sorry to be so offtopic but does anybody know a trick to log back into an instagram account?? I was stupid forgot the login password. I love any help you can give me
@Ben Raphael I really appreciate your reply. I got to the site thru google and I'm in the hacking process atm. Looks like it's gonna take quite some time so I will get back to you later with my results.
I am first year medical student and I just want you to know that your lectures are amazing and useful. I appreciate the way you teaching complicate lesson in easier way to understand . Big thanks
you're the onely one who alwayes saves me . you cant't imagine how much i like your videos and your way of explaining . you are my hero; i can't study without you
My brain has officially been fried from Biochem.. You did such an amazing job to explain such heavy contents. Much respect and love from Australia, thanks for saving me from failing !!
I'm a first year mbbs student ..i really like the way you explain anatomy biochem and physiology..really appreciate your work ..Thanks alot for clearing all my concepts
I wasnt looking forward to studying this topic because biochem is not my strength but then I realized Ninja Nerd made a video and now I am pumped! Thanks for making engaging and easy-to-follow videos.
Ninja Nerd Nation all the way from Perú. All I get is A's because of you. Thank God I speak english; you are legit top 10 best youtube teachers. Salute my boy
I am an old IMG with a full-time job and mother of two little girls to take care of. I finally did my Step 1 test on Jan 11. I haven't got my score yet, maybe not good enough :) It's okay. I can't thank enough to Ninja nerd lectures. Very deep insights and so well-organized, too good to be free. I particularly like neuro, cardio and bicochem, and more. My biochem UW 1st pass is 91% correct overall. I'd like to give full credit to these lectures. Thank you again for sharing these high-quality lectures!!
Km does not equal 1/2Vmax - units for KM is concentration (moles) - units for 1/2Vmax is concentration/per time Km equals "concentration [S] at which Vo=1/Vmax"
God bless you man...been running about trying to figure out this equation for like two days, you finally linked up everything in a way that makes sense
If the life would have been in my hand, you would have lived on earth for the next generation. I really don't know how I could explain my heart felt for your lecture which isn't merely this Bio(chemistry) but also the rest of the medical courses. Thanks you alot. May True Lord top up your thinking capacity.
Thanks to you doctor okey here in Algeria the place where I live we study in french language but my last solution to understand what the lessons looks like is to watch your videos 💙💙💙so thank you I appreciate your work 👏👏👏👏👏👏
thank you so much! i think there are few mistakes.. one of which is mathematical around min 9:15 when u divided the equation by k1 - you didn't divided the subtraction as well..
20:35 theorising here any experts correct me going back to the definition of Km = K-1 + K2 / K1 where Km essentially is the rate of dissociation over association a low Km = high affinity a low Km means that either the rate of association is higher and/or the rate of dissociation is lower, so more ES is formed high affinity high Km = low affinity vice versa, rate of dissociation is higher while association is lower in the context of affinity referring to how much substrate an enzyme can take on, I'm guessing that k2 is higher rather than the effects of k-1 being higher or K1 being smaller (though i thought that affinity would refer to how fast K1 would be) so low Km means higher affinity, K2 is lower, less substrate can be taken on as ES complexes take longer to form into E + P while high Km means lower affinity, K2 is higher, more substrate can be taken on as ES complexes are freed up quicker
great vid for understanding overall concept but after factoring K1 out at 8:42 you shouldn't have separated the parentheses and the left side of the equation at 9:08 is supposed to be: K1*( [ET][S] - [ES][S] ) and only that would be [ET][S] - [ES][S] after dividing by K1 as K1*( [ET][S] ) -( [ES][S] ) is basically K1*[ET][S] - [ES][S] and all of it over K1 equals [ET][S] -( [ES][S] )/K1 which is completely different case.
This is the impactt of a gud teacher ...they can make anything easier ...evrything was prct..bt can anyone tell me ..from which country he belongs to,,?????
First off, thanks A TON for making this video. It was very detailed and very helpful. However, I have an inability to understand constants. Km= (K-1 + K2)/K1 so Km is a constant but what does this constant tell us? Why do we want to know what this constant is? Where did this assumption come from? I realize that it wasn't the purpose of this video to explain that but in order to conceptualize the michaelis-menten equation and why we're deriving it, I need a top down view. Somebody, help! Thanks in advance :)
Deb, this is something that is commonly misrepresented in undergraduate courses in an effort to simplify, but Km is a kinetic constant, meaning it has less to do with binding affinity, though it can be considered analogous. I'll explain why in a second. Kd, or the dissociation constant, is a thermodynamic constant and measure of binding affinity. If we have a reaction (ES -> E+S) the Kd is equal to the rate (or you can think of it as thermodynamic favorability) of dissociation to the individual products. This would give us Kd = [E][S]/[ES]. Km, rather, is the substrate concentration when the speed (or rate) of the reaction reaches 1/2 the maximum velocity (Vmax). The reason why people often use them in the same way is because if you have a low Km, that means you require less of the substrate to reach 1/2 maximum velocity (this would indicate good binding, the enzyme is able to grip and hold onto the substrate and convert it). This would also correlate to a low Kd (meaning the rate of dissociation of the substrate from the enzyme is low, indicative of better binding). Hope that helps!
