Enzymes (Part 3 of 5) - Lineweaver Burk Plot - Double Reciprocal Plot

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  • Опубліковано 16 гру 2024

КОМЕНТАРІ • 124

  • @sogazelle
    @sogazelle 10 років тому +103

    Mahfoud, God Bless you...I just failed my biochem first exam because I didn't grasp the concepts from the way the teacher explains them. And we started the enzyme chapter last week and I had a headache throughout the whole 2 hour class even after reading the book. But I just spent the most beautiful hour listening to your 5 videos on enzymes and I cried from the joy of how amazingly and simply you explained the concepts and I just understood the whole chapter. Thank you so much! You are blessed with the mind of a real teacher. I hope that because of you, I will pass my biochem class. Thank you.

    • @MoofUniversity
      @MoofUniversity  10 років тому +23

      sogazelle Wow! I'm going through a bunch of comments saying "you're welcome," and I come across these incredibly kind words from you! THANK YOU for the kind words! I'm very happy to have helped you understand biochemistry! :]

  • @001button
    @001button 9 років тому +37

    saving my life one lecture at a time :)

  • @DowaKaday
    @DowaKaday 10 років тому +4

    all your videos give me an understanding of biochemistry in just a week when i cant understand 3 months of lectures from my lecturer. Bless you my good man! :)

    • @MoofUniversity
      @MoofUniversity  10 років тому +2

      DowaKaday Lol. Wow! I'm happy to have helped, dude!

    • @MoofUniversity
      @MoofUniversity  10 років тому +2

      DowaKaday ....and thanks for the blessings! :]

  • @randomdude4628
    @randomdude4628 7 років тому +1

    Thanks to you, cramming is no longer what it used to be. This is like a TV show, only more engaging.

    • @MoofUniversity
      @MoofUniversity  7 років тому

      More engaging than a TV show? Really? What sort of TV shows are you watching, dude? Haha! There are some pretty great shows out there.

    • @randomdude4628
      @randomdude4628 7 років тому

      Haha! That was meant with the utmost sincerity. With TV shows I find myself playing around on my phone within the first 10 minutes, but your video lessons are very interesting and genuinely make me want to watch more. Thank you for the great videos! :)

    • @MoofUniversity
      @MoofUniversity  7 років тому

      Lol. Well, I'm glad you find my content that engaging and interesting. Thanks so much for watching and for really enjoying them.
      In case you're interested in taking TV show suggestions, here are some that I'm into at the moment and/or have enjoyed in the more recent past: Master of None, Narcos, Game of Thrones, Better Call Saul, Sherlock, and Peaky Blinders. If none of those float your boat, please feel free to continue watching my videos. Haha!

  • @amberlange9082
    @amberlange9082 8 років тому +11

    You're seriously getting me though biochemistry, thank you so much!

  • @subsixtynk95
    @subsixtynk95 7 років тому +6

    This man is a LEGEND! Ohhhh my GOD, the clarity is unreal!! Thank you so much!

  • @marcelkhoury6768
    @marcelkhoury6768 10 років тому +6

    This is the best thing i have ever found on the internet and i am eternally grateful Mahfouf. Thank you.

    • @MoofUniversity
      @MoofUniversity  10 років тому +1

      Mark Khoury Hahaha! What a compliment! Thanks, dude!

  • @matthew_bang
    @matthew_bang 5 років тому +1

    i have a midterm coming up in less than a week and you are helping me so much

  • @ngrubs55
    @ngrubs55 8 років тому

    Holy crap, it's all so clear to me now. I swear you have saved my blood pressure. I feel like I owe you for how relieved you have made me.

  • @bhupendramahawar9010
    @bhupendramahawar9010 8 років тому

    I think some people are trying to get the clinical explanation for this and not the mathematical one. I came here to watch the clinical part too.... but this is probably one of the best derivation i have seen and i wish i had watched this vid when i was learning this stuff for the first time! Great explanation. Thank you so much !

  • @MarzNet256
    @MarzNet256 8 років тому

    I learned all this in cell biology about 30 years ago. You do a very good job explaining it.

