Why a 3000x microscope magnification does not make sense!
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- Опубліковано 15 сер 2018
- Here's some microscope buying advice. Amateur science and amateur microscopy is becoming increasingly popular. Some companies are advertising their microscopes with unrealistically high magnifications. Some comments mention that the reasons were not properly explained in this video, so I will add this information here. [video: 048]
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This same scam applies to telescopes. In reality, the maximum usable magnification of a telescope is just a little bit more than the diameter of the objective lens or mirror in millimeters. A 200 mm primary mirror maxes out at about 240X. Yet manufacturers will claim magnification of 1000X or more for scopes with 50 or 60mm objectives.
Yes, and the purpose of telescopes is also to capture light, to make faint stars (etc) visible, and not only to magnify. I used to be (still am a bit) into amateur astronomy as well and when I bought a telescope years ago, this issue about unrealistic magnification was already covered in magazines. I guess consumers want to have a simple way of comparing the quality, and magnification seems to be the most easily understood one, even though it's not the best measure.
I spend a lot of time photographing stars and you are right. There is a huge difference between magnification and what can actually be resolved. Additionally, those high-end magnifications you need very solid (read heavy) tripods and the ability to track stars as they move.
On top of that the higher magnification you go, the smaller the exit pupil. So not only is the image getting blurrier as you go higher, but it's getting dimmer as well! That's why larger aperture telescopes show so much more detail. You're can hit higher magnifications with a larger exit pupil.
Exit pupil is the size of the image that gets reflected from the telescope. Basically what gets projected onto your eye through the eyepiece. If you have a pupil that's 7mm fully dilated, and an exit pupil that's only 0.5mm, you're wasting 6.5mm of light gathering from your eye. (At least that's what I remember it being back when I was super into this stuff, if I'm wrong someone correct me.)
@@JesusistheWaytheTruth288 Dilated pupils......So that's why people do astronomy in the dark. :)
@@AverageJoe8686 Thanks. I do actually spend a lot of time photographing stars. And you are right, slightly off-center is helpful though it feels strange at first. To me, the Andromeda Galaxy is just a faint blur when not looking directly. If I look straight at it, then I see nothing.
This is great. Everyone should watch this video before purchasing their first microscope.
That is exactly what I'm doing right now Haha
Olav Viking no, it’s a scam. All they’re doing is changing the eye piece lenses to achieve higher magnification without taking the rest into account. Hence, why it looks like shit at 3000x
@@kevinarzola4781 You've done no industrial microbiology. Do some, get back to us.
@Bleep Bloop Industry uses enlargement all the time. You know, the companies that pay for these things?
BeeRich33 lmao what I said is literally proven. It’s scientific fact. The man even holds your hand to explain it
You saved me from doing a stupid purchase. You explained my worries and questions so well. Thanks a lot for letting us know this valuable info!
I actually love your intro... It feels nostalgic. Like I'm back in grade school getting ready to watch something the substitute found in the library
the zoom in got me lmao
"Snap!"
I was wondering what you were talking about
Then it happened.
Scanning electron microscope:
"Hold my beer."
Helium ion microscope:
"Hold my cognac."
@@FinnMcRiangabra iPhone macro lens:
"hold my soy milk shake"
Eyes:
"hold my eyelids"
STM: "Hold my electron.. ah shit they tunneled through may hands"
@Crimsonfireball, Eyes: "hold my dirty glasses"
I know from watching CSI, you just have to say "Enhance" it you see more.
The guy is correct, and accurate.
But he NEVER ACTUALLY GETS AROUND to explaining why.
I.E. good scientist, shyte teacher.
The explanation *why* is mathematical. I will do a video on that.
@@Microbehunter Looking forward to it! Subbed.
