There was more content in the second paper that i was going to cover, but the video was getting quite long! So briefly here...In the second paper they also assess SA-beta-gal staining in the brain (so using, albeit one, maker for senescence) and saw a reduction both with NBE and a senolytic compound (ABT-263). However, ABT 263 had limited effects on neuroinflammation and did not enhance hippocampal neurogenesis in the old mice compared to NBE. So, *suggests* that NBE acts partially through senolytic activity, but rejuvenation not just from reducing senescence....interesting!
Excellent, excellent coverage of the Conboy NBE results. They are potentially extremely exciting. I also like the questions you posed at the end. There is still much to be determined about what is happening and the extent of the changes and you make a great point about needing to study females as well as males. I also like your comment about the 2nd paper having information about senescence. And, I will check out your earlier video about how the proteome changes with age. That sounds really interesting. Thanks for doing this video Eleanor! You are doing a fantastic job!
Very informative, thank you! I'm glad that longevity research is finally getting some steam. This shit is the future and you're a godsend for getting this information out there
thanks for covering this, i think the Conboy's work is some of the most interesting in the field. apparently they are finishing up Phase 2 trials in humans right now and are planning to launch a company with Phase 3 trials around May. Irina Conboy mentioned it in some of her talks on UA-cam, it's very exciting
Thanks for redrawing the feedback diagram. I glossed over that in the original paper because it seemed overly complex. Since TPE is being used by people it would be interesting to do the same protein level measurements over time periodically to see the longer term effects.
Wow! and bravo! You are a world-class communicator! I have rarely heard/witnessed anyone who achieves so clean a signal to noise ratio, and without any of the gaps usually found when someone who knows the topic "too well" forgets that the listener needs all the "bits" that connect and complete the content, and leaves some of that vital linking material out because it is so well known to the speaker that it becomes to him/her almost subconscious and "internalized", and then mistakenly left out of "the lesson". Again, excellent work. I subscribed instantly.
Thanks. Another excellent presentation. Did not pick up that platelets were also carried off with plasma when I read the first paper. BTW, my local blood bank solicits plasma donation and platelets, as well as whole blood. They take 20% of plasma in one go and replace with saline. That should reduce albumin by about 10% which should not be a problem with a healthy liver. Can repeat after one month. I've been a whole blood donor for a while but am going to switch over to plasma for 2 or 3 donations and monitor using standard blood markers which includes liver and kidney function. Seems that any repair of damage would be long lasting while normal aging of tissues might resume fairly soon.
@@lastraven7205 Too early to say. First CBC/CMP blood panel yesterday and 4th donation tomorrow,. Will be tracking blood work more rigorously in future. Tracking many parameters, especially albumin and liver function. After getting into the process and getting my real numbers (mls of plasma donated) I've learned that my donation center does not always solicit plasma but does routinely want platelets. Platelets can be donated every 2 weeks but along with it goes plasma. You can do a single or double platelet in which case you will loose (for my ht ad wt of 5'8 and 170 lbs) ~180 mls or ~350 mls. Plasma plus platelets is also done at every other visit (i.e. once per 4 weeks) which results in about 470 mls of plasma loss (~16% loss). Have not given plasma only which would be 600 mls for me due to their need for platelets. Last donation started noting actual volume which tech takes from machine so will have better numbers in the future. Appears I will off load about 400 mls plasma every 2 weeks, on average over time (~13% plasma loss). Maybe this will be enough that over time gene expression is positively modulated as shown by Conboys for single large volume TPE. I will pay close attention to making sure my albumin level does not go down and stay down as they do not replace albumin as is done in TPE, and higher albumin is associated with better health and longevity. I hope to improve liver and kidney function over time, as indicated by basic CBC/CMP blood work, as their function has widespread health impacts as can be measured on the basic blood panels. I don't expect to see any outward changes, at least not soon. It would be a positive indicator if some of my grey hair reverted or my tinnitus went away. As I am 67 yo I am mainly hoping that fibrotic tissues are partially reversed and senescent cell burden is lowered, also as indicated by Conboys. But I am one person doing many things so not scientifically rigorous at all. Worse case I help people in need of blood products, and that's a good thing.
@@peterz53 How goes your blood testing, going to start donating plasma instead of whole blood this week. Whole blood donations drops my already low white blood cell counts. I'm 64 yo and very healthy and looking for some additional age reversal options. TIA.
