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Приєднався 20 чер 2016
Next Gen SOLiD DNA Sequencing Method Explained
SOLiD sequencing is a next gen DNA sequencing method developed by Applied Biosystems. It's main advantage is that it is very cost effective and is better than other methods at detecting single nucleotide polymorphisms (SNPs), deletions, and insertions because of its use of 2 base encoding.
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Sanger Sequencing Method (Chain Termination DNA sequencing) Explained
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Sanger sequencing, also known as chain termination sequencing or dye termination sequencing is one of the most popular methods of DNA sequencing. It uses dideoxynucleotides, or ddNTPs, to create fragments of DNA of a certain length and a certain dye color, and uses gels to separate these fragments by length and identify the nucleotide sequence. The process was developed by Frederick Sanger in 1...
Thank you so much
PERFECT!
This is the best explanation of ABI SOLiD that I have found 🔥
ang pogi ng boses
Simple and effective...Thanks❤
really thanks
This is awesome,, i don't know how to thank you 🙏🏻
Thanks alot, very informative
this video was SO helpful and explained very clearly. Thank you
Thank you!
Excellent presentation Sir... Please do make more videos on molecular techniques in Genetics
Wonderful video thank you
Great work. Thanku so much sir Great presentation
Thank you for the wonderful explanation.
Excellent work!
👍👍
BRAVO!!! Thank you so much, this video is very clear and detailed. I understood everything!
very nice explanation! thank you
Wow this is amazing. I'm very grateful I've found this video. Thank you!
Thanks, Can you suggest some review papers
Thank boi
Thank you 🙏🏻
amazing explination . thank you
hi. may I request your references for this video? thank you
Very informative lecture thanks a lot🙏 😇 may god bless u with unlimited happiness and peace
luff yew
Well-put!
Hello, thank you for the great video. I am a non biologist trying to teach myself ngs analysis. I have 2 questions that might have obvious answers however I am not able to understand: 1) why to bother with "a base and color define the next base" when one can use 16 colors instead of 4 ? 2) what is the use of the 3 middle universal bases in the probes? i.e why not just ligate all di-bases at one cycle (instead of the offset 5 times...) and decode the colors ? thanks in advance!
You speak too much fast and the sound is so low
you saved me
I finally got it! thank you so much
What an amazing presentation!
great video still a little confused but definitely helped!
thanks so much for this video!
u should post a thank you video for 125 subscribers!!! keep up the good work!!
Congratulations, very informative video. Your simple and complete way to teach helped me a lot! Thank you very much!
* Permutation = each of several possible ways in which a set or number of things can be ordered or arranged.
thank you!
wonderful explanation... am struggling to understand this concept... now its very clear,Thank you
Nice explanation to every detail. Helps a lot, thanks bro !
This helped a lot!
It`s really nice video for understanding SOLiD. Thank you!
Thank you! I was having hard time understanding SOLiD-seq but this helped me so much.
hello Thanks for the excellent video. Could you please clarify something? The fragments of all unique DNA sequences are flanked by the P1 and P2 primers. Hence when they are binding to the beads, how is it ensured that each bead carries unique DNA sequences. These interactions are with the adaptor sequences right? Hence how is this possible since all DNA fragments carry same adaptor sequences?Thanks.
There are different adaptors for different fragments of DNA. The beads carrying DNA sequences(primers/ oligonucleotides/amplicons)are made by emulsion PCR and one bead is made in such a way that all the primers attached to it carry sequences complementary to any one type of adaptor, among many others. So, suppose there are 10 types of adaptors used to flank the fragmented DNA, there would be 10 different beads containing complementary sequence primers for those 10 adaptors respectively. Hope this helps.
Outstanding work. I'm sharing this with my entire molecular biology section.
Perfectly explained. Thanks a lot for this video.
You explained very well and tied together the core principles of this experiment. Thank you!
Could you explain in more detail how exactly polony formation takes place? Does the adapter-bound DNA curves as in bridge amplification or is it somehow unbound after the 1st amplification round only to serve as primer in an adjacent adapter?
this is a blessing. Thank you
Excellent! Thanks, finally I got closer to grasp this...