Just binge watched all your videos. I really appreciate your scientific and methodical approach to mushroom cultivation. And I am so grateful for someone like you investing tremendous amount of effort and time to share these valuable knowledge. What I thought would've been a fun summer project growing shiitake have turned into a daily obsession of mine. There are so much to learn and so much yet to be discovered! Thanks Gary!
Fun! Thanks for sharing. I just saw someone who’d isolated a really funky looking phenotype and really had no idea what that meant. I appreciate your videos and I’ve been looking at mychorhizal stuff for my 2020 apocalypse garden... actually what led me to fungi in the first place. Really looking forward to what you’re bringing to fungi and gardening
Great explanation! It’s been such a cool journey integrating my lessons from mycology and incorporating them into academia and the study of insect pathogen fungi.
Thank you Gary. You don't know me but you have taught me loads already through your videos 👍 I am new to Mycology and your channel is a wealth of knowledge. Great work, Mush love from the UK
We don’t deserve you or the university level education you provide us with. Thank you! Would you have any interest in working on a collab project with a couple others in the mushroom community?
You should hydride a azurescens with something large like Ecuador strain. Or just normal cubes but either way it would be a verry nice thing. I know their food sources are different but that’s what would make this so interesting!
Thank you, your videos are amazing. Im just getting confused here a little. You say that to get a consistent result you hold onto the 1st gen that you select. What is 1st gen? Any gen that came from spores? I literally think about the very 1st mushroom patch ever, lol, that's not helpful, haha. Jokes aside, I thought that no mater the selection or breed of the previous gen, spores will always have a clean slate and anything is possible because it has diversity that then you select from? Also, you say that strains loose vigour through cloning. But then how do I hold on to the same gen? Cloning is when you grow mycelium from mushroom tissue, but also when you share grain spawn and liquid cultures, right? Or the latter grain/lc methods are what you mean by holding on to a strain? Sorry for stupid tangled questions. I feel I have all the pieces, just need to place it right..
yes one key aspect is using slants for long term storage and refrigerating or using super cool temperatures and glycol to store in cryo freezers. slowing down replication to preserve the genome as much as possible - hope that helps makes things clearer!
what approach for hybridizing are you planning to use I'm planning to try hybridizing of different phenotypes by basically using two different spore samples in my cloning process
Can you please do a video on senescence of mycelium and whether agar to agar, for example, is cloning or just an "extension of growth" of the isolated genetics. It seems illogical to me that there would be a small limit to how many times mycelium can be transferred as, to my understanding, it is not the same as cloning. What I have read about it it seems the only reason it enters senescence is due to toxic DNA and mitochondrial breakdown and is a genetic flaw within the isolation itself and not the amount of tranfers. I don't know much about biology but this is just presumptuous logic. Would love your thoughts on this. Cheers.
Do you have a video that explains how to isolate a phenotype from a culture without sophisticated laboratory testing and equipment? What do you think is the best way for the home grower with only a very basic myco-lab (e.g., microscope, LFH) can achieve the best genotype of the species when starting from a spore? Lot of videos out that show you how to make a selected rhizomorphic culture but I haven’t seen any explain why we should do that and what it means in cultivation.
Gary, how to do clone a culture right without senescence ? How people keep a strain for so long without problems? There's no video about that. Mushlove
bought 10ml of lions mane. 7days ago, i made out of it: 10 petri dishes with single drop, nothing. 2x 2ml into 750ml millet grainspawn, nothing. 3 x 750ml liquid culture with the rest. One of them no grow(bits are floating), 2nd minimal grow, 3rd look created a single line spiral no network, may contaminated by something. So gonna have to try again. But the shitake i bought from the same guy colonize like crazy, compare to the one i already had. So genetic definitely make observable difference.
the lions mane culture I have is wispy on agar and may not look like much until it fruits - that being said it could have been damaged during shipping or it tends to clump in the syringe. It’s best to vortex it first or use the whole syringe on a grain jar to get it going. Message me on ETSY and I can send a fresh one Tuesday I have been using the same Lc all year and getting good fruits.
it may or may not - it depends if the mycelium mutates - my personal opinion is that it can randomly mutate from physical stress like cutting, but some strains are more tolerant than others
senescence is only a thing with cordy. with gourmet species it's not an issue if you feed the myc different food cycles (different grains, different agar recipes)
It may be prolonged with these methods yes, but all strains eventually senesce - one way or another the G1 will always be the strongest unless a random mutation occurs along the way that selects for a desired trait. It’s just nature
I recognise you always breathing very hard. Try no to spell long phrases between breathing air. Your lungs seem underdeveloped for this. You may have formaldehyde deposits in your lungs. You could develop some issues soon, depends how did you handle contact with smoke, spores, dusts, asthma, chemicals, vapours, gases. Check your lungs in 3 different clinics.