Visible light or optical microscopes can't resolve details smaller than 1 wavelength. From violet to red, that wavelength ranges from 200nm to 700nm. Since blue light has shorter wavelengths, it would be possible to see clearer details in filtered blue light than red. Why is 1 wavelength the limit? The reason is that light is a wave so it diffracts or bends at the scale of 1 wavelength. Why do the images made by interfering electromagnetic waves get blurred by 1 wavelength? This reasoning is getting deep enough that it is hard to explain.
Anyway, you can still resolve finer detail with a microscope. Resolving finer details just requires a jump to a completely different type of microscope such as an electron microscope or x-ray microscope. If websites selling electron microscopes listed their maximum powers, that would be really funny since I doubt the typical electron microscope shopper would be swayed by those numbers.
@@iarrcsim2323 All perfectly true, and all *irrelevant* to the practical limit of visual microscopy limitations.
The limits imposed by wavelength limits are *several* magnitudes smaller than the limits imposed by practical objective diameters.
How is it correct or accurate then? Using digital zoom as an analogy doesn't really make sense either unless you're talking at the level the commentor above mentioned where the wavelength is the smallest scale.
Taking a digital photograph and zooming in looks like shit because there is no more information there. You're looking at a representation of a sample of light taken by a sensor; usually a square that is not accurate measurement of the actual light as it would be observed, but an 'average' if you will that is assigned to the closest match available.
From what you and the video poster are saying it is something different.
Well yeah, it's just like optical vs digital zoom. The microscope companies should advertise in terms of resolution, otherwise it's just misleading.
It's not quite as simple as that. Using visible light it's not possible to resolve anything meaningful below a certain size as even blue light has a wavelength of about 0.0000004 meters (0.0004mm). All you would get magnifying something that small up is a blob regardless of lens quality.
@ateb3 I'm under the impression that it is down to lens quality and alignment. I do not know for sure though.
Randomly at exactly 4:37am I have found out I want this to be another hobby of mine and realized I should look up things to have a better understanding. I’m glad I did bc all I was focused on was the magnification. I’m glad I found this video. Thank you. You saved me a lot of money from people likely praying on beginners like myself.
Translation, you won't get good focus at high magnification.
It's not about focus, it is as he says about resolution.
Ok
I was missing the explanation too. The explanation you added to the text is great! I think it is not possible to explain it that good in the video anyway, so now it's way better than it could have been originally.
I agree the most part but i don`t think more than 100x is a waste. I have a 1976 microscope with 4 10 40 and 100oil and oculars ranging from 5 to 15. And to do the jump from the 10 to the 40x objective i put the 15x ocular with the 10x objective first so as an in between magnificaiton and it doesn`t loose any detail. What i mean is that the 10x objective for example has enought detail to magnify it`s image like 25 times with the ocular without loosing resolution (at least not perceptible)
Okay, I get it. Then what specs should one be looking at? You said 'use other criteria' but it will help to actually spell them out.
Thanks for clarifying this! This always confused me how they got so high magnification.
Just found your channel! Really cool. When I was smaller I used to want to be a microbiologist but now that I’m 13 I want to be a pilot, but still love microbiology
Thank you very much for your video, it is really very helpful for someone who is starting in the hobby as me. But I didn’t understood how can I determine the useful amplification for a set of lens. How can I choose between different lenses? Is there some kind of specification that determines their quality, or “sharpness” in certain amplifications?
Yes, there is a number (the numerical aperture or NA) printed on the lens, and the higher the number, the better the theoretical resolution. Higher magnifying objectives have a higher NA. BUT: this is only theoretical. There are many other factors that determine lens quality and there is no way that you can see this from the outside. However, for all practical purposes this does not matter in most cases and often you are not given a choice anyway when buying a microscope. Apochromatic objectives are better, but almost not affordable and you only get the image quality if your specimen is prepared properly etc. Watch the video "Microscope comparison - From very expensive to super cheap" to see a side by side image comparison.
Microbehunter thank you very much! I will check it out!
Same
don't know how I end up here, but I'm not complaining.
