All makes perfect sense! Thanks for the explanation. Am wondering in terms of microscopy if the newer LED illumination offers effective Kohler illumination or just a purer white light compared to the older halogen incandescent bulbs?
The LEDs provide a cooler light (less red, less heat) and the color does also not change when you change the brightness (halogen became more red at lower intensity). The electronics can also ensure that there is no flickering and this makes video filming easier (shutter synchronization), but this depends on the individual microscope I guess.
This is why us old guys squint our eyes when we forget to grab our reading glasses. This give us a greater depth of field and we can see things up close, if there is enough light. Same thing with microscope condensers.
Thank you! A very informative and useful video indeed! I always wondered why the condenser had the possibility to be focused, since I always obtain the best results keeping in the top position. Now you explained it to me clearly. I also knew from experience that there is a "sweet spot" in the diaphragm aperture which allows the best balance between light intensity, resolution and contrast.
My scope has an incandescent light which seems to dry out the specimen, which obviously changes the view. Probably closing the iris keeps the specimen from drying out as fast.
Cx41 model. Im trying to adjust koehler illumination but condenser doesnt center whatever i do. I tried to adjust two secrews in almost every combination but it doesnt center. What else can i try to center the condenser light? Thank you for advance🙏
Der beste Kondensor ist ein identisches Objektiv mit Blende wobei beide optischen Achsen fest verbunden sind. Dies wird beim Heine- und beim pankratischen Kondensor teilweise verwirklicht
Hi am I saw your video about bacterial growth in a petri dishes, I have a question is it safer to grow mould in a petri dish and if yes how do you dispose it after wards
Is there any reason to lower a condenser - other than to get rid of dust? I.e., when do you need to set it to the lowest position? Or 1/2 way down? What happens to the image when you lower / defocus it?
Wieso muss denn der Kondensor zentriert werden. Seine optisch Achse sollte doch in jedem Fall mit der optischen Achse des Objektives uebereinstimmen und nicht mehr veraendert werden. Wohl aber sollte die Leuchtfeldblende zentrierbar sein. Das bedingt auch immer eine zentrierbare Punktlichtquelle.
I just got my first good microscope as an adult (had a few lower end ones growing up without the mechanical condenser). I have been spending a couple days learning to use it, I was starting to catch on and understand what you explained in this video, but you just sped things up for me and now I have a much clearer understanding of the condenser. So thank you for that. Good to know you just leave it in the highest position, I couldn't find any advantage to lowering it and I was a bit baffled by that particular adjustment.
@Microbehunter Microscopy, I hope one day you will have the time to redo some of your previous video by adding a visual support on what you are saying. For example, here, it would be nice to see what are the effect of the opening of condenser. I use to have it 99% closed and wanted to close it more (but it reach it's limit). I would also get a more powerful LED (3W instead of my 1W) to get the light intensity back. Unfortunately, you seem to have too many comment and you don't read them all. I can't blame you on that. Thank you for your tremendous effort to bring joy in this wonderful and inexpensive hobby!
Thank you so much for this detailed video on the condenser. I have researched how to adjust the condenser with the objective lens and have received information from my microscope company; however you have explained this issue so well. Basically you mention that whilst the condenser appropriate measurement is on the objective lens (x10 relating to .01 etc) you mention that scientifically that’s accurate however you use your eye viewing to adjust the condenser control lever to that which suits you…….. brilliant….I wasn’t aware of that option. Sorry I haven’t explained that very well. Brilliant video. Kind regards Ken from Rutland, England.
Thank you! Do the condenser and aperture have similar effects as phase contrast? Both utilizing refraction rates to get better contrast...? I am trying to choose a EUROMEX microscope at around 800-1,500 Euros for following Soil Food Web, and am confused with all the options I can choose from...
Thanks! I'm a biotechnologist, but at university they didn't teach us properly how to use an Abbe condenser, now I finally know! I guess I've not been using the full resolution potential of my simple home microscope objectives for years.. BTW When does lowering the condenser make sense? Is it only to "hide" dust on the lens out of focus? Because the slider allows it to be lowered a lot more than would be necessary just for that, but I've always kept it all the way up.
Really useful. I can’t do Koehler illumination on my microscope as no knob to turn on the illuminator but how to use the condenser on more basic microscopes is ignored in most discussions of condensers
It is very nice to listen to you, going back to the phase capacitor, do you have a recorded lecture on this subject anywhere? If not, could you ask for such a lecture?they say that other types of lenses are needed for proper observation
Does the condenser diaphram control affect darkfield beyond impacting just the light intensity or should you simply open the diaphram fully and ignore it?
Has MH made an episode on the whole DIC microscopy deal? All I know from "Journey to the micro cosmos" that is extremely complex and expensive, but it gives one hell of a sharp image. Perhaps a future crowd funding goal.
This is REALLY useful information. I wish I'd found it months ago.
All makes perfect sense! Thanks for the explanation. Am wondering in terms of microscopy if the newer LED illumination offers effective Kohler illumination or just a purer white light compared to the older halogen incandescent bulbs?
The LEDs provide a cooler light (less red, less heat) and the color does also not change when you change the brightness (halogen became more red at lower intensity). The electronics can also ensure that there is no flickering and this makes video filming easier (shutter synchronization), but this depends on the individual microscope I guess.
This is why us old guys squint our eyes when we forget to grab our reading glasses. This give us a greater depth of field and we can see things up close, if there is enough light. Same thing with microscope condensers.
Cool!
Thank you! A very informative and useful video indeed! I always wondered why the condenser had the possibility to be focused, since I always obtain the best results keeping in the top position. Now you explained it to me clearly. I also knew from experience that there is a "sweet spot" in the diaphragm aperture which allows the best balance between light intensity, resolution and contrast.
