This was so relaxing compared to day-to-day blood banking! Nice to see someone working without stress, quietly, without mounds of paper on the bench and a phone ringing off the hook. :)
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Literally having a transfusion now. I was waiting for ages for my bag and they told me my cross match failed the first time. So now I’m here to see what they meant. Plus I’m bored!
Awesome! I went back to my middle school for a STEM career day (they LOVED IT) and told em all to go to WVC! I'm challenging the MLS boards in a year now, re-studying everything, and your videos are a great help. Have you also seen www.pathologystudent.com/ ? I like her explanation of G6PD deficiency, among other things: www.pathologystudent.com/?p=1184I'm actually hoping to continue on and get a Master's in Clinical Lab Science (online), depending on how expensive it is...I'm so glad that Wenatchee is being nimble about an MLS program!! I will keep spreading the word about WVC! Hope all is well, say hi to Katie for me!!
Nice to see the procedure but it would have been better if you had explained why you did the washes and why you added LISS and why you added AHG and then Check cells ... just to make it more complete in case someone watching was still confused with the reasoning behind each step.
I probably made the assumption that if someone is watching my videos, they would have watched the videos focusing on the antibody screen and antibody panel first. I explain all of this in those videos.
This would be a potentiator like low-ionic-strength saline (LISS) or polyethylene glycol (PEG). LISS is the most common. Other potentiators are enzymes, but those are used for more specific testing.
I have a crucifix with a little bit of red paint I want to put more red paint on it the nails are painted silver it's not real nails I'm curious what paint do I need to use
Hi Val, every facility determines its own standard operating procedures (SOPs). Those SOPs must be a balance of patient care, workload and cost. Performing an AHG crossmatch on every unit for every patient would be the safest, but taking into consideration workload and cost, a facility may accept the risk associated with not doing the AHG crossmatch.
I probably made the assumption that if someone is watching my videos, they would have watched the videos focusing on the antibody screen and antibody panel first. I explain all of this in those videos.
What is that add in first step and centrifuge for 15sec .and what add in second step that called it or something before you go for some time and thin add AHG finally what that add after AHG Plz explain Thanks from Yemen 🇾🇪 in Arabic countries
Hi Rahel,I do not do videos for gel cards. However, whether you are using tubes or cards, the usual step after a positive antibody screen is an antibody identification panel.
hi med tech student here is ok to resuspend that way? my lecturer kicks a fit if I do it that way she says it's over resuspending I'm genuinely asking. thank u
Hi Rochelle what does "that way" mean exactly? I was always taught to not tap the tubes as that can damage the button. I have never had anybody tell me that my method is too strong.
Hi Douglas, Check cells (also called Coombs check cells) are reagent red cells that are added to the tubes that are negative after the addition of the anti-human globulin in the direct and indirect-antibody tests. They verify that the negative tubes are true negatives, not false negatives.
I can't tell you what to do or not do. If you are following a procedure, then it should instruct you what the acceptable specimens are.Currently plasma is the most common specimen type for blood banking, but in the past serum was widely used. For tube testing with plasma, we just look at agglutination and background to determine reaction strength. For tube testing with serum, we also look at agglutination and background to determine reaction strength, but we also have to consider hemolysis as a positive reaction. Serum has the ability to activate complement and therefore we may see RBC destruction in the form of hemolysis.
The way u shake the tube when I do it that way she says that it would break up fine agglutination very good video BTW very helpful my finals are in a few day
This was so relaxing compared to day-to-day blood banking! Nice to see someone working without stress, quietly, without mounds of paper on the bench and a phone ringing off the hook. :)
Being a teacher isn't always relaxing, but making these videos was. :)
He is not in Saudi Arabia thats why 🤣
what does that even mean@@frontlinersaudi8076
I am a level three laboratory student in university of RWANDA .This video is going to help to improve my hematology practice.