Great review and information. Thanks and I have subscribed
Wow, I actually learned something today. Great explanation. If it wasn't for your visual examples I don't think it would have made much sense to me. 👍
When I was in 7th grade, my science class had some group projects and we got points off on ours for not listing a 1500x or whatever under a picture we had on our poster board. Thing is, it was blown up from an image in a book and the teacher wanted the book's magnification level on our blown up image. If you think about it, a magnification factor really only applies to the virtual image at the ocular lens. Your eye or camera will be receiving an image that's about half an inch or a centimeter wide. Take a picture and put it in a book or on a poster board and that factor no longer applies. The photography and printing affect the scaling of the image and therefore "magnification factor". Blew my mind that as a 12 or 13 year old kid I had to explain it and was still told I was wrong.
You can make a microscope image larger by printing or displaying it on a big screen. But that does not increase the resolution - the amount of detail you can see.
tx for the explenation. So, what is the difference between low end and high end microscope if everything share the same features? is it only the quality of the device?
In short: A. mechanics (manufacturing quality). B. modularity (exchangability of parts) C. accessories (brand microscopes have many accessories because they also manufacture for medicine, research etc.) D. reliability. But I would say that most mid-range microscopes are really fine, especially for amateur and educational use. Some high end devices allow for additional features that are not necessary for amateur and educational use (fluorescent microscopy, DIC, phase contrast, specialized polarization microscopes etc). I made another video, where I compare low and high end microscopes.
I learned a lot.
Thanks for the video, man.
Great video. I hadn't even thought about this, although the last microscope I used was an electron microscope so my expectation of what an optical microscope would be able to achieve had been slightly distorted! It's the same as this with telescopes also, even things like planets look much better at lower magnification.
Hahahahahahaahah when he zoomed into his mouth 😂😂😂😂
Thanks for the infos, now if i were to look for a microscope what would be the specification I would need to recognize in order to have a good structure resolution of what Im magnifying ?
Guess you'll never know :c
Great video. Your point was made very clear. Love your channel. Helpful imformation, thank you.
Thank you for this information! I am so happy when I come across a person who is more knowledgeable than myself on a topic, great!
Great stuff. Thanks!
What stereo microscope would you recommend up to $300?
I can not recommend anything particular, because the choice is so large and so much depends on individual needs and preferences. There is a saying: "The best microscope is the one that is used most often."
No, you obviously need magnification and resolving power as well. But, yes, beginners are attracted by magnification. A single piece of perfect glass with no magnification so no lensing has maximum resolving power DEPENDING ON THE SOURCE OF WAVES, light or electrons so all details are preserved but you can't really see much of the details without using lenses
Once you introduce lenses the image is distorted. The better lenses you have the better the resolving power
For high magnification the resolving power becomes more important. Apochromatic lenses have less chromatic and spherical aberration and thus better resolving power. But even in low magnification they offer a sharper image. For example if you have a camera and a good objective lens you can digitally zoom and get more details than with lower quality lenses.
Better quality lens are more expensive not higher magnification as explained in this video.
Having the best possible lens is still not enough for magnifications over 1000x because the resolving power is limited by the wavelength of light. The higher the frequency / lower the wavelength the better resolving power. That is why electron scan microscopes are by far superior because electrons have much lower deBroglie wavelength.
If only the video had included any of this information...
U mean...not enough light to see through 3000x?
Awesome video. I am late to the microscope-party, (just getting started), but this video helps me out big time. It is on point, it has loads of valuable info and is very good to follow for everyone. Love it! Thanks for sharing. Gonna dive right into your other videos. 🍻
Man, this vid made me sad.. but really appreciate the heads up and the free guidance!!
Really liked this. Almost everything you mention also applies to astronomy as well. Insane magnification leads to no or horrible imaging.
4:49 is when the video starts
All his videos need a time stamp when it starts, he talks soooo much
As in "Here's where there's one picture of what he's talking about, who cares the reason I just ant pretty pictures"?