@@DTMFJeff I am going into CSL plasma donation center for first time on Friday (in 2 days) Have had problems finding a plasma only center due to my age of 67, but CSL says it's not an issue. In any case, I have hopes that all will go well, and I can donate. Should be in range of 600 to 800 mls, or up to 27% of plasma only in one go. They allow 2 donations a week. If I am accepted, I will do another the following Monday then take a 4 week break to deal with other issues. My last two blood bank donations were single platelet donations which took about 470 mls of plasma on Dec 26th and Jan 23rd. Will be getting blood drawn for testing Friday morning before donation, then again in a month. Last blood work in December showed best ever liver function (AST and ALT) and improved RDW, but need to track blood chem better. By that time I had donated about 4 liters of plasma in 2021 as part of platelet donation. The Conboy lab work indicated that NBE lasts at least a month, so I plan to do one plasma donation a month and one platelet per month for remainder of 2022 by which time any positive tissue effects (grey hair, skin, vision, etc.) should become apparent.
With all the major recent advances in the understanding of the process of aging, I would not be surprised if a first treatment for anti aging was revealed and released within the next ONE to TWO YEARS.
I remember when I was your age in 2001 and I knew this stuff was coming from a book I'd read on the subject in the late 90s, but nobody was talking about it. I even wrote a paper on anti-aging research for a university biology class. The prof did talk a little about anti-aging compounds though. He was in his 60s and talked about a supplement he was taking and did a headstand in class to show he still had it :) However, I also knew true results were some time in the future. Now I am exactly 20 years older than you and watching the research unfold with great interest, but rather jealous that you get to be the same age as I was when I knew this was coming but the field was nowhere even close to where it is today! Damn youuuuu.... :)
@@SlamminGraham If the 'bad' proteins build up over a lifetime, and a portion of them are removed from the body, when the body regenerates new plasma, the new plasma doesn't have the 'bad' proteins. This is the 'dilution' that is beneficial. The bad proteins are inhibitory to the youthful proteins. In the absence of bad proteins, (or less of them), the genes are free to express more youthful protein profiles. At least that is how I understand it from the Conboy's paper. They had a heat map that illustrated the youthful proteome, and the aged proteome, as well as the therapeutic plasma dilution (TPE) proteome. The TPE heat map looked like a youthful one. This has many downstream effects that are beneficial.
Peer reviewed study published in "Alzheimer's & Dementia": A randomized, controlled clinical trial of plasma exchange with albumin replacement for Alzheimer's disease: Primary results of the AMBAR Study
When plasma donation is done in the US, removed plasma volume is typically replaced with saline only, as one of the products sought by these for profit corporations is albumin. Obviously there is more to be understood about the theraputic mechanisms/benifits/negatives of TPE. I wonder though, could donating plasma and amending and supplementing the diet to enhance the production of albumin in the liver empart any theraputic benefit? In any of these studies did they compare just saline solution vs. saline +5% albumin? I guess its up to me to find out. Thank you for the channel, i always learn something.
@@revilodraw88 Nothing noticeable that I can actually feel. But in every donation, a lot of gross fat is taken out so that helps keep my cholesterol down. All donors have to take in an adequate amount of protein too. We are replenished with sodium citrate (anticlotting solution). That's what I wanted to know if that has any anti-aging effect on us.
@@t1relaxation Interesting. I donate roughly every fortnight (haven't been doing it for all that long though, 8 donations in total thus far). I don't believe I receive sodium citrate, just saline. Which country are you in? I'm in Australia.
It may be that exosomes and other micro vesicles exists in albumin and it is not neutral like everybody is assuming.... nevertheless you are absolutely correct that we have to remove all this "trash" out of our blood system (and our body as a whole) as it is probably major contributor to aging...
Ironic that they are removing the prp when so many treatments seem to apply a concentrated dose to targeted areas. I wonder if the two can be combined synergistically or if old prp is just not good for healing anymore...
