Ma'am these videos are just a blessing for all the students. I've fallen in love with learning all because of you. You were born to become a teacher. You were born to do this ❤❤
Attention please!! Mam have miss few topics from ncert she didn't talk about 👉chromogenic substrate 👉Vector for cloning site in plants and animals example agrobacteium tumifaciens 👉about disarmed pathogens 👉 about thermus aquaticus So please look these from your ncert
0:00 Introduction 0:51 Biotechnology 2:41 Basis of Biotechnology 7:03 Principles of Biotechnology 9:21 Why Genetic Engineering? 11:07 Disadvantages of traditional Hybridization 12:31 Introducing Genetic Engineering 13:34 Genetic engineering 20:46 Genetic modification of an Organism 21:53 Important tools for Genetic Engineering 23:20 Restriction Enzymes 25:13 Recognition Sequence of Restriction Endonucleases 30:33 Naming of Restriction Endonucleases 33:14 Working of Restriction Endonucleases 36:26 Step-wise working of Restriction Endonucleases 36:26 Recombinant DNA (rDNA) formation 40:23 How to separate DNA fragments? 44:08 Elution 46:48 Vectors 48:08 Cloning Vectors: Examples 48:54 Cloning into a Vector 49:35 Feature in Cloning vector: Origin of Replication 52:53 Feature in Cloning vector: Restriction sites 54:09 Feature in Cloning vector: Selectable marker 56:33 Transformation 1:02:23 Insertional Inactivation 1:06:05 Competent Host 1:09:09 Techniques to insert rDNA into host cell 1:09:15 Gene Gun method 1:09:41 Electroporation method 1:10:53 Micropipette method 1:11:32 Genetic Engineering: Steps 1:12:59 Isolation of DNA 1:13:50 Enzymes involved in Isolation of DNA 1:15:17 Cutting of DNA at specific locations 1:16:28 Amplification of Gene of Interest 1:17:20 Polymerase Chain Reaction 1:20:25 Polymerase Chain Reaction: Steps 1:28:04 Insertion of rDNA into host cell 1:29:11 The Foreign Gene Product 1:30:19 Bioreactors 1:31:17 Different Bioreactors 1:32:27 Downstream Processing
1:05:40 amazing explanation of selectable marker i was never able to understand this topic so well thank you ma'am for your great effort and amazing explanation ❤🙏🏼🌼
I thought that this chal is too difficult but after looking and learning from her teaching all my concepts are crystal and clear, All the BEST to all the bio students ❤
She's super women yrr......❤ Mtlb itne hard topics ko bhi itnee sahi tarike se crystal clear krna har kisi ki baat ni hai....hatts off uhh mam🫡 mene puri 12th sirf aapke hi lecture ko dekh kr paper diye hai and mere sare paper bhot ache gye hai....and all thanks too uhhh❤❤
I have to say this... I tried reading this chapter 2-3 times.. before my class test and pre board..but after your lecture today I understood this chapter in such a way that.. I'm pretty sure I'll never forget all these... thank you so much Roshni maam..you have really become the only roshni ki ummid in my life And I also wants..to thank you for saving my chemistry paper you are the best best teacher of my life.. I have ever seen and you will be the example for the best teacher for me...at every stage of my life. Thank you ❤️
Timestamps: 0:00 Introduction 0:51 Biotechnology 2:41 Basis of Biotechnology 7:03 Principles of Biotechnology 9:21 Why Genetic Engineering? 11:07 Disadvantages of traditional Hybridization 12:31 Introducing Genetic Engineering 13:34 Genetic engineering 20:46 Genetic modification of an Organism 21:53 Important tools for Genetic Engineering 23:20 Restriction Enzymes 25:13 Recognition Sequence of Restriction Endonucleases 30:33 Naming of Restriction Endonucleases 33:14 Working of Restriction Endonucleases 36:26 Step-wise working of Restriction Endonucleases 36:26 Recombinant DNA (rDNA) formation 40:23 How to separate DNA fragments? 