🔬 Upgrading a microscope to darkfield | Amateur Microscopy
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- Опубліковано 30 вер 2024
- It's quite easy to upgrade a microscope with a condenser to darkfield.
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Your recommendation of the Swift SW380T was good timing. I upgraded to this microscope and find it excellent for my photography goals. I will try the dark field, thanks for that too. For those of your viewers who have this model, I have a measurement that will be critical when using the trinocular camera mount with their DSLR or mirrorless camera. When I called customer Support in Texas, they could not tell me the focus distance the camera’s sensor needs to be.
If you measure the distance from the base of the black camera mount (where it meets the blue-ish base, to your camera’s sensor, it should be close to 90mm +/- 10mm. What this means is that if you are using a deep t-mount adaptor, your camera may be too high. I am using a M4/3 Olympus and I had to order a thinner T-mount adaptor. Once you get you camera lower down, the imaging should be great.
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Oh my GOD. That is incredible! I need to tweak it, I think I’ve got the black part off centre, but it is absolutely astounding. Thank you so much for this! I have the SW380B, and I can’t believe an instrument I own is capable of this!
I have been using dark field for a while but I sill learned from this video. Outstanding.
If you are looking at something non-transparent, such as a computer chip, does this method of imitating DIC still work?
I made and used oblique illumination to look at a jelly fish I found on the beach it looked AMAZING!! I wish I could share pics/video in the comments I only have the 380B swift but it looks professional! Deff take this guys advice he has never let me down!
Dark field condenser is the way to go.
I used the clear plastic and paper method, I am perplexed. 4x and 10x objectives I get dark field, if I use 40x or 60x it looks like bright field with the light turned down too dim. I have tried sizes ranging from 15mm all the way to 24mm. I can get oblique illumination to work beautifully, which looks amazing I have to say.
Awesome explanation, however my condenser does not have a filter holder ( abbe 1970) is it possible to have the same effect if you place the stop patch on top of the iris?
It should be possible, but will definitely require a different size. What you are doing is getting rid of the light pathway that directly hits the microscope objective but leaving the light pathway that would go around. So no light enters the objective directly from the light source, only if it is scattered by something - i.e. your sample
Thank you for the video! Could you share the stl files for these darkfield filters?
I have the exact same microscope as you from video #6 (the educational one in the middle). It does have the ability to add darkfield, and I 3d printed many shapes to try out, and it works well at low resolutions, but I can't get it to work with the 40x objective. Did you try your filter on this microscope? I'm wondering if I'm missing any critical adjustment (e.g. open the diaphragm, lower the condenser, etc). Any tips are highly appreciated! :-)
I have the same microscope and can't get it to work at 40x either
I have been trying for some time
good i found this comment. I have the SW380T and have excellent results at 4x and 10x, but no luck at 40x - have all different filter sizes but no difference. Is your condensor perfectly centered? On the Swift there are no centering screws.
Do you know how to make a diy specimen slide?
They sell darkfield condenser separately. They want you to buy that.
Can you send me a link to a proper darkfield filter for a swift sw380t ? (on german amazonsite would be optimal
this is great, but i have a problem. darkfield only works using the 15x eyepiece + 5x Objective ( w a L101 that has 5X, 10X, 45X(s) +5, 10X, 15X. Eyepieces: total 675X,) followed your exact specs. making the dark circle bigger does not work... is it the mirror ilumination? ( using ext ligth source) sorry 4 the bad english!
Thank you! I was actually really wondering about this. Gonna go try.
I was wondering, what is the movable condenser on the SW380B for? When might you want to move it?
It's very beautiful. Is it not possible merely to tape a coin or thin washer onto a cheap blue filter or rigid plastic cutout?
What if your microscope does not have a filter holder? Mine is low cost #150 type with a rotating ring of different size apertures.
I know these. The apertures are too large anyway to make a difference. In this case you can not do darkfield. You can attach other filters there (polarization, color etc). or a cut-up credit card (etc) with a larger pin hole. The aperture is then very small (dark) but the depth of field goes up greatly. Off-center pinhole might give you oblique, but not sure.