I appreciate your concern I usually do these videos in one take between inoculation batches and Im very active - I have good doctors and am in fact healthy and can run miles at a time without issues - mushroom farming is a labor of love and filming is above and beyond so I do my best to get the info out there 🙏🏻
I'm not trying to tell you how to do your job because both of us are from good old-fashioned piece of shit New York I'm like 40 minutes away from Buffalo buddy but for real I can feel your nervousness and this video every time you take a deep breath shit freaks me out for some reason and then you looking down to the camera idk 😐 bro relax it's okay
thanks for the feedback It’s actually after I got done working out I often get ideas when I work out and film them in one take so I don’t forget but yes I agree it wasn’t ideal
You're videos are extremely helpful and have way more information than most. Thanks for blessing us with your knowledge!
MUSHLOVE
Just binge watched all your videos. I really appreciate your scientific and methodical approach to mushroom cultivation. And I am so grateful for someone like you investing tremendous amount of effort and time to share these valuable knowledge.
What I thought would've been a fun summer project growing shiitake have turned into a daily obsession of mine. There are so much to learn and so much yet to be discovered! Thanks Gary!
thanks for watching and following along!
Fun! Thanks for sharing. I just saw someone who’d isolated a really funky looking phenotype and really had no idea what that meant. I appreciate your videos and I’ve been looking at mychorhizal stuff for my 2020 apocalypse garden... actually what led me to fungi in the first place. Really looking forward to what you’re bringing to fungi and gardening
The more you know 🌈
Excellent Information Gary🍄❤️🍄
Great explanation! It’s been such a cool journey integrating my lessons from mycology and incorporating them into academia and the study of insect pathogen fungi.
@@michaelwelsh9700 thanks for watching and following along!
Thank you Gary. You don't know me but you have taught me loads already through your videos 👍 I am new to Mycology and your channel is a wealth of knowledge. Great work,
Mush love from the UK
Thank you so much for your hard work on breeding new varieties
I'm one of your biggest fans man. Thank you so much!
thanks for watching and following along! MUSHLOVE
Love your work
Very interesting information. Thanks for posting Gary!
Excellent. There is a book in your future, Gary.
You are not a farmer, actually you are a scientific ! Great information, thanks for sharing ;)
We don’t deserve you or the university level education you provide us with. Thank you! Would you have any interest in working on a collab project with a couple others in the mushroom community?
thanks for watching! Sure send some more info to my email freshfromthefarmfungi@gmail.com I will see how I can help
You should hydride a azurescens with something large like Ecuador strain. Or just normal cubes but either way it would be a verry nice thing. I know their food sources are different but that’s what would make this so interesting!
that would be an interesting combination indeed
I love fresh caps stuff but if I may Yoshi Amono does a lot of those exact types of breeding as well with a bunch of different actives.
excelent video
De lujo, que buen video 💯🦅
how can i get a better genetic out of a lios mane spore syringe using agar? What caracteristics of the micelium should I pick or look for ? Thanks
that's really valuable information thanks a lot
Thank you, your videos are amazing. Im just getting confused here a little. You say that to get a consistent result you hold onto the 1st gen that you select. What is 1st gen? Any gen that came from spores? I literally think about the very 1st mushroom patch ever, lol, that's not helpful, haha. Jokes aside, I thought that no mater the selection or breed of the previous gen, spores will always have a clean slate and anything is possible because it has diversity that then you select from? Also, you say that strains loose vigour through cloning. But then how do I hold on to the same gen? Cloning is when you grow mycelium from mushroom tissue, but also when you share grain spawn and liquid cultures, right? Or the latter grain/lc methods are what you mean by holding on to a strain? Sorry for stupid tangled questions. I feel I have all the pieces, just need to place it right..
yes one key aspect is using slants for long term storage and refrigerating or using super cool temperatures and glycol to store in cryo freezers. slowing down replication to preserve the genome as much as possible - hope that helps makes things clearer!
what approach for hybridizing are you planning to use I'm planning to try hybridizing of different phenotypes by basically using two different spore samples in my cloning process
very informative channel!
Can you please do a video on senescence of mycelium and whether agar to agar, for example, is cloning or just an "extension of growth" of the isolated genetics.
It seems illogical to me that there would be a small limit to how many times mycelium can be transferred as, to my understanding, it is not the same as cloning. What I have read about it it seems the only reason it enters senescence is due to toxic DNA and mitochondrial breakdown and is a genetic flaw within the isolation itself and not the amount of tranfers.
