Cross match test major and minor

[@HumanoidRobo]

Crisp and clear ... Could you provide the rationale for the each and every step why it is done from the next video ( atleast ) so that we need not read it multiple times. Thanks for such an amazing video.I'm a vet too 🙂.

[@barathanpugazendhi8779]

Brief explanation of major and minor matching. Thanks.

[@mehregankbi]

as part of my work i regularly run indirect coombs tests for pregnant women Rh+ ab screening. i have never seen anything close to the hemagglutinations you showed. but i rarely see only one small agglutination on the whole slide and according to my education, that's not enough to call it positive.
also, i have never seen the technique of mixing the button at the bottom being mixed with a micropipette. instead we shake the tube while examining it under the light. if it's macroscopically negative, then we put lamellas (slide cover slips) over it so as to avoid roleaux formation.

[@twiringiyimanaemmanuel9742]

A very nice video . But i think we have to test for ABO blood group first.

[@amylwin1271]

Thank you so much for crystal clear explanation.

[@gaguashutosh]

Beautifullly explained 😊

[@byvinodkhanagwal2106]

Thanks very much

[@magimaidass.a4667]

Help full video thanks for this video

[@mahimachaubey1290]

Thanks for given useful knowledge mam

[@dranandbadwe4357]

Very good & clear presentation

[@Pathologymadesimple]

Thankyou sir

[@kkm143.]

Brief explanation of major & minarets matching Thanks you so much

[@sahdeopandit8384]

Mai laboratory technologist hu mujhe ek cheej badi psnd aaae aapki....o hai last wali smile....👏

[@MandeepKumar-hp3kv]

Good JOB 👍👍🎉🎉

[@PrakashPrakash-um7te]

Thanks mam for your these video to my updates knowledge and cleared my doubts.

[@Pathologymadesimple]

Prakash Prakash welcome 🙏🏾

[@sangeetarajputrajput5235]

Thanks u mam
A very nice video

[@Abhisheksharma-bj1wy]

Thank you for such a good description

[@ISMAILBASHA_9181]

Just add procedure in this video disruptions..

[@janakimgrmgr4078]

#@@#@@##!​@@ISMAILBASHA_9181

[@dhruvpatel3468]

Excellent explained.
Thanks a lot 🫡

[@eshcolgaanadhyaanaalu4518]

Thank you so much madam

[@medcrumbs]

Thank you 🤗

[@birthpatel3970]

Its my humble request to make a video on different blood groups in different species of animal's.

[@Pathologymadesimple]

Excellent suggestion

[@krmanjahmad659]

thank you so much

[@yuvaraja.s4228]

Thanks for your explain very use full video

[@asneelasheshlal6485]

Can I use plasma for BUN, Gaba, NPY, CCK, SOD, GSH-PX deterimnation in dairy goats. I have read papers where serum is used but my advisor saying to use plasma, please advise.

[@SporshoMedia]

excellently explained. thanks a lot

[@carle5538]

Very nicely explained. Thank you.

[@vijaydevarakonda8079]

Nice explanation mam... Thankyou very much

[@sanjaytiwari4202]

Your presentation is quite gentil and professional i like it
Wishing you a very happy new year 2 o 2 3 with family dear madam

[@Pathologymadesimple]

Thank you very much ❤️❤️❤️

[@mangeshjangra5971]

Very helpful video thank you

[@KAUSHIKPATHLAB]

described very clearly

[@Pathologymadesimple]

Thankyou

[@sudharamasudha9971]

thank you so much very nice explained.

[@mkpathology4991]

Enjoyed watching your video and learned a lot

[@mkpathology4991]

How many procedures do cross matches do?

[@jyotishmanmedhi7804]

great explanation. thank u

[@Pathologymadesimple]

Welcome 😍

[@hrchaudhary8075]

Thanku mam

[@havishapanchal6339]

Thanks it was wonderful 😊

[@bupemusumali75]

I like the video doc...
Any video on blood indices

[@KAUSHIKPATHLAB]

thanks mam

[@dineshramesh8390]

Thank you very useful video

[@aannajarvis4188]

Very nicely explained, thank you!
BTW, is it rouleaux formation normal in dogs?