Thank you! I just refresh my knowledge in BB because it’s my first day of internship tomorrow and BB is my first post 😊
I hope it helped.
you boy vt to 😭Tvg😭gvgvgvgvgvgtttv vv vgvg goodv tv vgoodvv😭gvvv😭😭vvvvvbyee😭v😭g😭 vv vvvtevgvgr vvvv😭😭 vgvvvvvg t by ttvgvtv by vggggegevgvgggvgvgvvvvgrvrtvvgvvGggg vv 😭😭 vvvvgvtgvGggg ggvg vvgvv vv vv tg😭vvvvgg by😭 vv
Its my first day internship today and bb is my first post too 🤭😊😊
Hi @@joannepiwladan8135BB is my first post too!
Tomorrow is my 1st lab Immediate spin Crossmach and I know what to do now. Thank you. You are best of the best teacher.
soooooooooooo how is life ? 0_0
Hi there, Medical Technologist here, nice to watch your video specially for like me handling Chemistry section only. Nice to refresh the brain cells.
Literally having a transfusion now. I was waiting for ages for my bag and they told me my cross match failed the first time. So now I’m here to see what they meant. Plus I’m bored!
Miss Alima, I will let your healthcare providers answer the question about why your crossmatch failed. I do not make comments in situations like this.
thank you , tomorrow my blod bank practical exam , the video helps me
Ive got my first crossmatching procedure tomorrow and i was really nervous but now im confident. this was very helpful! thank you!
Thank you and good luck.
Im actually nostalgic over the particular sound of those centrifuges running😂
We are talking about starting an MLS program, so maybe you will get the chance to come back. Wouldn't that be fun.
Awesome! I went back to my middle school for a STEM career day (they LOVED IT) and told em all to go to WVC! I'm challenging the MLS boards in a year now, re-studying everything, and your videos are a great help. Have you also seen www.pathologystudent.com/ ? I like her explanation of G6PD deficiency, among other things: www.pathologystudent.com/?p=1184I'm actually hoping to continue on and get a Master's in Clinical Lab Science (online), depending on how expensive it is...I'm so glad that Wenatchee is being nimble about an MLS program!! I will keep spreading the word about WVC! Hope all is well, say hi to Katie for me!!
Sir our college don't have apparatus for this crossmatch .. So thank you for making video for student like us ❤️
You are welcome. I am glad it was helpful.
Nice to see the procedure but it would have been better if you had explained why you did the washes and why you added LISS and why you added AHG and then Check cells ... just to make it more complete in case someone watching was still confused with the reasoning behind each step.
I probably made the assumption that if someone is watching my videos, they would have watched the videos focusing on the antibody screen and antibody panel first. I explain all of this in those videos.
Hello! I want to ask the following: in an indirect antiglobulin test, what exactly is included in the first bottle that you drip before incubation?
This would be a potentiator like low-ionic-strength saline (LISS) or polyethylene glycol (PEG). LISS is the most common. Other potentiators are enzymes, but those are used for more specific testing.
@@patricktracy9947 thanks! we use liss
@@patricktracy9947thank you so much ❤❤very helpful
thank u so much for making these videos🙏🏻
Hi Samana I am glad they are helpful for you.
Great explanation, easy and clear thank you Patrick.
Can we discard the superanant of every step before performing next step
Yes
Without medical officer blood issunig to patient wrong ya right
You are the best, thank you so much.
Look at all the views youre getting! I knew youd be a youtube superstar
Superstar...hmmm, I don't know about that. It's fun though.
ptracy@wvc.edu
I lived in Saudi Arabia for five years. I taught at a university in Dhahran.
Patrick Tracy impressive, I wish if you were my mentor in the international medical center in jeddah saudi Arabia
well done sir
Patrick Tracy welcome and i hope that u r comfortable here in KSA 💚
Funny that I am actually watching this from Dhahran. Very good explanation.. Thank you :)
I have a crucifix with a little bit of red paint I want to put more red paint on it the nails are painted silver it's not real nails I'm curious what paint do I need to use
ok I'll check that out thank you for your feedback.
Hi is there any benefit in doing an AHG XM on a patient with a negative ABSC and no hx of transfusion or antibodies?
Hi Val, every facility determines its own standard operating procedures (SOPs). Those SOPs must be a balance of patient care, workload and cost. Performing an AHG crossmatch on every unit for every patient would be the safest, but taking into consideration workload and cost, a facility may accept the risk associated with not doing the AHG crossmatch.
