I dont know if you see this, Your explanations are super clear unfortunately the website is off. What happened to the course? are you planning to upload new videos here?
Hi, I tried to run fastqc after installed java and it said " Assistive technology not found: or g.GNOME.Accessibility.AktWrapper..." What should I do in this case?
Hi first of all I would like to thank you for this series of ngs analysis UA-cam and I would like to ask you why we use these kind of program such as ubuntu or others comment line platforms which are not easy to follow while there are ready programs such as galaxy and genius that are ready to put your data and get the result in one click. Your answer will be very appreciate Regards Adnan
Hey, thank you for your comment! Web applications such as galaxy are very nice for small analysis. But by learning how to use software like this you will be able to: 1. Do more customized analysis 2. Streamline your analysis via a pipeline. This means, you can write a script that will do very complicated analysis of, lets say, 100 files that each include 500k reads with one click. This is something not really possible with web application, where even uploading one file with 500k reads takes some time. 3. There are more software applications and far more detailed settings with tools like this than you will have with web applications. I hope that helps!
hi im really greatful for the tutorial as i am trying to learn the basics. I tried downloading the java to read fastqc but it didnt work . i could send a picture of the response i got if possible . I would appreciate any answer
hello, I can't successfully install FastQC because it requires java stuff. Is this java needs to be installed separately or is it already included in the FastQC file when downloading it in Babraham?
Hi Anu, I am doing my masters degree thesis on whole genome sequencing, did you ever find out how to do it? I would appreciate if you could consider helping me, thank you.
when I put the command ./fastqc , so I got this error Exception in thread "main" java.awt.AWTError: Assistive Technology not found.... please give me some solution.
Hey, it's my first time seriously codding and I'm having trouble with working the terminal. I'm using Windows power shell and I don't know which commands should bring me to the same place you are. Like how do you open your terminal to sequence@sequence.... I'm confused please help.
plz help, I am getting this error when downloading fastqc - exception in thread main java.awt.AWTError: assistive technology not found: org.GNOME.accessibility.AKTWrapper
hello,It is very useful for me.but I have a question, when I run it on bash on ubuntu on windows,it can not give me the error exception in thread "main" java.awt.HeadlessException: No X11 DISPLAY variable was set, but this program performed an operation which requires it.do u know how to solve it, thank u.
Nice work, I'm waiting for your course about pipelines!
did he ever get to the video about pipelines???
thank you, your explanation was simple concentrated and clear even for my francophone background.
I dont know if you see this, Your explanations are super clear unfortunately the website is off. What happened to the course? are you planning to upload new videos here?
Thank you so much. Your explanation was so helpful.
many thanks for such a practical tutorial
thanks you, classes..
Nice course, salute Peruvian.
Hi man, great videos thank you. Your website is down, is there a way of accessing your course and additional videos somehow? Cheers
Hi, I tried to run fastqc after installed java and it said " Assistive technology not found: or g.GNOME.Accessibility.AktWrapper..." What should I do in this case?
Same problem
This video is a great help for me thank u so much
Please how can i get the other videos for trimming of adapter,Indexing,aligning .
Hi first of all I would like to thank you for this series of ngs analysis UA-cam and I would like to ask you why we use these kind of program such as ubuntu or others comment line platforms which are not easy to follow while there are ready programs such as galaxy and genius that are ready to put your data and get the result in one click. Your answer will be very appreciate Regards Adnan
Hey, thank you for your comment! Web applications such as galaxy are very nice for small analysis. But by learning how to use software like this you will be able to:
1. Do more customized analysis
2. Streamline your analysis via a pipeline. This means, you can write a script that will do very complicated analysis of, lets say, 100 files that each include 500k reads with one click. This is something not really possible with web application, where even uploading one file with 500k reads takes some time.
3. There are more software applications and far more detailed settings with tools like this than you will have with web applications.
I hope that helps!
Hi Thank you so much for your replying I wish you all the best. Adnan
Thank you. Very nice and simple
hi im really greatful for the tutorial as i am trying to learn the basics. I tried downloading the java to read fastqc but it didnt work . i could send a picture of the response i got if possible . I would appreciate any answer
hello , these video's are very simple easy to lean , thanks for the video's . are you providing any course at current
Hi Your website is now linked to random ads. How can we access the course you referenced to?
Muchas gracias, me ayudo mucho, saludos desde Colombia
hello, I can't successfully install FastQC because it requires java stuff. Is this java needs to be installed separately or is it already included in the FastQC file when downloading it in Babraham?
Hello, you have to separately install java like at 04:11 and I think you could try default-jre if the openjdk-8-jre do not work
@@marbleous Thank you so much...... It really helped me...
life savior:))
tnx alooooooottttttt
what if we want to analyze a whole genome sequence, from where we can get sra no. ? or can you refer any reading or suggestions
Hi Anu, I am doing my masters degree thesis on whole genome sequencing, did you ever find out how to do it? I would appreciate if you could consider helping me, thank you.
when I put the command ./fastqc , so I got this error
Exception in thread "main" java.awt.AWTError: Assistive Technology not found....
please give me some solution.
same problem here
Did you find the solution? I am getting the same error now
@@kousalyadevi5209 SAME PROBLEM HERE
Same as you.
Hey, it's my first time seriously codding and I'm having trouble with working the terminal. I'm using Windows power shell and I don't know which commands should bring me to the same place you are. Like how do you open your terminal to sequence@sequence.... I'm confused please help.
I am on same problem as you let me know if you got over it..!!! thanks in advance
please provide a video based on the installation of an 11.9 version. I am trying for a long time but unable to do it. please help
Well it works perfectly for me, make sure of the last java update and after that try again.!
Please how do I access the course on nextgenerationsequencinghq.com
hi could you access the course? I am also looking for it kindly lemme know if you find.Thank you!
Hi, your website is not working. Please help!
plz help, I am getting this error when downloading fastqc - exception in thread main java.awt.AWTError: assistive technology not found: org.GNOME.accessibility.AKTWrapper
this error can be removed by uninstalling the headless version and installing java without head
@@lalitbhargava7305 thnx...it helped
hello,It is very useful for me.but I have a question, when I run it on bash on ubuntu on windows,it can not give me the error exception in thread "main" java.awt.HeadlessException:
No X11 DISPLAY variable was set, but this program performed an operation which requires it.do u know how to solve it, thank u.
Im sorry, I havent encountered the problem and a google search didnt help me. Are you running it on virtual machine?
No, I am using Windows bash, it seems that I didn't a graphic windows, when I install xplay, it solved. Thanks bery much for your help and videos.
@@zhaokenny2721 Hi Zhao, could you please tell me how did you solve this problem? Thanks in advanced.
Bitte, bitte HERRR!!! danke schon!
very good ,thank you
assistive technology error while execution of fastqc,,,, plzz guide
please help me if you find the solution
I would prefer going through the reports rather than how to tun the software
Waoooo
Thank you so much.