Yeah, very informative.
Now that is a jumpscare
What kind of adapter that u use for adapting eos camera to trinocular? I just bought a new microscope Olympus BX53, and i dont have an option to adapt my eos dslr canon camera to my microscope
Hello, Connecting a DSLR to the BX53 works well and gives you good results, because there are no intermediate optics. The camera must have an APS-C sensor (small seonsor amd not full format) otherwise you will not get the full field of view. You need 3 items: U-TV1X-2 and U-TMAD and a camera-specific T2 adapter ring. You can get these adapters directly from Olympus or second hand. (There might be a plastic foil in the adapter that you have to remove to get the full field of view).
@@Microbehunter i just have U-TV0-5XC for be connected to C-mount camera. And i also have T-Connector to be used to my small microscope (107Bn) I think for having a high frame rate i decide to use my canon eos camera to my olympus bx53. But i dont have option. I try to find store online in my country (indonesia) but i cant find it (T-MAD)
@@PenyuluhPertanianLapangan Try to buy it directly from Olympus if you can not find it second hand, even if you have to import it. It will not be cheap, but you will get a really good image quality and a large field of view.
@@Microbehunter okay, thank you for your friendly response.
Extremely informative and well done. Thank you very much.
You took the first 4 minutes to convey a short sentence worth of information. I had to check the timeline because I thought I restarted the video by accident somehow.
In the first minute you show some Müller brand microscopes.
Please note that "Müller Optronic" or "Müller Germany" is not a German optics manufacturer.
"Made in Germany" is a protected attribute, "Germany" is not.
Müller is one of the most common surnames in Germany. In the recent years a couple of Chinese companies have started brands with German names.
Yes,a couple of years ago, someone in China manufactured sport shoes branded as Uncle Armor
@@aliszhinchaenz That reminds me of a set of "Pieonear" headphones.
@@MetalheadAndNerd lol
Not as good as Acliclas.
@Jakob Jones China is fully capable of making high quality, high precision goods. They seem to be obsessed with flooding the market with 2nd and 3rd rate (or worse) junk because people are obsessed with buying it. If the average consumer was more demanding, a lot of this junk would disappear.
you have any links with more magnification and other modifications its kinda serious you know something where I can buy a microscope that cam let me look closer? then 400x magnification??? what is a better measurement to use & a real authentic microscope?
@Microbehunter
In our paleobotany department we've got an old microscope that had a camera once but the camera does not work anymore. My Prof told me that it got excellent hardware/optics and its a shame the camera is broke and there is no simple replacement available anymore.
So here is my question: What is the "tube" called you use to mount the DSLR to your microscope, and how can i figure out the requirements for the "tube" to be able to mount a DSLR to the microscope. He would sure be happy if there is a way we could get it running again.
Thanks in advance
.
Hi Microbehunter,
I have just discovered your channel through this video, and I have to say I am very impressed, this exactly what I have been waiting for.
This channel should be a first port of call for any student or amateur (like myself), I have just subscribed and I cant wait to see your next video.
Thank you,
Anthony Mataabro.
.
I have 100x objective
and 16x eyepiece which makes 1600x magnification
of my microscope, was this really true?
Correct. But it will be blurry, therefore there will not be many details visible.
Yes...it's true. If you like what you see, then you are ok.
It is true. However, it is going to provide no further _resolution_ over one with 600x magnification. That is a function of the illuminating wavelength, and for visible light that pretty much caps it at around 300ish nm or so. No feature smaller than that will you have any chance of seeing. The only way to change this is to change what you're illuminating with, and that requires a completely different and often dramatically more expensive microscope, e.g. electron microscopes.
Thank u. It was so helpful.
Thank you so much for this video! What would be the other criteria that you would look for in a microscope for beginners?
