Headspace gas chromatography
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- Опубліковано 1 лип 2024
- If the analytes are very volatile, they can be sampled, injected and then separated as gases. One of the techniques for this is headspace GC. This technique always uses an internal standard.
Look at this video: • statistics and drunken...
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Really thank you sir, for talking about your own experiences ! I think these arguments are better than theory sometimes !
+Alessio Giovinazzo Thanks for the comment. Sometimes I think I can only learn as a result of self exploration. This is a little humiliating as an educator.
I think Einstein knew the answer.
"Theory is when you know everything but nothing works. Practice is when everything works but no one knows why. In every case, theory and practice are combined: nothing works and no one knows why"
Qué bien está la explicación. Hablas claramente y se traduce muy bien. Gracias. Saludos desde México.
Thanks a lot for this video!
I´m currently working on a school project about forensic chemistry, and this helped me quite a lot. (Blood alkohol analysis). A shame that there is nothing to find in my native language (german).
Phenomenal video thank you!
thanks
Quick comment. This is, of course, a gas chromatography application. The GC run time is short, less than five minutes for ethanol. I do not think that there was a temperature ramp.
If I was facing this I would find a reference and follow the reference. A general answer would be to make a standard at 2000ppm, mixed with all four compounds. Or buy one already made. And use the internal standard specified by the reference. Juliet, I guess the short answer is to ask someone else.
When preparing sample aliquots, do you need to filter them after blending before putting it into the test tube and heating? I would think small particles would not matter since gas is taken instead of solution. Please advise
Upon rereading your comment, it occurs to me that you may not want to blend the samples. The volatiles will leave the sample during the blending step. Think about what you are looking for and look at its physical characteristics, (boiling point) You do not need a big sample; 100-1000ul...............
Great explaination
thanks!
I make videos on areas that are not flooded with videos. For differential equations, go to KahnAcademy. He does a great job.
Thanks for the comment........
Dear mrphysh! Can I ask you about headspace? If I have one vial with headspace 4 ml (total volume 20 ml) and second vial with headspace 4 ml (total volume 200 ml) - will concentrations in 2nd vial headspace be 10 times more than in 1st vial headspace? Thank you
What are you trying to achieve. what is your objective? Your question makes no sense and is of no interest to me.
@@mrphysh thanks for your answer. I just want to increase gases concentrations.
hello, how to do calibration if the sample is on gas?and how much volume that we need to inject manually to the gc FId HP5. and how about the method if our sample is on gas not liquid?
watch the video over and over. It will be worth your time.
@Mrphysh, can you tell me how l can prepare my stock, am analysing BTEX in water?
study the video
De nada
Trust your intuition. I assume that you are doing this manually. Be aware of carry-over with the syringe.
Can it be used for quantitative analysis?
Yes. The foundation for analysis is always "keep everything the same" That way the response for the standards can be used to calculate the unknown.
Thankyou sir
The GC run is only about 5 minutes. There is no temperature ramp; ... no cool down delay.
go around and around it in your head. You need to really understand it. The whole idea of analysis is that everything (but one) is the same, standard to control to sample.
Sir why dose headspace