Sanger Sequencing Method (Chain Termination DNA sequencing) Explained

Sanger sequencing, also known as chain termination sequencing or dye termination sequencing is one of the most popular methods of DNA sequencing. It uses dideoxynucleotides, or ddNTPs, to create fragments of DNA of a certain length and a certain dye color, and uses gels to separate these fragments by length and identify the nucleotide sequence.
The process was developed by Frederick Sanger in 1977 and has been used extensively since. Today, it is used in smaller scale sequencing projects and to confirm the results of next gen DNA sequencing methods.

[@kmarie1102]

You explained very well and tied together the core principles of this experiment. Thank you!

[@Alkanator]

Good explanation! Helped a lot!

[@dkknkadkasndskdnksak]

This helped a lot!