You want 3000x with good resolution, use a STEM. Visible light just hasn't got a small enough wavelength to resove fine details. I wonder though why they don't use EM waves of a shorter wavelength like UV with a photodetector that can actually "see" these wavelengths. Though something tells me they do.
I looked into it and yeah, UV microscopy is a thing. As is microscopy utilizing soft x-rays in the same manner. But apparently you can tune electrons to almost any wavelength. So that's why STEMs are more common.
Maybe you can use a UV light source and then use a UV camera to take photos. Might save you a few million dollars.
Let me just pull out a quarter million dollars and grab one
@puckay Put sunscreen on them.
Electronics uses both X-Ray and Electron microscopes. When you are trying to resolve things with a size heading for 1/10,000 the wavelength of visible light it starts getting a mite hairy. EG fault "blips" on the line widths of .07nm lithography.
Thank you! As a Med-Bio student it is releaving seeing and understanding the specificity and the point of your advices. I am both glad and gratefull to you, for this clip. The only true microscope propper buying advice. Thanks to you, today i gained a few more criteria when considering purchasing my new ''toy''. :)
Thank you for explanation, but if not magnification, what specs should I pay attention to?
As an amateur (actually not even that) thinking of getting some sort of magnifying device to examine the edges of knives, I found this video very enlightening.
Thankfully to date I have only wasted a few bucks on cheapo digitals off Ebay.
Thanks for posting.
This guy talks fast. I had to turn it down from the usual 2x speed I watch all videos on to 1.5x speed.
You really didn't have to keep repeating the difference between magnification and resolution endlessly. I got it the first time.
Hey.. Great video..what is the name of the canon camera adapter and where can i buy it ?
Excellent explanation. Very useful and informative. Thank you and congratulations on the channel!
But why haven't you listed these OTHER criteria for buying? What is the really important parameter to look for if we should ignore the magnification figures? How do you state a resolution of the microscope, for example? Is the focal length an important parameter?
Looking at examples of specimens through the 'scope is the best way to evaluate. NA (numeric aperture) is nice to know, etc. But you might have a 'scope that looks good on paper, but has chromatic aberrations, or weird-but-subtle internal reflections, or focus shift with change in magnification,...
The poster doesn't seem to have a firm grasp about the subject. The resolution of all light microscopes is exactly the same, however lens defects lower the effective resolution, therefore the best microscope is the one with the best made lenses, unfortunately there is no real metric for this. The best you could hope for is for a company to state the size of the smallest object that microscope/lenses can resolve.
Stop beating around the bush and explain why. I'm halfway through the video and it's beginning to sound like a shaggy-dog joke story.
watched the whole video and i still dont know why, the only example given was blurry due to resolution which does not apply when doing optical magnification...i have no doubt that magnification numbers over certain point start to be pointless and higher end microscope with less magnification will be better than cheap chinese microscope with high magnification, but why....beats me and i dont think this guy understand why either, hes the user, he knows its there but he cant explain it
@@ZomgPL I wish he'd do the whole thing again but to the point. All I could gather was that he was really just stating the obvious and that is a clear distinct photo/video was better than a blurry one and the best way to do that was to concentrate on getting good resolution and depth of field and not so much on the power of the microscope.
@@MrStringybark i suspect it has something to do with focus and how good of a quality the lenses are, as in without defects, i dont really get why he even talks about resolution in optical zoom...
@ZomgPL
There is something called diffraction limit. Basically you can't distinct details smaller than the wavelength of the light you are using.
Visible light has a wavelength of about 400-700 nanometer, or 0.0004-0.0007 mm. This is the very smallest objects visible with normal light and optics, meaning that some object of that size simply cannot appear as more than just a very blurry dot. Around 0.5 mm (at 1000X) is ample size for viewing a blurry dot, it does not at all become more clear by being a 1.5 mm (at 3000X) blurry dot without any more detail.
If a company advertises anything more than 800x - don't buy. They are marketing kids 'scopes to innocent adults.