44:08 Elution 46:48 Vectors 48:08 Cloning Vectors: Examples 48:54 Cloning into a Vector 49:35 Feature in Cloning vector: Origin of Replication 52:53 Feature in Cloning vector: Restriction sites 54:09 Feature in Cloning vector: Selectable marker 56:33 Transformation 1:02:23 Insertional Inactivation 1:06:05 Competent Host 1:09:09 Techniques to insert rDNA into host cell 1:09:15 Gene Gun method 1:09:41 Electroporation method 1:10:53 Micropipette method 1:11:32 Genetic Engineering: Steps 1:12:59 Isolation of DNA 1:13:50 Enzymes involved in Isolation of DNA 1:15:17 Cutting of DNA at specific locations 1:16:28 Amplification of Gene of Interest 1:17:20 Polymerase Chain Reaction 1:20:25 Polymerase Chain Reaction: Steps 1:28:04 Insertion of rDNA into host cell 1:29:11 The Foreign Gene Product 1:30:19 Bioreactors 1:31:17 Different Bioreactors 1:32:27 Downstream Processing
Mam me starting me itna pareshan thi ki kese hoga complete ye bio physics chemistry lekin apki wajah se kitni easily sab complete ho gya apke aane se meri zindagi me bahar aa gai❤️
Topics that are not included ; 1. Experiment of Stanley Cohen and Hebert Boyer 2.Chromogenic substrate 3. A.Tumefaciens and Retroviruses 4.Competent host (methods) - Enabling of bacteria to take up the rDNA by treating with Ca and incubation - Disarmed pathogen vectors 5. Isolation of DNA by chilled Ethanol 6. Recombinant protein 7.Continuous culture systems
1.Identification of dna with desire genes 2. Extraction of piece of dna with desire genes 3. Formation of recombinant dna 4.introduction of recombinant dna (rdna) into host 5.multiplication of rdna in host 6. Transfer of dna to progeny
Super duper amazing lecture..... thank you so much mam isse best explanation ho hi nhi skti..sb kuch ekdm clarity se bataya h apne....Paisa vasul lecture 🙏🙏
Mam thank you making these amazing lecture. I found some problem 😕 You forgot to describe chilled ethanol for isolation of pure DNA . heat shock method for inserting rDNA . At 1:18:38 If replication take place from 5'-3' then why primers attach at 3' end. I hope you will solve my doubts.
Yes mam really we need it 😢As you told in that video uploaded on Ted x Our time is also get wasted on the bus stop... Now I am a 12th appearing student ..but the concepts of 11th are not cleared..
Thank you so much ma'am this lecture was my saviour....i was struggling so much from 13th march itself,was revising my notes that were too huge and doing that I could complete only 4.5 chapters till 16 th march but now when I got your videos I stopped that laborious task and decided to go ahead with only your lectures and pyqs and not even reading the ncert anymore due to deficiency of time.... someone please suggest me if this is perfect or not
Aapke jaisi teacher kabhi nhi dekhi mai matlb aapke padhane ka style just woww you great mam jis jis chapters se mai durr bhagti thi un sb chapters se aapne meri friendship kara dii ☺️💖
Thank you mam🥺idk if you will ever read my comment but thànk you so much mam for giving us free education 🥺🥺🥺🥺 many of us can't even afford many costly tuitions and coaching but thank you mam for your hard work🥺🥺🥺🥺🥺🥺❤️❤️❤️❤️ finally we can say that "Our concepts are crystal clear" Please everyone keep supporting our Roshni mam🥺🥺❤️
Ma'am i dont have words .. i left the chapter thrice because i couldn't found any best explanation . The way you didi it is extremely mesmerizing. Thank you soo mch . Lots of love 😚❤️🫂