I don't know much about biology but this is just presumptuous logic. Would love your thoughts on this.
Cheers.
it depends on the species and even the phenotype - some strains last years and years and some
degrade quickly.
What about explaining an agrarian permaculture
Do I get the snake venom from the snake oil salesman? LOL
No
@@FreshfromtheFarmFungi lol. Do you have any new videos?
Do you have a video that explains how to isolate a phenotype from a culture without sophisticated laboratory testing and equipment? What do you think is the best way for the home grower with only a very basic myco-lab (e.g., microscope, LFH)
can achieve the best genotype of the species when starting from a spore? Lot of videos out that show you how to make a selected rhizomorphic culture but I haven’t seen any explain why we should do that and what it means in cultivation.
ua-cam.com/video/9Y81n4LGtLE/v-deo.html ua-cam.com/video/1oqchhQbxBA/v-deo.html these two videos may touch on this subject
Gary, how to do clone a culture right without senescence ? How people keep a strain for so long without problems? There's no video about that. Mushlove
it’s best to store on slants and take from the mother culture watch the video on slants for long term storage ua-cam.com/video/FTbHO2ql8X0/v-deo.html
Thank you!
Your channel is exactly the kind of channel i would create. Simple, friendly, informative
Snake venom forces crossing over??
I am wondering is it possible to hybridize reishi with turkey tail mushrooms?
You would need something like rattlesanke venom to break down the cell wals of both spiecies just enough to allow them to repair and hybridize
What happened if we make too much of agar transfers?
watch this video ua-cam.com/video/1EAewscroPc/v-deo.htmlsi=PDApYacABw6jfmtU
bought 10ml of lions mane. 7days ago, i made out of it: 10 petri dishes with single drop, nothing. 2x 2ml into 750ml millet grainspawn, nothing. 3 x 750ml liquid culture with the rest. One of them no grow(bits are floating), 2nd minimal grow, 3rd look created a single line spiral no network, may contaminated by something. So gonna have to try again. But the shitake i bought from the same guy colonize like crazy, compare to the one i already had. So genetic definitely make observable difference.
the lions mane culture I have is wispy on agar and may not look like much until it fruits - that being said it could have been damaged during shipping or it tends to clump in the syringe. It’s best to vortex it first or use the whole syringe on a grain jar to get it going. Message me on ETSY and I can send a fresh one Tuesday I have been using the same Lc all year and getting good fruits.
@@FreshfromtheFarmFungi from EUrope dont have much faith it will get here alive + not sure how is it with customs BS :(
What if you mix syringes?
only a few exceptions could mate from Dikaryotic to monokaryons spontaneously otherwise they will compete with eachother
@@FreshfromtheFarmFungi
So it would be a waste
does genotype degradation happen when cloning
it may or may not - it depends if the mycelium mutates - my personal opinion is that it can randomly mutate from physical stress like cutting, but some strains are more tolerant than others
if I combine the liquid cultures from two different strains will it result in a hybrid???
no they will compete. They need to be genetically manipulated or start from spore
@@FreshfromtheFarmFungi which begs the question of how do you genetically manipulate the mothers
1:58
senescence is only a thing with cordy. with gourmet species it's not an issue if you feed the myc different food cycles (different grains, different agar recipes)
It may be prolonged with these methods yes, but all strains eventually senesce - one way or another the G1 will always be the strongest unless a random mutation occurs along the way that selects for a desired trait. It’s just nature
@@FreshfromtheFarmFungi thanks for replying :)
dont forget breathing my guy
I recognise you always breathing very hard. Try no to spell long phrases between breathing air. Your lungs seem underdeveloped for this. You may have formaldehyde deposits in your lungs. You could develop some issues soon, depends how did you handle contact with smoke, spores, dusts, asthma, chemicals, vapours, gases. Check your lungs in 3 different clinics.
I appreciate your concern I usually do these videos in one take between inoculation batches and Im very active - I have good doctors and am in fact healthy and can run miles at a time without issues - mushroom farming is a labor of love and filming is above and beyond so I do my best to get the info out there 🙏🏻
I'm not trying to tell you how to do your job because both of us are from good old-fashioned piece of shit New York I'm like 40 minutes away from Buffalo buddy but for real I can feel your nervousness and this video every time you take a deep breath shit freaks me out for some reason and then you looking down to the camera idk 😐 bro relax it's okay
thanks for the feedback It’s actually after I got done working out I often get ideas when I work out and film them
in one take so I don’t forget but yes I agree it wasn’t ideal