Good Video. I have a question: I have an issue with my microscope that I bought a month ago.. I cannot see anything with my 40x objective lens unless it is touching the slide (which should not be the case). What can be done? Because of this I haven't yet used my 100x oil lens.
Do you have a diopter adjustment (rotating eyepiece)? It could be off. Make sure that the objective is screwed in completely. If you use ready-made permanent slides, make sure that the flat surface of the slide is on the bottom (specimen on top).
10 minutes of rambling, and you can't bother to explain WHY? This video is about 8 minutes too long for what was actually said.
magnification is limited by the wavelength of light - you can’t see things that are only a tiny fraction of a wavelength in size. The most you can ever hope to get out of a light microscope is about 1500x, which will let you see objects as small as 200 nanometers in size.
Also the blurry pic is 4x and none blurry is 40x so why did he say they were the same?
@@punkisinthedetails1470 he zoomed in on the 4x pic to get the same 'magnification' but he doesnt seem to explain why the 40x is more clear other than the normal viewer knowing about optical vs digital zoom
@Bleep Bloop well thats the only difference that is shown in the video about why maginification doesnt matter
The maximum useful magnification that can be achieved by an optical microscope typically ranges from 500x to 1500x. Higher magnification is possible, but increasing magnification past a certain point results a decrease in resolution.
While both the ocular and objective lenses are responsible for the final magnification on a compound microscope only the objective lens is responsible for resolution.
You need to take into account the *numerical* *aperture* of the objective lens.
Numerical aperture can be defined as being equal to the refractive index of substance between the specimen and the objective multiplied by sin Mu
Mu being 1/2 the *aperture* *angle*.
(sorry Mu should be the lower case Greek letter, UA-cam comments do not seem to allow Greek letters)
The aperture angle is the angle described by the cone of light that enters the objective lens after passing through the specimen. This will depend on the curvature of the lens and also on how close the objective lens is to the specimen when it is in focus.
There is more about microscope magnification and resolution on the Nikon site.
www.microscopyu.com/microscopy-basics/useful-magnification-range
Interesting and useful, but there's no need for this video to be almost 10 minutes. There's too much repetition, I would cut it down to 5 minutes or less.
John Grey longer videos = monetization optimization
Tarik Essamri yeah but if you know anything, you’d know the 10-min mark gives a big difference in revenue on this website, which he didn’t hit, which invalidates your stupid talk
dillon I hope u have a cancer :)
dillon I know a lot Dillon...and I know u'll get cancer :)
Thanks, I really thought, that the magnification ist very important. You opened my eyes. What criteria are important if you want to record to DSLR ?
Hello, I have a question,
I've recently started using a microscope camera more frequently instead of looking through the eyepiece, as I find it more comfortable and also to take and share pictures more easily.
When using the eyepiece, the final magnification is calculated as such: the objective magnification (x4 to x100) times the eyepiece magnification (I have x10 and x25). I've seen in some of your videos that you also could add x2 intermediate piece for even bigger magnification (even if pointless).
Now, when I use the camera, there isn't a final eyepiece that "scales things up even more". So the maximum magnification I'm able to achieve, optically, is x100 (but I don't like using oil so I keep it at x60). I'd like to know how to achieve, on camera, more than x100 magnification. I've seen videos of Microcosmos where on camera he has x400 at times.
I know it will not define the picture better, but it'll still be better to optically upscale images to see the details than to digitally upscale it I guess!
So, what tool do I use to go from x60 to x400? Do I need to put multiple "intermediate pieces" that do like x2, going to x120 then x240? Or is there another solution? Thanks!
ua-cam.com/video/7xhBrbMu-YU/v-deo.html
Very convoluted explanation. And too many repetitions. You can do better.
@White Rice pls tell me why some microscopes with high magnification are no better than ones with lower magnification
From a certain magnification onwards (about 1000x), you will simply see everything more blurry but not more details. The spot that you see will only appear larger but small details will not appear. You will not gain more information from a certain magnification onwards.