A few sentences at every stage explaining what is actually being tested and why would have made this video perfect.
I probably made the assumption that if someone is watching my videos, they would have watched the videos focusing on the antibody screen and antibody panel first. I explain all of this in those videos.
I totally agree
What is that add in first step and centrifuge for 15sec .and what add in second step that called it or something before you go for some time and thin add AHG finally what that add after AHG
Plz explain
Thanks from Yemen 🇾🇪 in Arabic countries
Thank you👍😊
You're welcome.
What temp is the cell block used?
35-37 Celcius
How are you getting plasma from a Red top vacutainer?
Do I say in the video that I am using a red-top tube? All of my videos use an EDTA tube thus I am working with plasma.
How many cc of blood specimen is needed per unit of blood?
If it is a unit of whole blood, probably 450 ml. If it is a unit of packed RBCs, probably 300 ml.
Please what's cc?
@@thernky7677 centimeter cubic
For IAT what you use here ? Is that LISS?
Yes, that is LISS.
@@patricktracy9947 thank you ❤️ from india
For how much time centrifugation is done ?
For washing RBCs, it is 60 seconds. For grading tubes, it is 15 seconds. Both are done at 3400-3500 RPMs.
correct for: bless
Thanks for the video 👍🏼
so helpful!!! ❤️
No microscope confirmation?
Microscopic confirmation is not always required. It depends on the standard operating procedure (SOP) of the lab.
Sir ..what’s the meaning IAT?
Indirect Antibody Test
I'm new to blood banking. I need somebody to explain it to me using gel card, what are the steps you take after the antibody screen test is positive
Hi Rahel,I do not do videos for gel cards. However, whether you are using tubes or cards, the usual step after a positive antibody screen is an antibody identification panel.
hi med tech student here is ok to resuspend that way? my lecturer kicks a fit if I do it that way she says it's over resuspending I'm genuinely asking.
thank u
Hi Rochelle what does "that way" mean exactly? I was always taught to not tap the tubes as that can damage the button. I have never had anybody tell me that my method is too strong.
Sir cross match and compatible lable without medical signature blood issue to any body yes or no
It takes 30 seconds for the machine to open
smooth AF
اللي عندهم اختبار عند عبد الحفيظ.. بالتوفيق ياقلبي
Searching for blood bank job.5 years of experience in blood bank field
Please what is check cell
Hi Douglas,
Check cells (also called Coombs check cells) are reagent red cells that are added to the tubes that are negative after the addition of the anti-human globulin in the direct and indirect-antibody tests. They verify that the negative tubes are true negatives, not false negatives.
seems like not much explanation
How can you obtain plasma in a red tube?
Red-top tubes are for serum, so I don't know how you would get plasma from one.
Is it ok to use serum instead of plasma for the procedure?
I can't tell you what to do or not do. If you are following a procedure, then it should instruct you what the acceptable specimens are.Currently plasma is the most common specimen type for blood banking, but in the past serum was widely used. For tube testing with plasma, we just look at agglutination and background to determine reaction strength. For tube testing with serum, we also look at agglutination and background to determine reaction strength, but we also have to consider hemolysis as a positive reaction. Serum has the ability to activate complement and therefore we may see RBC destruction in the form of hemolysis.
Patrick Tracy thank you for responding, really helpful!!
What is IAT?
Hi Luqman...the IAT is the Indirect Antibody Test.
Thank you sir
You're welcome.
Great vid can you make one on antigen typing
Hi Frank,I don't have any plans to make antigen-typing videos, but maybe if I have time this fall I will do it. I am glad you liked the video.Pat
I can't understand this video 😑
👍🙏🙏great
I am glad it was helpful.
thank you place can you give me your email because Im student from saudia Arabia
The way u shake the tube when I do it that way she says that it would break up fine agglutination
very good video BTW very helpful my finals are in a few day
I think I address that in my agglutination reaction video. ua-cam.com/video/SSzcJRGwa3g/v-deo.html
CovidImages need to be invested more than half19
I don't understand your comment.
This video is not helpful at all 😭
can I have your email please
ptracy@wvc.edu