Sir plz send a video on cleaning lens
Is darkfield the same as grayfield?
hi, do you have any suggestion on how to enable dark field at 400x magnification? I have the swift SW380T, but somehow i cant get it to work. Best filter size for mine is actually 1,3 mm - 1,5 was a little too large (gave a dark brown blob in the center). Many thanks!
The filter size should be much larger, about 15mm for the 40x objective (it must be even larger). Watch this video: ua-cam.com/video/6hh5ZyU35yc/v-deo.html
@@MicrobehunterMicroscopy thanks! Did you get it to work for the 40x objective? It doesnt seem the condensor size/lens combination allows for it
@@roelmattheeussen1117 switch to 10x objective and move the condenser until it appears brightest and then the switch back to 40x
😁😁😁👌genius, THANK YOU!
The diameter of the center black disk will depend upon the aperture of the objective. One cannot use one size disk for all objectives
After watching your videos I just bought this swift 380t from Amazon and I looking to 3d print a filter like the one u got any link to download the STL?
ua-cam.com/video/UgT52XoQGQQ/v-deo.html
you can find the the download links in the description of this video :)
I have a Euromex BioBlue Lab, with halogen light. Anythingh I can try to mimic darkdfield? Or is it only the blue filter?
Oh, wait. 3 W NeoLEDTM illumination . Cool I can use this. Many thanks for sharing. I will try.
WoW, that is a great info. Thank you, Can you help me what kind of Microscope and techniks do i hav eto use for viewing parazites in my blood. In my case i am a borrelia infected. Lyme sick.
I tried sticking my finger over the light, on a Swift 380 and got some good dark field/contrast effects. See it here: ua-cam.com/video/_MXBhgWcIjY/v-deo.html
Awesome! I have a SW380T but can't achieve this results. I tried to manually make darkfield filters but they are far from perfect. Did you change the lighting?
Thanks for info I really like your channel have a request can you review the vabiooth microscopes?
That is way too cool. I have an Amscope T490B-DK scope and most recently purchased their optional non-turret version of a PC (Phase Contrast) Kit for $300 to convert to and experiment with that lighting technique. Now I have both darkfield/PC lighting options to work with! 👍
Thanks for the video, I just made one but using a piece of electrical tape for the black dot, worked like a charm! It really is quite nice for 40x magnification.
I did the same thing, same microscope but everything is dark…. I opened the iris diaphragm, and light to max, plus the condenser was all the way up….
Hi! Can you recommend a good microscope to get to try this? Not too expensive....
Do you think doing darkfield this way would work for live blood analysis?
Hi thanks for video find very entertaining. Can you or someone help me please.. upgraded old microscope to modern one, will a RGB led light source be a good idea, in order to for example control colour source? I believe this is different to dark field?
I plan to get out my old wiring and substitute for usb rgb led light source that is controlled led via app/phone.
Kind regards
:)
Tal vez alguien sabe como centrar el condensador en este microscopio Swift 380B
Can this microscope be converted to phased contrast?
Would it be at all possible to add a or make a dark field filter for the telmu microscope or no ? If so how would I do that?
Great video! Thanks!
Super İdea . Thank you .
Thank you! I learn something new every single video. 😊
🌿
Sir i want to make a lab in my home sir so can can u give me some tips
Awesome.Thanks...
Thank you king!!!!!!
I love your chenel
You can move the place where you put the filter, like in 05:24, turn on the light and make some adjustments until you see a shadow in the slide. Will work like a filter. Somebody know how to make this tecnique work in high magnifications?
Only 4x and 10x
Ican't set 40x in dark field
thanks
Thank you !
THANK YOU!
Thank you!
Hi Oliver this item is not available in India so please suggest a different microscope
Swift , Omax , and amscope are all basically the same in terms of quality hope u can find one of those brands for sale
@@matgggg55 thanks bro
New sub here, THANK YOU SO MUCH for making this vid!!!
Masha Allah