@@Microbehunter Yeah dude you said that like 12x in the video but never explained why. Honestly this video could have been about 30 sec long and conveyed the same amount of information.
@@Microbehunter That is simply not true. Light microscopy can resolve objects 0.2 micrometers across, a magnification of only 1000x would make that appear only 0.2mm across. That is not enough to make them easily visible, even though they can be resolved. 10,000x magnification is thus completely justifiable on a sufficiently high quality light microscope.
I like how maybe a month ago I accidentally happened to watch that one random microscopy video of some bacteria or whatever. Since then UA-cam has recommended me more and more such videos. Now I'm watching this and thinking maybe I should get a microscope.
I want a microscope I can use when looking at the spores of fungi. Will
Bresser 50x-2000x LCD Mikroskop
work for that purpose?
thank you, you are still helping out people that's only ever used the cheap 200 and 300 power scopes i need to see blood cells i like you said was just hunting down the highest i could find money aside you saved me a learning curve so to speak. subscribed and will check out your view on things.
1600x with really good lenses and immersion oil makes absolute sense over 1000x.
We,ve got some pretty highend Leica scopes at my job and some of them go up to 400x and i can tell you , you can't see sh7t at that magnification!
@@jclouds2257 I'm not saying they're crap. At 300x they're perfectly sharp. Just around 350x and above they get very blurry.
Also they are stereoscopic and you can't really perceive depth at anything above 200/250x
We use them to inspect microchips so they don't really need to go that high but still.
I want to buy a microscope, which one do you recommend? im new.
Your advices surely helping microbiology students like me
THANK you
comparing optical zoom with digital zoom doesnt debunk anything. 4:50
you don't talk about why those higher powers don't work. your example doesn't work either. by zooming after the fact you are reducing the resolution because there are fewer sensors being hit by the same area of sample. with your eye it's going to still be projected across the whole surface. I remember doing oil immersion in high school, so i'm guessing we're dealing with a refractive index issue at this level of magnification or are we also coming close to the resolution limit of light at those powers?
Thank you for these informations!
Thanks for good pointers!
3000x magnification vs use camera to "zoom and enhance". I think we've traded one fallacy for another.
A whole lot of talking with very little said.
Very educational. Would you say the same thing with cameras? Like the Nikon p900, p1000.
The magnification on his mouth got me crying from laughter. Solid comedy gold.
why am i watching this?
cause of the music
- A 1 mm² size object or area (1 mm x 1 mm) times 1000x will be like 1000 mm² or around 3.1cm x 3.1cm in area size. For example a tardigrade.
- The biggest virus is Megavirus with 440nm diameter, so the area is 607904 nm² or 6.07904e-7 mm² magnified by 1000 then 6.07904e-4 = 0,000607904 mm². It's still too small for a microscope to see a virus then.
If even 3000x magnification doesn't make sense, then how do people see a virus ?
I just googled it and turns out this kind of microscopy is called light microscopy (max around 1500x) while for viewing virus will need electron microscopy (can be 1 or 2 or 10 million times). Funny thing is, tardigrade looks transparent under light microscope but looks solid under an electron microscope which can only see through skin in nm thickness while tardigrade is in the mm size, so that's why there are various images of tardigrade lol, transparent and solid view.
I think viruses seen under electronic microscope, and here they are talking about compound microscope
@@T1k3mys0n The tardigrade would have been gold plated for viewing in the scanning electron microscope.
@@T1k3mys0n You don't really "see" things under an electron microscope. Usually they use the word "visualize." Much below the wavelength of visible light, objects have no appearance. An electron microscope detects a pattern, which is then converted into a visual image for our sensory convenience.
You would need a fine adjustment on your fine adjustment just to center the sample.
Is there any way to compare the resolution? Do they mention resolution in the product? Can it have a number?