No, you are great student. You will get the topic when you understand it! It is the best habit!! I am also from those student that I can't understand from anyone. I have to study by myself deeply. You got this topic of enzymes? If no, I will make not after I understand the topic. I can send you my note about this after making it.
Thank you very much for the video. I really like your teaching style. I noticed couple mistakes or confusing points. For example, you said "V_0 = V_max/2"; I think that you meant V_0 = V_max/k_2. This mistake carried over and made the last 5 min of the video confusing for me. Thank you for your enthusiasm and passion, though. :)
I have a question! Why does steady state assumption only apply to enzymes, not to inorganic catalyst? I understood this that inorganic catalyst has rate-determining step, but enzymes don't. Why do enzymes don't have a slow step? Is it because of the structure of enzymes? It would be really helpful if you could answer me!
jh, enzymes absolutely have a rate-limiting step most of the time. It can either be the binding of the substrate (k1) or the conversion from the ES complex to E+P (k2) if we assume a classical enzyme.
Watched this video for my undergraduate biochem class and got an A+ in the class. Watching again to study for the MCAT. You’re a great teacher brother
sorry to be so offtopic but does anybody know a trick to log back into an instagram account??
I was stupid forgot the login password. I love any help you can give me
@Christopher Brennan instablaster =)
@Ben Raphael I really appreciate your reply. I got to the site thru google and I'm in the hacking process atm.
Looks like it's gonna take quite some time so I will get back to you later with my results.
@Ben Raphael It did the trick and I finally got access to my account again. I'm so happy!
Thank you so much you saved my account !
@Christopher Brennan Happy to help :)
I am first year medical student and I just want you to know that your lectures are amazing and useful. I appreciate the way you teaching complicate lesson in easier way to understand . Big thanks
Which uni?
Which uni?
you're the onely one who alwayes saves me . you cant't imagine how much i like your videos and your way of explaining . you are my hero; i can't study without you
My brain has officially been fried from Biochem.. You did such an amazing job to explain such heavy contents. Much respect and love from Australia, thanks for saving me from failing !!
I'm a first year mbbs student ..i really like the way you explain anatomy biochem and physiology..really appreciate your work ..Thanks alot for clearing all my concepts
I wasnt looking forward to studying this topic because biochem is not my strength but then I realized Ninja Nerd made a video and now I am pumped! Thanks for making engaging and easy-to-follow videos.
Ninja Nerd Nation all the way from Perú. All I get is A's because of you. Thank God I speak english; you are legit top 10 best youtube teachers. Salute my boy
This dude really said "that's a big mofo to derive" 😂😂😂 I love him for that. 👏
I am an old IMG with a full-time job and mother of two little girls to take care of. I finally did my Step 1 test on Jan 11. I haven't got my score yet, maybe not good enough :) It's okay. I can't thank enough to Ninja nerd lectures. Very deep insights and so well-organized, too good to be free. I particularly like neuro, cardio and bicochem, and more. My biochem UW 1st pass is 91% correct overall. I'd like to give full credit to these lectures. Thank you again for sharing these high-quality lectures!!
Congrats on your success! ❤️
May I ask how you're doing now? Hope you got a good score
@@dhishansivadas8227 244, not very good... Will try to make it better for CK
@@linahan1634 Lots and lots of luck to you Lina ❤❤🤞🤞
Could you please enable subtitles on this video and the other biochemistry videos? I'm deaf and would really like to watch.
You can do that urself
Just click on that CC you see on yr screen
@@GamerX-ns6sj Yes, because they enabled it. It wasn't enabled when I made this comment a year ago.
@@LostOnKorriban ok bro
This guy made me to love biochemistry and now I'm doing my Master's in biochemistry 😍
I tried reading this on my own and it felt like my head was being pounded in. Thank you so much, I understood it perfectly!
Really helpful. When my professor tried to derive it, everyone was confused lol kudos to you and thank you :)
Among all the videos in youtube regarding enzyme kinetics your one is the best, great teacher😊
It’s just like the movies we ignore the random equations in the background
And ninja nerd would be the one to focus on it other then
Watching.