I would think we would accumulate "forever chemicals" and other inert sludge in plasma - because cells are routinely recycled, at which point any inert sludge would be released into plasma? At SOME point the ability of plasma to carry its load of inert sludge would be exceeded - kinda like what eventually happens in an engine when you never change the oil. You speculate that the inert sludge is proteins - but there are LOTS of other things that find their way into blood - like aluminum hydroxide activator used in some vaccinations, etc. I would think a major load of inert sludge might show itself as impaired kidney function, like maybe a GFR
I'm definitely adding to the voices asking for you to look at Dr Harold Katchers work. His M5 elixir has dropped the age of middle aged rats 🐀 by 54%. Just about to kick off independent trials on dogs and monkeys 🐒. Independent trials are what we all want to see. Harald and his small team apparently cracked this particular nut. Anyway the spotlight needs to be focused on Dr Katcher so no time is wasted getting M5 into humans to stop the "lucky" humans dieing a slow miserable and degrading painful death!
Is this process kind of the opposite of what happens when you give blood? When you give blood they keep the part that this experiment is giving back and they give back the part that this experiment is removing.
improve the sound, you are trying to convey scientific facts and concepts, to an audience, please slowdown and speak legibly. don't be running while talking. Thank you.
There was more content in the second paper that i was going to cover, but the video was getting quite long! So briefly here...In the second paper they also assess SA-beta-gal staining in the brain (so using, albeit one, maker for senescence) and saw a reduction both with NBE and a senolytic compound (ABT-263). However, ABT 263 had limited effects on neuroinflammation and did not enhance hippocampal neurogenesis in the old mice compared to NBE. So, *suggests* that NBE acts partially through senolytic activity, but rejuvenation not just from reducing senescence....interesting!
You need to cover Harold Katcher's recent work on true epigenetic rejuvenation in mice. Their preprint paper with Steve Horwath is available online.
Katchers work looks very exciting
Your videos just get better and better! Thanks - much appreciated...
Excellent, excellent coverage of the Conboy NBE results. They are potentially extremely exciting. I also like the questions you posed at the end. There is still much to be determined about what is happening and the extent of the changes and you make a great point about needing to study females as well as males. I also like your comment about the 2nd paper having information about senescence. And, I will check out your earlier video about how the proteome changes with age. That sounds really interesting. Thanks for doing this video Eleanor! You are doing a fantastic job!
Thanks Neil!!
Very informative, thank you! I'm glad that longevity research is finally getting some steam. This shit is the future and you're a godsend for getting this information out there
thanks for covering this, i think the Conboy's work is some of the most interesting in the field. apparently they are finishing up Phase 2 trials in humans right now and are planning to launch a company with Phase 3 trials around May. Irina Conboy mentioned it in some of her talks on UA-cam, it's very exciting
Thanks for redrawing the feedback diagram. I glossed over that in the original paper because it seemed overly complex. Since TPE is being used by people it would be interesting to do the same protein level measurements over time periodically to see the longer term effects.
Can you do a episode on GH, sermorelin et. al. for anti-aging? Loving your content the videos are getting better.
Wow! and bravo! You are a world-class communicator! I have rarely heard/witnessed anyone who achieves so clean a signal to noise ratio, and without any of the gaps usually found when someone who knows the topic "too well" forgets that the listener needs all the "bits" that connect and complete the content, and leaves some of that vital linking material out because it is so well known to the speaker that it becomes to him/her almost subconscious and "internalized", and then mistakenly left out of "the lesson".
Again, excellent work. I subscribed instantly.
Thanks. Another excellent presentation. Did not pick up that platelets were also carried off with plasma when I read the first paper. BTW, my local blood bank solicits plasma donation and platelets, as well as whole blood. They take 20% of plasma in one go and replace with saline. That should reduce albumin by about 10% which should not be a problem with a healthy liver. Can repeat after one month. I've been a whole blood donor for a while but am going to switch over to plasma for 2 or 3 donations and monitor using standard blood markers which includes liver and kidney function. Seems that any repair of damage would be long lasting while normal aging of tissues might resume fairly soon.
How is it working out for you?