[@Pathologymadesimple]

Annajarvis Pao
If our canine patient has high proteins / acute phase proteins / on going inflammation then rouleaux formation is profoundly seen

[@Pathologymadesimple]

Annajarvis Pao
If you want to differentiate rouleaux from agglutination then add a drop of normal saline
If erythrocytes seperate then it is rouleaux
If they still appear clumped then it is agglutination.
Agglutination showed there are bound antibodies on the erythrocytes surface.

[@aannajarvis4188]

Doc, what about in equine?

[@Pathologymadesimple]

Annajarvis Pao For equines use Alvedia blood typing and cross matching kits.

[@gordyjohnson8931]

Very helpful. Thank you.

[@Pathologymadesimple]

Welcome

[@Darling_Rishwik]

Thank u mam

[@varangantisandeep9609]

Very nice explanation 👌

[@drprabhatkumar8213]

Great 👍👍... Keep it up

[@ravikantsahu4169]

EDTA vial me serum kha se aayga vo to red vial m ayga n

[@Pathologymadesimple]

Edta vial - plasma
Did I say it wrongly?

[@pateljay2363]

Thank You..Need it..😊👍

[@Masimo275]

Great video, thanks!

[@Pathologymadesimple]

Your welcome

[@khalid-hasan.]

Thanks mam !!!

[@monimonika6145]

Tq ❤️

[@emweemagray2812]

Goo job

[@hemuhemat1498]

Really nice video

[@ramsharma1272]

Nyc

[@braytonkihanga5714]

very good we understand you're presence

[@alter_gwii]

Thanks for this.
I couldn't hear what she said at 1:33 clearly though.

[@alter_gwii]

I couldn't hear what she said at 3:07 too.

[@praveenkumar-cs1ry]

Amazing Explanation...👌👌👌

[@Pathologymadesimple]

praveen kumar Thankyou!

[@vihaanmore1557]

Nice video for cross matching

[@doaaosama4516]

Thank u very much 😊

[@keshavap1884]

Hi akka Thanks for information akka

[@arnavswarnkar9265]

Thank you

[@meshramshiva1119]

Like it ❤️

[@Ranjitapoudel]

Thanks ❤

[@arslanparamedics5950]

Excellent mam

[@kittycat205]

Very useful video mam

[@mohammedidreesjazm3335]

What happen if I added AHG reagent before microscopic examination??and what the differences between minor,major cross matching?

[@Pathologymadesimple]

Major is Donor blood and recipient serum, ( helps to know if recipient has pre existing antibodies against donor blood type)
Minor is recipient blood and donor serum ( here we ll know if donor has pre existing antibodies or complements are present against recipient blood ).
Both should be not have agglutination

[@ashishasari4394]

Great information

[@Pathologymadesimple]

Thankyou

[@Hijazibhatti]

really helpful for me.
im a science teacher.

[@santhu423santhu6]

Superbbbbbbbbbbbb darling

[@santhu423santhu6]

Tq youuuuuuuuuuu for loveing my coment

[@santhu423santhu6]

Good night swt dream

[@vairamuttudassan]

Nice

[@ayenmedia87]

thanks alot

[@mohanreddybussu318]

Explain it in AHG and albumin method also

[@bikramadityakumar3612]

Nice video

[@tamangsanu2012]

nice

[@swatintertiment]

very Nice

[@boyanarsimlu7730]

Nice video

[@boyanarsimlu7730]

normal saline and rbc 3 times washing how much centrifuge rpm

[@sabrinacovey6322]

Why is the rinsing step necessary? Can results be trusted if the rinsing steps are skipped?

[@Pathologymadesimple]

Sabrina Covey Can’t be
Washing RBcs is very very important

[@Pathologymadesimple]

Without washing RBCs you will see unnecessary clumping that will interfere with true agglutination

[@freeFire-wc1wu]

good

[@rajankumar-ji8zs]

Please mention the reference book from which you have made this video.

[@Pathologymadesimple]

Kirk and bistners handbook of veterinary procedures and emergency treatment

[@muhammadikram387]

You are very beautiful 😍 last seen bye

[@alter_gwii]

When you said microliter, did you actually mean milliliter?

[@Pathologymadesimple]

ml

[@Pathologymadesimple]

Microliter 😀

[@technician5470]

Your video is good.