I knew there must be a use of that diaphragm. Thank you, this tip will improve my footage from now on. 😀
You are awesome @MicrobeHunter.. thank you so much for this video, it was very helpful!
Thanks! That’s very helpful!
Excellent explanation, thanks for the video.
Thank you sir
My scope has an incandescent light which seems to dry out the specimen, which obviously changes the view. Probably closing the iris keeps the specimen from drying out as fast.
Cx41 model. Im trying to adjust koehler illumination but condenser doesnt center whatever i do. I tried to adjust two secrews in almost every combination but it doesnt center. What else can i try to center the condenser light? Thank you for advance🙏
thank you, this is so informative. It's like finding the missing manual for microscopes
There are useful similarities with the iris of the human eye. Thanks so much. Im going to dig out my old medical microscope and see some bacteria.
Thank you very much for your kindness and for clarifying all these subjetcs (for free!). Great job!
It helped me to understand the Aparture Diaphragm ಥ‿ಥ
Der beste Kondensor ist ein identisches Objektiv mit Blende wobei beide optischen Achsen fest verbunden sind. Dies wird beim Heine- und beim pankratischen Kondensor teilweise verwirklicht
Hi am I saw your video about bacterial growth in a petri dishes, I have a question is it safer to grow mould in a petri dish and if yes how do you dispose it after wards
Is there any reason to lower a condenser - other than to get rid of dust? I.e., when do you need to set it to the lowest position? Or 1/2 way down? What happens to the image when you lower / defocus it?
Wieso muss denn der Kondensor zentriert werden. Seine optisch Achse sollte doch in jedem Fall
mit der optischen Achse des Objektives uebereinstimmen und nicht mehr veraendert werden. Wohl aber
sollte die Leuchtfeldblende zentrierbar sein.
Das bedingt auch immer eine zentrierbare Punktlichtquelle.
It did clarify a lot of things, thanks!
Informative 👍
Thanks! Yes helpful.
I just got my first good microscope as an adult (had a few lower end ones growing up without the mechanical condenser). I have been spending a couple days learning to use it, I was starting to catch on and understand what you explained in this video, but you just sped things up for me and now I have a much clearer understanding of the condenser. So thank you for that. Good to know you just leave it in the highest position, I couldn't find any advantage to lowering it and I was a bit baffled by that particular adjustment.
@Microbehunter Microscopy, I hope one day you will have the time to redo some of your previous video by adding a visual support on what you are saying.
For example, here, it would be nice to see what are the effect of the opening of condenser.
I use to have it 99% closed and wanted to close it more (but it reach it's limit). I would also get a more powerful LED (3W instead of my 1W) to get the light intensity back.
Unfortunately, you seem to have too many comment and you don't read them all. I can't blame you on that.
Thank you for your tremendous effort to bring joy in this wonderful and inexpensive hobby!
Thank you for this helpful video. Nicely described. ♥️
Thank you so much for this detailed video on the condenser. I have researched how to adjust the condenser with the objective lens and have received information from my microscope company; however you have explained this issue so well. Basically you mention that whilst the condenser appropriate measurement is on the objective lens (x10 relating to .01 etc) you mention that scientifically that’s accurate however you use your eye viewing to adjust the condenser control lever to that which suits you…….. brilliant….I wasn’t aware of that option. Sorry I haven’t explained that very well. Brilliant video. Kind regards Ken from Rutland, England.
Excellent video but probably needs some demos to highlight the various points made.
Thank you!
Do the condenser and aperture have similar effects as phase contrast? Both utilizing refraction rates to get better contrast...?
I am trying to choose a EUROMEX microscope at around 800-1,500 Euros for following Soil Food Web, and am confused with all the options I can choose from...
This is a very helpful video. Thank you so much!
Thanks for the explanation! Very helpful.
Amazing! Can't wait to explore more of your videos.
Thanks! I'm a biotechnologist, but at university they didn't teach us properly how to use an Abbe condenser, now I finally know! I guess I've not been using the full resolution potential of my simple home microscope objectives for years..
BTW When does lowering the condenser make sense? Is it only to "hide" dust on the lens out of focus?
Because the slider allows it to be lowered a lot more than would be necessary just for that, but I've always kept it all the way up.
Really useful. I can’t do Koehler illumination on my microscope as no knob to turn on the illuminator but how to use the condenser on more basic microscopes is ignored in most discussions of condensers
Thank you very much you're better than my professor lol
Thanks for your clear explanations!
This was a super helpful video, thanks!
Is this necessary to have on a microscope for doing fecal parasite testing?
It is very nice to listen to you, going back to the phase capacitor, do you have a recorded lecture on this subject anywhere? If not, could you ask for such a lecture?they say that other types of lenses are needed for proper observation
Fantastica spiegazione, mi ha fatto capire tanti ssime cose. I have to thank you so much
Excellent explanation! Thank you!
Very helpful Thanks.
Really clear detailed information excellent video
excellent stuff, perfect explanation
Thank u! Great explanation
Really useful thank you!
Fantastic explanation
Thank you so much~🥰
You are a great man.
Does the condenser diaphram control affect darkfield beyond impacting just the light intensity or should you simply open the diaphram fully and ignore it?
You need to open it completely, otherwise it blocks all of the light.
Has MH made an episode on the whole DIC microscopy deal?
All I know from "Journey to the micro cosmos" that is extremely complex and expensive, but it gives one hell of a sharp image.
Perhaps a future crowd funding goal.
I also have now DIC. Check my other channel for videos. It's great.
@@MicrobehunterMicroscopy Nice!
Thank
excellent!