@@lastraven7205 Too early to say. First CBC/CMP blood panel yesterday and 4th donation tomorrow,. Will be tracking blood work more rigorously in future. Tracking many parameters, especially albumin and liver function. After getting into the process and getting my real numbers (mls of plasma donated) I've learned that my donation center does not always solicit plasma but does routinely want platelets. Platelets can be donated every 2 weeks but along with it goes plasma. You can do a single or double platelet in which case you will loose (for my ht ad wt of 5'8 and 170 lbs) ~180 mls or ~350 mls. Plasma plus platelets is also done at every other visit (i.e. once per 4 weeks) which results in about 470 mls of plasma loss (~16% loss). Have not given plasma only which would be 600 mls for me due to their need for platelets. Last donation started noting actual volume which tech takes from machine so will have better numbers in the future. Appears I will off load about 400 mls plasma every 2 weeks, on average over time (~13% plasma loss). Maybe this will be enough that over time gene expression is positively modulated as shown by Conboys for single large volume TPE. I will pay close attention to making sure my albumin level does not go down and stay down as they do not replace albumin as is done in TPE, and higher albumin is associated with better health and longevity. I hope to improve liver and kidney function over time, as indicated by basic CBC/CMP blood work, as their function has widespread health impacts as can be measured on the basic blood panels. I don't expect to see any outward changes, at least not soon. It would be a positive indicator if some of my grey hair reverted or my tinnitus went away. As I am 67 yo I am mainly hoping that fibrotic tissues are partially reversed and senescent cell burden is lowered, also as indicated by Conboys. But I am one person doing many things so not scientifically rigorous at all. Worse case I help people in need of blood products, and that's a good thing.
@@peterz53 How goes your blood testing, going to start donating plasma instead of whole blood this week. Whole blood donations drops my already low white blood cell counts. I'm 64 yo and very healthy and looking for some additional age reversal options. TIA.
@@DTMFJeff I am going into CSL plasma donation center for first time on Friday (in 2 days) Have had problems finding a plasma only center due to my age of 67, but CSL says it's not an issue. In any case, I have hopes that all will go well, and I can donate. Should be in range of 600 to 800 mls, or up to 27% of plasma only in one go. They allow 2 donations a week. If I am accepted, I will do another the following Monday then take a 4 week break to deal with other issues. My last two blood bank donations were single platelet donations which took about 470 mls of plasma on Dec 26th and Jan 23rd. Will be getting blood drawn for testing Friday morning before donation, then again in a month. Last blood work in December showed best ever liver function (AST and ALT) and improved RDW, but need to track blood chem better. By that time I had donated about 4 liters of plasma in 2021 as part of platelet donation. The Conboy lab work indicated that NBE lasts at least a month, so I plan to do one plasma donation a month and one platelet per month for remainder of 2022 by which time any positive tissue effects (grey hair, skin, vision, etc.) should become apparent.
With all the major recent advances in the understanding of the process of aging, I would not be surprised if a first treatment for anti aging was revealed and released within the next ONE to TWO YEARS.
I remember when I was your age in 2001 and I knew this stuff was coming from a book I'd read on the subject in the late 90s, but nobody was talking about it. I even wrote a paper on anti-aging research for a university biology class. The prof did talk a little about anti-aging compounds though. He was in his 60s and talked about a supplement he was taking and did a headstand in class to show he still had it :)
However, I also knew true results were some time in the future. Now I am exactly 20 years older than you and watching the research unfold with great interest, but rather jealous that you get to be the same age as I was when I knew this was coming but the field was nowhere even close to where it is today! Damn youuuuu.... :)
Seriously thinking about donating plasma. It could be a low risk way of getting a subtle regenerative benefit.
I don't think so. This won't affect the concentrations of things in your blood at all, which is the point of either dilution or parabiosis.
@@SlamminGraham If the 'bad' proteins build up over a lifetime, and a portion of them are removed from the body, when the body regenerates new plasma, the new plasma doesn't have the 'bad' proteins. This is the 'dilution' that is beneficial. The bad proteins are inhibitory to the youthful proteins. In the absence of bad proteins, (or less of them), the genes are free to express more youthful protein profiles. At least that is how I understand it from the Conboy's paper. They had a heat map that illustrated the youthful proteome, and the aged proteome, as well as the therapeutic plasma dilution (TPE) proteome. The TPE heat map looked like a youthful one. This has many downstream effects that are beneficial.
Wouldn't this mean its bad to get blood transfusions from older people?
@@lastraven7205 From a longevity perspective, yes.
