Steps Involved in the production of Recombinant Insulin. How Recombinant Insulin is produced?
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- Опубліковано 20 лип 2024
- This video explains How recombinant insulin is produced?
00:00 Introduction
00:45| Why Humulin or recombinant insulin is a milestone in
rDNA technology?
What were the previous insulin sources?
Advantages of recombinant insulin
02:15 | Discovery of Structure of Insulin by Frederick Sanger
02:50| 6 Steps in rDNA technology
03:43 | Step 1: Identification & Isolation of Insulin gene
04:25 |Step 2: Insertion of this isolated gene in a suitable expression vector
vector used for recombinant insulin production
06:30| Step 3: Introduction of this vector into a suitable host; E.coli
Hosts used for recombinant insulin production
07:42| Step 4: Selection of the transformed host cell with insulin gene
08:45| Step 5: Expression of insulin gene in the host
09:42| Step 6: Purification of insulin protein
What is recombinant fusion protein? and its uses
11:30| Summary of Steps Involved in the production of Recombinant Insulin
▶Steps in recombinant DNA technology • Steps in Recombinant D...
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Thank you very much.☺️
nice video. Thanks. But what is the importance of galactosidase here why it should be placed next to insulin.
Wonderful presentation. Thank you!!
You're very welcome!
Just sharing our playlist of Biotechnology videos for your reference
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Excellent presentation..
helped me for my aignment
thank you
Thank u sir.. Really very helpful
Thank you! This video has helped me out a lot for microbiology!!
Thanks for the this
I'm so going to eat up my recombinant DNA technology presentation
excellent presentation
The DNA soup of humans is far more complex than 99% of human can understanding. But it's taught, as 'basic rectilinear rules and guidelines'
Thankyou
thank you this video help to me for campus lesson understand
Glad it helped!
Thank you sir
So nice of you
Sir I have a question, is recombinant insulin and biosimilar insulin are same thing or different? Please answer me 🙏
Recombinant insulin refers to insulin that was produced by inserting the genes for the required molecular machinery into plasmids and then expressing them within prokaryotic organisms at scale.
Biosimilar insulin is produced with recombination but the insulin is not identical to human insulin. This would be insulin like complexes that very closely match human insulin except for a few differences. Differences likely introduced for efficient production.
Recombinant = plasmid expression of target protein(s)
Biosimilar = molecule/protein/etc that serves same function as the target in human but differs slightly in structure.
Sir why humulin doesn’t cause allergic reaction ?
In humulin, we used human insulin gene to make insulin which is same as insulin produced in vivo or within our body. So no allergic reaction. Hope i clarified your doubt. Thank you so much
Does rDNA in insulin change your DNA?
Great teacher
Thank you so much for your nice words
Best Wishes:)
Why are u asking money for certificate sir?
Hi Samyajit
Would you please clarify...we are not asking money from any one...our only revenue is from ads on this channel. Thank you
@@biologyexams4u I have completed the module 1 and 2 of recombinant DNA technology but afterwards it demand money for soft copy
@@samyajitpatra3408
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