  • @ayesha2014
    @ayesha2014 3 роки тому +1

    I have an exam in 2.5 hours. Watching this because i just couldn't understand the x-intercept thingy. THANK YOU !!!!

    • @MoofUniversity
      @MoofUniversity  3 роки тому +1

      You're very welcome! I hope you did well on the exam!

    • @ayesha2014
      @ayesha2014 3 роки тому

      @@MoofUniversity I did !!! Thanks once again :)

    • @MoofUniversity
      @MoofUniversity  3 роки тому

      @@ayesha2014 Awesome! You're very welcome!

  • @Evrce
    @Evrce 10 років тому +1

    Dang! i just graduated BCH from UCR and some friends asks me for help. Wish i found you sooner because you're very clear and helpful.

    • @MoofUniversity
      @MoofUniversity  10 років тому

      ***** Dang! If they need help, you can definitely have them contact me via email. :]

  • @Silvergladegirl
    @Silvergladegirl 10 років тому +1

    Thank you SO MUCH for this video and the others on biochemistry related stuff. YOU sir, are the reason I will pass biochemistry. You're my hero, seriously.

    • @MoofUniversity
      @MoofUniversity  10 років тому

      Wow! Thanks for the kind words, and you're very welcome! :]

  • @hb6839
    @hb6839 6 років тому +1

    I thank heavens for the day I found your videos as they'll help me become a good doctor soon, god bless helpful people like you.

    • @MoofUniversity
      @MoofUniversity  6 років тому +1

      RIght on. Thank you, and good luck, Doc!

  • @jonqn07
    @jonqn07 8 років тому +1

    thank you Moof University. You helped me understand this! I have no clue looking at the book here. Thanks for the little Vmax etc..explanation too.

  • @urockit2011
    @urockit2011 8 років тому +1

    A much better explanation than Khan Academy's! Thank you!

  • @ayesha2014
    @ayesha2014 3 роки тому +1

    Thank you for making the math in biochem easier :)

  • @erkanbatti
    @erkanbatti 8 років тому

    you have no idea how much you are helping me. thank you so much.

  • @sharshal465
    @sharshal465 6 років тому

    Awesome video, Appreciate the way you take your time to properly explain what is going on here.

  • @yomayranegron-torres2428
    @yomayranegron-torres2428 7 років тому

    Amazing explanations!!.. You are helping me to excel this part of my USMLE Step 1 !!!

  • @alphamsesay333
    @alphamsesay333 3 роки тому

    This video is all I needed for my presentation. Thanks and God bless you

  • @chelseajenkins7325
    @chelseajenkins7325 7 років тому +1

    You seriously saved my life!!!!!!

  • @camerongj1
    @camerongj1 9 років тому

    This was a fantastic explanation of the L-B plot! Thank you very much.

  • @FortisAquila14
    @FortisAquila14 9 років тому +2

    God bless u and this day when i found you!

  • @Rawmon94
    @Rawmon94 9 років тому +1

    AWESOME! thanks! gonna ace that biochem test!

  • @MsMopanda
    @MsMopanda 9 років тому +1

    Your wonderful. You should be a professor . Your calm, and adorable. I love you side comments. Thank you for saving my grade

    • @MoofUniversity
      @MoofUniversity  9 років тому

      MsMopanda Lol. Thank you so much! You're far too kind!

  • @edgarmalesa2367
    @edgarmalesa2367 9 років тому +1

    thanks man.. you the best ... like the way you explain things.. make them seem easy ... thanks again

  • @jo-jr2dv
    @jo-jr2dv Рік тому

    youre very good at explaining things

  • @dalaeloubeidi6254
    @dalaeloubeidi6254 9 років тому

    Great job! I think you did a really good job of explaining all these concepts!

  • @m.t1446
    @m.t1446 5 років тому

    The last 5 hours of my life in 7 minutes. Not all heroes wear capes

  • @NoorKhan-tb9dl
    @NoorKhan-tb9dl 5 років тому +1

    Dude you are the FREAKING BEST!!

  • @LetUsBeLazy
    @LetUsBeLazy 7 років тому +1

    This video helped so much! Thank you!