Timestamps: 0:00 Introduction 0:51 Biotechnology 2:41 Basis of Biotechnology 7:03 Principles of Biotechnology 9:21 Why Genetic Engineering? 11:07 Disadvantages of traditional Hybridization 12:31 Introducing Genetic Engineering 13:34 Genetic engineering 20:46 Genetic modification of an Organism 21:53 Important tools for Genetic Engineering 23:20 Restriction Enzymes 25:13 Recognition Sequence of Restriction Endonucleases 30:33 Naming of Restriction Endonucleases 33:14 Working of Restriction Endonucleases 36:26 Step-wise working of Restriction Endonucleases 36:26 Recombinant DNA (rDNA) formation 40:23 How to separate DNA fragments? 44:08 Elution 46:48 Vectors 48:08 Cloning Vectors: Examples 48:54 Cloning into a Vector 49:35 Feature in Cloning vector: Origin of Replication 52:53 Feature in Cloning vector: Restriction sites 54:09 Feature in Cloning vector: Selectable marker 56:33 Transformation 1:02:23 Insertional Inactivation 1:06:05 Competent Host 1:09:09 Techniques to insert rDNA into host cell 1:09:15 Gene Gun method 1:09:41 Electroporation method 1:10:53 Micropipette method 1:11:32 Genetic Engineering: Steps 1:12:59 Isolation of DNA 1:13:50 Enzymes involved in Isolation of DNA 1:15:17 Cutting of DNA at specific locations 1:16:28 Amplification of Gene of Interest 1:17:20 Polymerase Chain Reaction 1:20:25 Polymerase Chain Reaction: Steps 1:28:04 Insertion of rDNA into host cell 1:29:11 The Foreign Gene Product 1:30:19 Bioreactors 1:31:17 Different Bioreactors 1:32:27 Downstream Processing Copy and paste 😂😂
Ma'am these videos are just a blessing for all the students. I've fallen in love with learning all because of you.
You were born to become a teacher. You were born to do this ❤❤
❤
I strongly agree this❤😊
Absolutely correct❤
True
Frrr!!😭
"The good teacher explains.
The superior teacher demonstrates.
The great teacher inspires."
#Roshni mam is the best
😂😂😂
😂😂😂
Attention please!!
Mam have miss few topics from ncert she didn't talk about 👉chromogenic substrate
👉Vector for cloning site in plants and animals example agrobacteium tumifaciens
👉about disarmed pathogens
👉 about thermus aquaticus
So please look these from your ncert
I think it's deleted
Haa yrr
No its not@@anwishasingh7479
@@fyroboi2053In our syllabus from this chapter only these topics is in syllabus of 2024 i.e genetic engineering(Recombinant DNA Technology)
@@OwaisSoob-v7g there hasn't been an official announcement from nta for NEET 2025 don't trust other sources
0:00 Introduction
0:51 Biotechnology
2:41 Basis of Biotechnology
7:03 Principles of Biotechnology
9:21 Why Genetic Engineering?
11:07 Disadvantages of traditional Hybridization
12:31 Introducing Genetic Engineering
13:34 Genetic engineering
20:46 Genetic modification of an Organism
21:53 Important tools for Genetic Engineering
23:20 Restriction Enzymes
25:13 Recognition Sequence of Restriction Endonucleases
30:33 Naming of Restriction Endonucleases
33:14 Working of Restriction Endonucleases
36:26 Step-wise working of Restriction Endonucleases
36:26 Recombinant DNA (rDNA) formation
40:23 How to separate DNA fragments?
44:08 Elution
46:48 Vectors
48:08 Cloning Vectors: Examples
48:54 Cloning into a Vector
49:35 Feature in Cloning vector: Origin of Replication
52:53 Feature in Cloning vector: Restriction sites
54:09 Feature in Cloning vector: Selectable marker
56:33 Transformation
1:02:23 Insertional Inactivation
1:06:05 Competent Host
1:09:09 Techniques to insert rDNA into host cell
1:09:15 Gene Gun method
1:09:41 Electroporation method
1:10:53 Micropipette method
1:11:32 Genetic Engineering: Steps
1:12:59 Isolation of DNA
1:13:50 Enzymes involved in Isolation of DNA
1:15:17 Cutting of DNA at specific locations
1:16:28 Amplification of Gene of Interest
1:17:20 Polymerase Chain Reaction
1:20:25 Polymerase Chain Reaction: Steps
1:28:04 Insertion of rDNA into host cell
1:29:11 The Foreign Gene Product
1:30:19 Bioreactors
1:31:17 Different Bioreactors
1:32:27 Downstream Processing
Ligase enzymes?
Timing????
