sir!
    This was really helpful...

    • @biologyexams4u
      @biologyexams4u  2 роки тому

      Hi Ghousiya
      Glad to hear that
      Please consider subscribing: bit.ly/3kG2kKf

  • @selinaiqbal229
    @selinaiqbal229 6 років тому

    so useful!! thank you

  • @md.monirulislam1765
    @md.monirulislam1765 6 років тому

    useful video, thanks.

  • @annathomas8569
    @annathomas8569 9 років тому

    thank you :) helped a tonne!

  • @rinkychowrasia007
    @rinkychowrasia007 8 років тому

    LUCID EXPLANATION!!!!! THANKS

  • @anjanatacholikadathanadan47
    @anjanatacholikadathanadan47 6 років тому

    This was really helpfullll😀😀😀😀

  • @xiantitan6841
    @xiantitan6841 8 років тому

    very nice tutorial!

  • @akmalabdullah3451
    @akmalabdullah3451 8 років тому

    Help a lot. But I suggest for your next video, be more specific. Such as how blotting work and else.