  • @naamaxo
    @naamaxo 8 років тому +2

    Hi thank you so much fre this! i just have one question. How is it possible to get a negative 1/[S] value in the first place? Why is the x-intercept in the 2nd quadrant to begin with? Thanks!

  • @amalabdallah2885
    @amalabdallah2885 9 років тому +1

    I'm so grateful for this video.
    thank you soo much!!!!

  • @1Shoda
    @1Shoda 10 років тому

    Why didn't I look up up for u earlier??? Now I am stalking all ur kinetics video as I study for final!!!!

    • @MoofUniversity
      @MoofUniversity  10 років тому

      sneha hoda I don't know, dude. You should tell your study buddies about me and my channel, though! Lol. I hope you did well on that final.

  • @Gera1988mtl
    @Gera1988mtl 7 років тому

    Excellent presentation and explication, thanks man

  • @StanleyWilhelm
    @StanleyWilhelm 8 років тому

    thanks for making this series

  • @treesageorge3258
    @treesageorge3258 8 років тому +1

    well explained sir!! thanks :) but how did u derive "x intercept"?? (dint understnd that part)

  • @sabrinarashidi3226
    @sabrinarashidi3226 Рік тому

    This was so helpful but i have question for lineweaver burk plot does it have to be that straight line because my enzyme esterase graph it has curve ?

    • @MuthuThala-ih2wu
      @MuthuThala-ih2wu 7 місяців тому

      Lineweaver developed this plot along with mathematicians to reduce the complexity for biology
      Students.
      In order to calculate for graphs with curves it needs calculus
      But for straight line graphs it's easy to calculate graph parameters..
      So all lineweaver plots will have straight line function
      U might have seen michealis menten graph for esterase...

  • @thailizvazquez6830
    @thailizvazquez6830 6 років тому

    Thank you so much!!! Very helpful video! I could easily understand the graphs! :)

  • @YoungBuddhaEzuk
    @YoungBuddhaEzuk 5 років тому

    DUDE yes, thx so much 🤘🏽🤘🏽🙏🏽🙏🏽🔥🔥(Biochemistry)

  • @maria11135
    @maria11135 9 років тому +1

    you are legendary!!!

  • @violinsheetmusicblog
    @violinsheetmusicblog 8 років тому +2

    How is -1/Km derived?

  • @allaalbonijim2748
    @allaalbonijim2748 8 років тому +1

    THIS IS AMAZING!!!!

  • @sphamguyo6551
    @sphamguyo6551 8 років тому +1

    thank you

  • @emiliabri1781
    @emiliabri1781 5 років тому +1

    Thank you!

  • @rareflower88
    @rareflower88 9 років тому +1

    Thank you - really helpful!

  • @ambrosemadongonda7630
    @ambrosemadongonda7630 2 роки тому

    Thank you you have made it easier

  • @mustachegurl1714
    @mustachegurl1714 7 років тому

    if the km is always positive. How can I plot it?

  • @1991AngelAyala
    @1991AngelAyala 8 років тому

    this video is magic

  • @shazwannaim8543
    @shazwannaim8543 8 років тому

    Really helped me to understand...thanks!!!!

  • @travisscott5779
    @travisscott5779 7 років тому

    Thanks from Saudi Arabia i love u man u made easy for me may allah bless u

  • @karinaaguilar4941
    @karinaaguilar4941 2 роки тому

    Was here in 2018 when I took undergraduate biochemistry, from your videos I made an A in the class! Now here in 2022 for review because I have started a Biochemistry PhD and we are taking the class as our 1st year curriculum. Thank you!!!!!

    • @MoofUniversity
      @MoofUniversity  2 роки тому

      That's so awesome! Congratulations! It's so awesome to be part of your journey! :]

  • @DanNguyen-hu6pt
    @DanNguyen-hu6pt 10 років тому

    Thank you so much Mahfoud. This video is extremely helpful :)

    • @MoofUniversity
      @MoofUniversity  10 років тому

      Danny Nguyen You're very welcome! I'm glad you found it helpful! :]

  • @sanaabedouane1910
    @sanaabedouane1910 9 років тому

    thank you so much it's very helpful that's helped me to see the concept of the enzymes :D

    • @MoofUniversity
      @MoofUniversity  9 років тому

      sanaa bedouane Sweet! I'm happy to have helped! :]

  • @raissacanales6103
    @raissacanales6103 8 років тому

    what do you mean they flip over and make it easier by turning it into a line? i dont get that.