Km does not equal 1/2Vmax
- units for KM is concentration (moles)
- units for 1/2Vmax is concentration/per time
Km equals "concentration [S] at which Vo=1/Vmax"
How can v max ever be half of initial velocity pls exaplin
i literally lost hope to even pass the biopharmaceutics exam...
thanks to ur videos got my hopes up ans started studying
GOD BLESS U KIND SIR❤❤
God bless you man...been running about trying to figure out this equation for like two days, you finally linked up everything in a way that makes sense
Followed this channel for my undergrad Biochem and got an A+ . Thanks
Best teacher I've seen so far. Thanks a lot sir.
Why didn’t I find your channel sooner? Thank you sooo much for explaining these concepts so clearly!!
One of the best teachers I’ve ever seen
Great 👏🏼
The best video for Michael menten equation all over the internet 😶🌫️
You're literally a life saver!! I've watched so many of your biochem videos, you are an amazing teacher!!
I just cited this in my thesis. Excellent video amazing explannation better than anything I have seen before
not me watching this 20 mins before my midterm good luck to my brain
If the life would have been in my hand, you would have lived on earth for the next generation.
I really don't know how I could explain my heart felt for your lecture which isn't merely this Bio(chemistry) but also the rest of the medical courses.
Thanks you alot.
May True Lord top up your thinking capacity.
Thanks to you doctor okey here in Algeria the place where I live we study in french language but my last solution to understand what the lessons looks like is to watch your videos 💙💙💙so thank you I appreciate your work 👏👏👏👏👏👏
this guy gonna get me through first year med
this is probably the best explanation video ive ever watched in my life
You’re literally an angel ! THANK YOU !
the massive mess up at 7:40 is supposed to be the left half = [ES] * (k1 + K2), and is fixed 2 minuts later
I don't know how to thank you you really did a great job I wish you all the best.
Great explanation, but this was a lot to grab at first, but I kept on watching it and then I can derive it anytime and anywhere
Thank you so so much. You’re an extraordinary teacher.
thank you so much!
i think there are few mistakes.. one of which is mathematical around min 9:15 when u divided the equation by k1 - you didn't divided the subtraction as well..
Guyy. Your explanations are on a whole different level 🫡🫡
Could you please add subtitles, for people from other countries who don’t know english very well(and also for me)
20:35
theorising here any experts correct me
going back to the definition of Km = K-1 + K2 / K1
where Km essentially is the rate of dissociation over association
a low Km = high affinity
a low Km means that either the rate of association is higher and/or the rate of dissociation is lower, so more ES is formed high affinity
high Km = low affinity
vice versa, rate of dissociation is higher while association is lower
in the context of affinity referring to how much substrate an enzyme can take on, I'm guessing that k2 is higher rather than the effects of k-1 being higher or K1 being smaller (though i thought that affinity would refer to how fast K1 would be)
so low Km means higher affinity, K2 is lower, less substrate can be taken on as ES complexes take longer to form into E + P
while high Km means lower affinity, K2 is higher, more substrate can be taken on as ES complexes are freed up quicker
great vid for understanding overall concept but after factoring K1 out at 8:42 you shouldn't have separated the parentheses and the left side of the equation at 9:08 is supposed to be:
K1*( [ET][S] - [ES][S] ) and only that would be [ET][S] - [ES][S] after dividing by K1
as K1*( [ET][S] ) -( [ES][S] ) is basically K1*[ET][S] - [ES][S] and all of it over K1 equals
[ET][S] -( [ES][S] )/K1 which is completely different case.
thanks, havent noticed that
By far the best explanation I've seen on this topic. Awesome
You deserve diamond ♦️ play button ❤❤
o my god it helped even after 5 years thank u so much ..very easy to understand ..
Im a master student in medicinal chemistry and im glad i came across this
If only I could hit the like button 100 times. Thank you so much.
Excellent explanation!!! Thank you very much.
Thanks for the very useful lecture and you have very nice teaching method.
That was a very good revision for biochemistry. You just helped me remember many points I forgot during my 12th class
Thanks you so much... Love from INDIA
Great video, little bit different from my book but really helped me understand the topic.
I wish this man was my professor, great explanation!
Enjoyed learning it. Thank you
this guy is a rockstar of biochemistry thumbs up for ur knowledge and presentation
Dr. Csaba Csonka sent me here 🙏❤
Thanks alot.
I got my point clear which i was trying to clear through many videos.
But your's 👍
Very helpful in clearing doubt ....love from India ❤️
Please make more videos about Genetics and medicine. I'm Health science student and you are really helping me!
this vedio was of great help...many thanks🙌
Incredible video. Thanks!
Hi I really appreciate all your lectures and well thought out explanations and illustrations. Could you make a video on vitamines?
thank you so much sir .very helpful video 🙏🙏🙏
I haven’t started watching this video, but I can tell it would be 💣🤩
This is the impactt of a gud teacher ...they can make anything easier ...evrything was prct..bt can anyone tell me ..from which country he belongs to,,?????
