Next Generation Sequencing 4: Checking Nucleic Acids with an Agilent BioAnalyzer - Eric Chow (UCSF)

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  • Опубліковано 18 гру 2024

КОМЕНТАРІ • 38

  • @farrahphillips8461
    @farrahphillips8461 4 роки тому +7

    This video has been very helpful for me. Thank you for producing this well organized and thorough tutorial!

  • @tatyanazhebentyayeva1807
    @tatyanazhebentyayeva1807 5 років тому +9

    Very good presentation! Thank you! The only missing detail for beginners is proper storage of gel and other chemicals after opening kit.

    • @sm-kz4qt
      @sm-kz4qt 3 роки тому +1

      Pages 12-13
      www.agilent.com/cs/library/usermanuals/Public/G2938-90321_SensitivityDNA_KG_EN.pdf

  • @AqleemAbbas
    @AqleemAbbas 2 роки тому

    Dr. Eric Chou Explanations are very nice.

  • @aidajameei1552
    @aidajameei1552 4 роки тому +1

    Thank you s much. Hope you talk about RNA RIN value as well. Thanks

  • @bhangrafan4480
    @bhangrafan4480 Рік тому

    Excellent exposition.

  • @ylidene6222
    @ylidene6222 8 місяців тому

    Thank you! It helped me a lot

  • @potanii193
    @potanii193 2 роки тому

    When you said you are adding gel/dye mix to other 3 gel wells but you already added 1 out of 3 gel well the gel/dye mixture for priming. Do you have to add gel/dye mixture again on that well?

  • @orxanibayev340
    @orxanibayev340 2 роки тому

    Fantastic material, many thanks!

  • @Kill4Jill
    @Kill4Jill 4 роки тому +1

    Great Video Man! Keep up the good work !

  • @spindlesthesquid
    @spindlesthesquid Рік тому

    amazing video, so helpful! thank you!

  • @DaniloMicali
    @DaniloMicali 5 років тому +1

    That's a great and very helpful video! Congrats!

  • @vkuri2060
    @vkuri2060 4 роки тому

    Excellent video mate.

  • @Shaq0112
    @Shaq0112 2 роки тому

    Very well presented. thank you very much

  • @desireeheringlehner7842
    @desireeheringlehner7842 Рік тому

    I use the RNA Pico Kit but I´m wondering about your gel preparation. In my instructions it says to centrifuge the gel (spin filter) and add the dye AFTER (centrifuge again).

  • @Fattiapples
    @Fattiapples 3 роки тому

    Awesome video! Would love more.

  • @narminhamidli840
    @narminhamidli840 3 роки тому

    such a great video!!

  • @Genenat
    @Genenat 4 роки тому

    Fantastic video! Thank you so much.

  • @readme373
    @readme373 3 роки тому +1

    excellent

  • @konibei8786
    @konibei8786 5 років тому +1

    Love it, thanks!

  • @mostirreverent
    @mostirreverent 2 роки тому

    why is the latter separated into peaks, while the sample is a single peak?

  • @raquelrodriguesperes6948
    @raquelrodriguesperes6948 3 роки тому

    Great, thanks!

  • @Migshaw
    @Migshaw 3 роки тому

    AWESOME!

  • @deepakkumarverma4659
    @deepakkumarverma4659 5 років тому

    Excellent sir thanks

  • @lilianjose9154
    @lilianjose9154 3 роки тому

    Thank you very much for this video. It helps a lot! However, may I check on the reagents (especially all related to the dye) because as what I read in the Agilent High Sensitivity DNA Kit Guide, I understand that the reagents are light-sensitive. But in this video, it does not seem that way. Can anyone help in this matter? Thank you in advance.

    • @dominijerzboi
      @dominijerzboi 3 роки тому

      Hi Lilian, I use this instrument daily for my job in cancer research and in our lab we always store the reagents in a dark box due to it being light sensitive especially the dye that gets mixed with the gel as well as the other reagents.

  • @raposobernardo
    @raposobernardo 3 роки тому

    Hello everyone, how are you? I wondered how much it would be necessary to pay for a small lab capable of doing NGS? Can anyone help me? Thanks in advance! :)

  • @johnnykim233
    @johnnykim233 3 роки тому

    Very helpul thank you

  • @bluedrag00n
    @bluedrag00n 4 роки тому

    This rocks!

  • @anonviewerciv
    @anonviewerciv 4 роки тому

    3:20 13:20 Software.
    5:00 Hardware.

  • @sajanshrestha1613
    @sajanshrestha1613 4 роки тому

    I am a beginner, we are doing sequencing but is not show sequences like atcatcgatgc

    • @manuellessi98
      @manuellessi98 4 роки тому

      this is not sequencing, it is pre analysis of sample that will be sequenced

  • @sajinair870
    @sajinair870 2 роки тому

    Garu of love and Light...🤔🙃

  • @hskim352
    @hskim352 4 роки тому

    非常感谢

  • @sudhagc9796
    @sudhagc9796 5 років тому

    cool

  • @romeuribeirodeoliveirafsar7450
    @romeuribeirodeoliveirafsar7450 3 роки тому

    In