Apna time khu waste krte ho
Waiting for this chapter from the beginning of 12th class
Thank you crystal clear...
1:05:40 amazing explanation of selectable marker i was never able to understand this topic so well thank you ma'am for your great effort and amazing explanation ❤🙏🏼🌼
I thought that this chal is too difficult but after looking and learning from her teaching all my concepts are crystal and clear, All the BEST to all the bio students ❤
She's super women yrr......❤ Mtlb itne hard topics ko bhi itnee sahi tarike se crystal clear krna har kisi ki baat ni hai....hatts off uhh mam🫡 mene puri 12th sirf aapke hi lecture ko dekh kr paper diye hai and mere sare paper bhot ache gye hai....and all thanks too uhhh❤❤
@rekhabariya9430....soo true!! well said!!
Yes yes
Correct 💯
I have to say this... I tried reading this chapter 2-3 times.. before my class test and pre board..but after your lecture today I understood this chapter in such a way that.. I'm pretty sure I'll never forget all these... thank you so much Roshni maam..you have really become the only roshni ki ummid in my life
And I also wants..to thank you for saving my chemistry paper you are the best best teacher of my life.. I have ever seen and you will be the example for the best teacher for me...at every stage of my life. Thank you ❤️
Hello buddy's ❤😅😅 best luck for your exam in any year of future 😅😅😅😅😊❤❤😅😅😅
Thank you bro
In 2025😢
All the best guyzz....for board exam....i wish ki sb 90+ score kre bio mai ❤❤❤
Timestamps:
0:00 Introduction
0:51 Biotechnology
2:41 Basis of Biotechnology
7:03 Principles of Biotechnology
9:21 Why Genetic Engineering?
11:07 Disadvantages of traditional Hybridization
12:31 Introducing Genetic Engineering
13:34 Genetic engineering
20:46 Genetic modification of an Organism
21:53 Important tools for Genetic Engineering
23:20 Restriction Enzymes
25:13 Recognition Sequence of Restriction Endonucleases
30:33 Naming of Restriction Endonucleases
33:14 Working of Restriction Endonucleases
36:26 Step-wise working of Restriction Endonucleases
36:26 Recombinant DNA (rDNA) formation
40:23 How to separate DNA fragments?
44:08 Elution
46:48 Vectors
48:08 Cloning Vectors: Examples
48:54 Cloning into a Vector
49:35 Feature in Cloning vector: Origin of Replication
52:53 Feature in Cloning vector: Restriction sites
54:09 Feature in Cloning vector: Selectable marker
56:33 Transformation
1:02:23 Insertional Inactivation
1:06:05 Competent Host
1:09:09 Techniques to insert rDNA into host cell
1:09:15 Gene Gun method
1:09:41 Electroporation method
1:10:53 Micropipette method
1:11:32 Genetic Engineering: Steps
1:12:59 Isolation of DNA
1:13:50 Enzymes involved in Isolation of DNA
1:15:17 Cutting of DNA at specific locations
1:16:28 Amplification of Gene of Interest
1:17:20 Polymerase Chain Reaction
1:20:25 Polymerase Chain Reaction: Steps
1:28:04 Insertion of rDNA into host cell
1:29:11 The Foreign Gene Product
1:30:19 Bioreactors
1:31:17 Different Bioreactors
1:32:27 Downstream Processing
thanks bro/sis
Ready for 19 March attendance here
😂...phodenge bro
Yeah ...bro
Tod phod dena h😂
Log message ki baat krte h ki seen krke chod diya, yaha hum paper ko seen krke chod dete h😂😂
Baval macha denge
Mam me starting me itna pareshan thi ki kese hoga complete ye bio physics chemistry lekin apki wajah se kitni easily sab complete ho gya apke aane se meri zindagi me bahar aa gai❤️
If you find the video useful, please spread the word.