[@Pathologymadesimple]

BHUSHAN KUMAR thankyou so much

[@vihaanmore1557]

So nice your testing method majar n minor

[@shahrukhshaikh9055]

Mam plz make cross matching videos with use of jel card

[@Apratim98]

Hey! I wanted to ask you about human blood bank system in India if you any idea.. so in India after ABO and RhD typing of the patient blood do we do antibody screening of we directly go for cross matching?

[@Pathologymadesimple]

They do both actually

[@Apratim98]

@@Pathologymadesimple oh i see! Thank you😀

[@habiburrahman412]

Donor RBC with recipient serum. As well as Recipient RBC with donor serum But u show Donor RBC with donor serum,???????

[@Pathologymadesimple]

That is donor and recipient control, to check autoagglutination.

[@Pathologymadesimple]

If you watch the video fully, I would have incubated 4 tubes, 2 of them are major and minor, other two are donor and recipient controls to check per existing agglutination.

[@soundsofheaven1542]

Nice video, I just got confused how we can differentiate rouleaux to agglutination, sometime I am doing this test I get so confused if I am really seeing rouleaux

[@Pathologymadesimple]

vinicius prado de oliveira Is you see stack of coin like appearance then it is rouleaux. Agglutination is an irregular aggregation of RBCs which will appear like the one I showed in video.

[@soundsofheaven1542]

Varsha Scooby and like you said I can also add one more normal saline drop if I still have some doubt? If it is rouleaux it may disappear if it is not rouleaux it will keep toetther right?

[@Pathologymadesimple]

vinicius prado de oliveira exactly

[@danhlethanh6126]

After wash RWC, why do you added RBC donor to donor serum?

[@Pathologymadesimple]

danh le thanh To rule out auto agglutination in donor blood and also acts as negative control

[@Alimac_Otsab]

This is such a great video, thank you very much.

[@Pathologymadesimple]

Thankyou! Welcome 😊😊😊

[@pubgkr9350]

It is plasma or serum?🙄

[@Pathologymadesimple]

Serum

[@kishoreparida7892]

Mam love you

[@nandanikaur2401]

Mam..plz tell me that ...Is major minor test necessary for primary blood transfusion in dogs? Is there any risk of primary blood transfusion without do any major minor test in dogs..??
Plz tell me...it's very urgent for me to know that. ..

[@Pathologymadesimple]

Nandani Kaur
First time blood transfusion without cross matching is fine if it is emergency
But best practise is always do typing or transfuse DEA 1 negative blood.
Second transfusions 100% require both cross matching and blood typing test. Because if blood doesn’t match the reaction can be fatal.
Better to do properly 😊

[@ashishverma5116]

Give more video on microbiology topics.

[@Pathologymadesimple]

Can you tell me what topics should I cover?

[@dineshramesh8390]

Please send tail cross matching procedures

[@mltknowledge4534]

I love you mam😁😀

[@kawcherahmed1547]

I love you you looking nice

[@hayderbioideascraft9396]

Nice 👍

[@bokkakanakaprasad3053]

In this video not running ordering, missing some clips

[@asgstunnervlogs5233]

Hindi

[@arulananthanarul7683]

Super

[@sairam-tu2ro]

Similarly is pronounced as THIMILARLY 😀😃😄😁😆

[@Pathologymadesimple]

😂😂😂😂😂 lol I know I couldn’t stop laughing

[@mdsajumia187]

200 Serum + 200cell suspension tell please

[@Pathologymadesimple]

200 micro litre serum and 200 micro litre washed RBCs

[@arulananthanarul7683]

I like you video

[@Pathologymadesimple]

Arulananthan Arul Nandri 🙏🏾😊

[@arulananthanarul7683]

Neenga Doctor raa Ella lab techniciance naa sollunga

[@arulananthanarul7683]

Good night bye.

[@arulananthanarul7683]

Neenga Doctor tha OK vaa

[@arulananthanarul7683]

Enakku Have A nice day

[@BaThienFlute]

Come back pls

[@abhijitbhaumik8306]

How to do Crossmatch of neonate

[@Pathologymadesimple]

Same proceudre

[@pikag9510]

Thanks mam