  • @drnazmy8128
    @drnazmy8128 7 років тому +1

    thank you you saved my grades i will not fail in the course i guess

  • @aishahhearts8294
    @aishahhearts8294 7 років тому

    Thank you all I say is thanks seriously with all my heart❤️🌹🌹🌹 please keep it up your great videos

    • @MoofUniversity
      @MoofUniversity  7 років тому

      You're very welcome! Thanks for watching! :]

  • @magic0chicklets393
    @magic0chicklets393 11 років тому

    this helped me a lot!

  • @fortecimo3547
    @fortecimo3547 9 років тому

    gotta love this..thanks a lot

  • @sarajs1394
    @sarajs1394 7 років тому

    I wish we have only one of you at our uni!

  • @udari111
    @udari111 10 років тому

    loved it! thanks!

  • @arinsadeghi3155
    @arinsadeghi3155 8 років тому

    Great series of videos, just as a friendly reminder that the "actual meaning of Km depends on specific aspects of reaction mechanism such as the number and relative rates of the individual steps. " Source: Lehninger Principal of Biochemistry 5th ed. page198.

  • @RomajaneAbrenio
    @RomajaneAbrenio 7 років тому

    Thank you again.

  • @abigailnizahnambeye6959
    @abigailnizahnambeye6959 4 роки тому

    Wow...thank you very much

  • @kaileyrobertson8018
    @kaileyrobertson8018 9 років тому

    Thank you so much!

  • @chetan_358
    @chetan_358 5 років тому

    How to derive derive X = Y(Sf -D×Ks/Umax-P)

  • @barakreshef5096
    @barakreshef5096 10 років тому

    your vids are great, but this particular - does not load...:(
    could u please upload it again?

    • @MoofUniversity
      @MoofUniversity  10 років тому

      I'm not sure what the problem is, but the video is loading just fine on my end. I'm sorry, but I have no idea why it's not working for you. :/ Maybe try loading it again? Or using a different browser? I dunno.

    • @barakreshef5096
      @barakreshef5096 10 років тому +1

      Ok - prbolem solved. just grabbed the time pointer to 0:02 so it started...great vids, again.

    • @MoofUniversity
      @MoofUniversity  10 років тому

      Barak Reshef Sweet! I'm happy you got it to work. Thanks, dude!

  • @merzouqhind4373
    @merzouqhind4373 4 роки тому

    thank you so much

  • @georgelagalle2298
    @georgelagalle2298 6 років тому

    Thank you so much, you made that so easy lol :)

  • @carmenbooth6350
    @carmenbooth6350 9 років тому +1

    you are awesome!!!!!

  • @japhetcatacutan9705
    @japhetcatacutan9705 4 роки тому

    THANKS!!!!

  • @rachellediab7751
    @rachellediab7751 8 років тому

    THANK YOU

  • @agdoombagdoom8540
    @agdoombagdoom8540 4 роки тому

    could not understand why km was positive?

  • @AlyssaCuriel
    @AlyssaCuriel 9 років тому

    Thanks.

  • @MOalharbi19
    @MOalharbi19 11 років тому

    thanks alot very usfel

  • @mimik9948
    @mimik9948 7 років тому

    THANK YOUUUY

  • @funsizedstoichiometry8572
    @funsizedstoichiometry8572 9 років тому

    thank you :-)

  • @darl2588
    @darl2588 10 років тому

    I love you

    • @MoofUniversity
      @MoofUniversity  10 років тому

      Da Rina Lol. Right back at ya! Thanks for the support!

  • @ifrahmalik1031
    @ifrahmalik1031 7 років тому

    Thank you
    I understood everything you said but could barely see what you wrote...I make notes so sir please try to write in a more defined manner ☺

  • @andreachiarini4685
    @andreachiarini4685 4 роки тому

    Incredibly unintuitive

  • @anupriyakp4073
    @anupriyakp4073 6 років тому

    thank you