That side-by-side comparison makes the topic seem clear. Pun intended. Excellent explanation and demonstration!
Insisting that a microscope's purpose is not to magnify is one of the stupidest things I've heard in my life. I can see he's trying to make certain points about the qualities of microscopes but he is going about it in the worst possible way.
CLICKBAIT! No explanation is given just the same thing repeated over and over again.
Thank you so so so much! You have taught me a lot.
I'm interested in mycology. I've been looking at a microscope with a 100x objective that can be used without oil immersion. Is this good or will the image be too bad to be of much use?
The digital zoom analogy is appallingly bad. Microscopes use optical magnification which is entirely different.
Not if you know the physics. IE for blue light the "resolution" has a theoretical limit of 0.0004mm for red it's worse. So magnifying anything up that is smaller than that looks very like and acts very like digital zoom.
@@gordonlawrence4749 This 10 minute rant doesn't explain that at all. That's why it's an appallingly bad analogy.
@@gordonlawrence4749 whilst that is true, 1000x magnification still leaves those objects at around 1mm in size. Still small enough that further magnification is easily justifiable.
@@joestevenson5568 Please read and understand a post before replying to it.
@@gordonlawrence4749 Can't you "super sample" it if its at low resolution - like displace the image by fractions of your maximum resolution and reconstruct a higher resolution image ?
This video could’ve been 1000x shorter.
But not necessary higher quality.
Useful info sir thanks
Thanks! It's very helpful
your confusing magnification with zoom.. for 10 minutes
No. You're confusing magnification with zoom. They advertise high magnification, because that's exactly what they do.
Thanks for wasting 9 minutes of my time.
Magnification is the same thing as zoom. A zoom lens in photography magnifies the image when you turn the focal length from the smaller number to the larger number.
You aren't very bright, are you
Any ideas which is the best electron microscope suitable for fixing my Sony A7s ii on it ??
Which gear required for that ??
Thanks a lot. This is very useful.
I think you did a great job pointing the issues out with the magnification BS ...
Great video bud but I have one question for you. What are those batteries in the background that look like LiPO batteries with Dean's connectors on them?
They are LIPOs with Dean's connectors! I forgot to put them away. I am also into making model airplanes and experimented with a small quadcopter...
, thank you for clearing this up for me I had no idea
What is the best microscope that I can buy to see bacteria and blood cells at the biggest and for home use
My question was a bit vague. I should clarify. I mainly want a microscope with depth of field for examination of workpieces, often handheld, so it needs to have a 20 or 40x sort of option. But I also would love for it to go super magnified for looking at tiny details. Are AmScopes any good? They have ones with x20 X40 X80 for $300 or so.
You need a stereo microscope. If you want to see tiny details (not of your workpieces) as well, like all the way up to 400x, then you need a compound microscope. You need to get two different microscopes.
Cool thanks! Is there a way to mount a single x400 setup on one of the eyepieces of a stereo microscope? Or is it a completely different system?
It's completely different and you can not mount this. There are physical and optical limitations. And also the objects that you can see with them is different. It is not uncommon for people to own both types of microscopes so that they can look at more specimens. This video explains it: ua-cam.com/video/-yxD-JwUJ6o/v-deo.html
Thanks! So I wonder, when you photograph through the third "hole" or what to say I realize that you will not get more then the objectives "magnification" ? correct?
I have a Swift 380-T that I connected a Sony a5100 camera to on the top but I only use a cheap metall adapter that has no lens in it, only cost lik 15 USD, will it be better or what will be the difference if I buy the kind of adapter that has like a built in lens to it and cost more then my microscope?
Glad IF someone could tell me this 😇😀
Love this channel! Thank you for doind all these great videos for us (yes this one was an old one just showing up that I actually did not see before, And I have seen a lot 😄👍)
Thanks so much for the information,I am going to buy a microscope and now I know better. Thanks from Sweden!