Peer reviewed study published in "Alzheimer's & Dementia":
A randomized, controlled clinical trial of plasma exchange with albumin replacement for Alzheimer's disease: Primary results of the AMBAR Study
Blood letting for old people is now cutting edge science. Cool
Great video, well explained 👍 .. any updates on this? Has it hit the commercial markets yet?
thanks for your knowledge, we need peeople like you :)
Awesome level of detail explained really well. Thank you. 👍😊
I learned something just not sure what it was. Excellent video.
I'm curious if removing these bad factors (or diluting them) could cause upregulation of them after they are removed.
When plasma donation is done in the US, removed plasma volume is typically replaced with saline only, as one of the products sought by these for profit corporations is albumin.
Obviously there is more to be understood about the theraputic mechanisms/benifits/negatives of TPE. I wonder though, could donating plasma and amending and supplementing the diet to enhance the production of albumin in the liver empart any theraputic benefit? In any of these studies did they compare just saline solution vs. saline +5% albumin? I guess its up to me to find out. Thank you for the channel, i always learn something.
"it has whopping 130 views, 10 of them are mine"
Ms. Sheekey, does this mean that donating plasma is good for anti-aging. I donate about 7L of plasma/month.
7L per month?!
@@revilodraw88 Yes, I donate 8X/month.
@@t1relaxation Wow, that's a lot!. Have you noticed any visible rejuvenation?
@@revilodraw88 Nothing noticeable that I can actually feel. But in every donation, a lot of gross fat is taken out so that helps keep my cholesterol down. All donors have to take in an adequate amount of protein too. We are replenished with sodium citrate (anticlotting solution). That's what I wanted to know if that has any anti-aging effect on us.
@@t1relaxation Interesting. I donate roughly every fortnight (haven't been doing it for all that long though, 8 donations in total thus far). I don't believe I receive sodium citrate, just saline. Which country are you in? I'm in Australia.
It may be that exosomes and other micro vesicles exists in albumin and it is not neutral like everybody is assuming.... nevertheless you are absolutely correct that we have to remove all this "trash" out of our blood system (and our body as a whole) as it is probably major contributor to aging...
Ironic that they are removing the prp when so many treatments seem to apply a concentrated dose to targeted areas. I wonder if the two can be combined synergistically or if old prp is just not good for healing anymore...
I would think we would accumulate "forever chemicals" and other inert sludge in plasma - because cells are routinely recycled, at which point any inert sludge would be released into plasma? At SOME point the ability of plasma to carry its load of inert sludge would be exceeded - kinda like what eventually happens in an engine when you never change the oil. You speculate that the inert sludge is proteins - but there are LOTS of other things that find their way into blood - like aluminum hydroxide activator used in some vaccinations, etc.
I would think a major load of inert sludge might show itself as impaired kidney function, like maybe a GFR
I'm definitely adding to the voices asking for you to look at Dr Harold Katchers work. His M5 elixir has dropped the age of middle aged rats 🐀 by 54%. Just about to kick off independent trials on dogs and monkeys 🐒. Independent trials are what we all want to see.
Harald and his small team apparently cracked this particular nut. Anyway the spotlight needs to be focused on Dr Katcher so no time is wasted getting M5 into humans to stop the "lucky" humans dieing a slow miserable and degrading painful death!
How is it 2022 and researchers are still conducting studies using only male mice?
I have heard that similar effects, or benefits, can be had by donating blood, which forces new blood to be generated. Thoughts?
I donate double red blood cell with a return to my white blood cell and plasma every 3 months . That’s my theory for donating .
Really good video!!
Great vid! How would NBE compare to dialysis?
What about phages and viruses and other abundant organisms in plasma . Its akin to colonic wash
Blood letting is good for you?
Ugh I hate needles and blood draws / phlebotomy.
Could you please tell me how much blood needed to be exchanged to see such effect? Many Thanks!
Is this process kind of the opposite of what happens when you give blood? When you give blood they keep the part that this experiment is giving back and they give back the part that this experiment is removing.
Not quite, as typically when you donate blood, you donate blood (so blood cells & plasma)!
2:10 🎯
The life is in the blood
I donate Platelets twice a month, curious the affect of just donating platelets
Why not plasma?
@@bugrasoner Platelets only last five days and cancer patients need them, the donation center says platelets please, please
improve the sound, you are trying to convey scientific facts and concepts, to an audience, please slowdown and speak legibly. don't be running while talking. Thank you.
Sounds gross.