I am really enjoying watching you.
Thanks, this video has helped a lot.
First off, thanks A TON for making this video. It was very detailed and very helpful.
However, I have an inability to understand constants.
Km= (K-1 + K2)/K1 so Km is a constant but what does this constant tell us? Why do we want to know what this constant is? Where did this assumption come from? I realize that it wasn't the purpose of this video to explain that but in order to conceptualize the michaelis-menten equation and why we're deriving it, I need a top down view. Somebody, help!
Thanks in advance :)
I feel the same way
Deb, this is something that is commonly misrepresented in undergraduate courses in an effort to simplify, but Km is a kinetic constant, meaning it has less to do with binding affinity, though it can be considered analogous. I'll explain why in a second. Kd, or the dissociation constant, is a thermodynamic constant and measure of binding affinity. If we have a reaction (ES -> E+S) the Kd is equal to the rate (or you can think of it as thermodynamic favorability) of dissociation to the individual products. This would give us Kd = [E][S]/[ES]. Km, rather, is the substrate concentration when the speed (or rate) of the reaction reaches 1/2 the maximum velocity (Vmax). The reason why people often use them in the same way is because if you have a low Km, that means you require less of the substrate to reach 1/2 maximum velocity (this would indicate good binding, the enzyme is able to grip and hold onto the substrate and convert it). This would also correlate to a low Kd (meaning the rate of dissociation of the substrate from the enzyme is low, indicative of better binding). Hope that helps!
Thank you very much Sir... amazing job to explain it ....
I WATCHED THIS VIDEO AND I LIKED IT VERY VERY MUCH THANK YOU
Great ...when I got it at 8:34....I got shocked but the teacher explained it imadiately so again I got super
hihi! i really find this difficult, maybe I am just a really bad student but I think it is soooo hard! at least i tried to understand :(
No buddy u r enough! Just try to read it on your own and just forget about the world! :)
No, you are great student. You will get the topic when you understand it! It is the best habit!! I am also from those student that I can't understand from anyone. I have to study by myself deeply.
You got this topic of enzymes?
If no, I will make not after I understand the topic. I can send you my note about this after making it.
Love you man!!!! You are the best!
you would be the main reason why i would ace biochem
Explained really well. thank u sir
Need it for my Pharmacokinetics finals tomorrow
This mathematical equations goes out of my level 😂
I didn’t get why the enzyme having higher substrate affinity can not take higher amounts of substrates?
Because they are more exclusive for those substrates they have affinity for
Thanks sir you are great 😍❤
Great explanation🎉
I'm a bit confused about the end...how is an enzyme with LOW substrate affinity able to take up MORE substrate??
My question is why i need this for medical course enzyme kinetics my brain is overwhelmed
Thankyou for your efforts sir these videos are very very helpful
Love from tamil nadu
I didn't understand a thing actually. Maybe there are some previous skills i'm lacking off because i payed 100% attention to your explanation.
I don’t have much math background except for statistic and I understand everything you said o…..9
Please, can you insert an automatic translation?
It is much easier for me to understand when the Serbian translation is included.
Super helpful! However, how would we find the Vmax without using the Lineweaver equation or plot?
lol love that he factors out K1, and one minutes later undoes it
Thanks for going through the steps. Literally every explanation I have seen assumes the steps are obvious
This the only video i havnt understa d
Thank you so much. Great job 👍
You are amazing thank you for a great explanation
THIS VIDEO IS FUCKING SICK THANK YOU
You deserve the world and everything in it, thank you so much🤍
We could never repay you, never!!!
May all your wishes come true!
Thanks 😎
Thank you very much for the video. I really like your teaching style. I noticed couple mistakes or confusing points. For example, you said "V_0 = V_max/2"; I think that you meant V_0 = V_max/k_2. This mistake carried over and made the last 5 min of the video confusing for me. Thank you for your enthusiasm and passion, though. :)
Exactly my point how can vmax be half of v0
How did u understand it as vmax / k2
Thank you a lot. You made me understood the Michaelis Menten equation with ease
I have a question! Why does steady state assumption only apply to enzymes, not to inorganic catalyst?
I understood this that inorganic catalyst has rate-determining step, but enzymes don't.
Why do enzymes don't have a slow step? Is it because of the structure of enzymes?
It would be really helpful if you could answer me!
jh, enzymes absolutely have a rate-limiting step most of the time. It can either be the binding of the substrate (k1) or the conversion from the ES complex to E+P (k2) if we assume a classical enzyme.
man you supper great
thanks so much for this. i got to subscribe before even finishing the video
it is super lecture i appreciate you fantastic
THANK YOU THANK YOU THANK YOU