@@LearnoHubClass1112mam please aap live classes start krfo with fee. Taki hum aapko live mai doubts bool sakhay. Mgr khus padana
@@LearnoHubClass1112 ok mam
57:07 ghanta farak padta hee Mam become chad 🗿🗿🗿🗿
😂👍🏻
Topics that are not included ;
1. Experiment of Stanley Cohen and Hebert Boyer
2.Chromogenic substrate
3. A.Tumefaciens and Retroviruses
4.Competent host (methods)
- Enabling of bacteria to take up the rDNA by treating with Ca and incubation
- Disarmed pathogen vectors
5. Isolation of DNA by chilled Ethanol
6. Recombinant protein
7.Continuous culture systems
its in the text btw
Tip - take ncert with you while attending the lecture because some topics are skipped in this lecture so yahh all the best
😂😂 2025 boards
Yes 🧠😢
4 week phele se padh rha h Tu 🫥
4 week phele se padh rha h Tu 🫥
Yes😢
Which board Secondary or Higher Secondary?
That means 10th or 12th?
Tension not bro tu pass ho jayga 😁
Bagwan apko sukun deyeh❤
Bhagwan karee sunny leone jaisa patni milee aap koo
Thank you ❤
Bhagwan aapki aatma ko shanti de ❤☺️
@@aditya_yadav851ye kya maang raha hai bhagwan se 😂
Concepts hogayi crystal clear!! Thanks a lot mam..
Highly recommended lecture for this chapter ..
Thank you so much ma'am for wonderful explanation.
One of the best channel I never ever seen before thank you so much ma'am
Thank u❤❤❤❤ mam for this amazing video....may God bless u and all concepts are crystals clear😌
Me watching it at 12am clock tomorrow is my exam 😢
Anyone from2024-25 batch 😅
Love you biotechnology ❤❤🎉
Hats off to your efforts mam thanks a lot that you made our concept crystal clear before boards exams 😅🙏🤍
Every topic in deep with less time and concept = crystal clear 🌟
Mam,use same type of graphics at the time of PHYSICS,Concept hoga crystal clear
Rbse board m ho kya
I can't share video to my friends because this is masterpiece 😁😁
1.Identification of dna with desire genes
2. Extraction of piece of dna with desire genes
3. Formation of recombinant dna
4.introduction of recombinant dna (rdna) into host
5.multiplication of rdna in host
6. Transfer of dna to progeny
ye sb 12 m hai??
Hi 😂 reading now for exam
Wow
Chhotu chai lao legend aa gye 😂😂 exam ke 1 din pahle padhne 😅😂
Thank you for calling me legend 🙏
are bc kitna funny joke maardiya
Super duper amazing lecture..... thank you so much mam isse best explanation ho hi nhi skti..sb kuch ekdm clarity se bataya h apne....Paisa vasul lecture 🙏🙏
Mam thank you making these amazing lecture.
I found some problem 😕
You forgot to describe chilled ethanol for isolation of pure DNA . heat shock method for inserting rDNA .
At 1:18:38
If replication take place from 5'-3' then why primers attach at 3' end. I hope you will solve my doubts.
primer binds at template and the direction of the complementary strand 5 'to 3'
Thankyou so much, mam for explaining the chapter by making such a productive lecture and helping us at the need of hour. May God bless you🙏🙏
Mam aap allrounder ho kya 😂
Same question
25 march wle bacho kha ho💀
Yhi ha😂😂 bahut dar lag rha
Birthday h mera bro💀
Present 🙋😢
Perfectly crystal clear❤️
If you truly appreciate the efforts we put to create the content, Please Spread the word. Thanks for your support.
YOU ARE MASTER PIECE OF EDUCATION.....❤ I love you mam 😂
Very fruitful lecture thanks you so much ma'am for your efforts ❤
Ma'am you are great
You are very hardworking
The only respectable teacher which teaches all PCMB subjects. I really like it mam❤❤❤
Thanks ! If you like our videos, please Spread the word.
Mam class 11th ki one shot live class leke aaye na 😊😁
Yess mam please most required
Yes mam really we need it
😢As you told in that video uploaded on Ted x
Our time is also get wasted on the bus stop...
Now I am a 12th appearing student ..but the concepts of 11th are not cleared..
Bhai mere time pe bhi nahi thi, phir mein 12th mein aagayi aur jaa bhi rahi hu. Tu bhi aake chala jana
Thank you so much ma'am this lecture was my saviour....i was struggling so much from 13th march itself,was revising my notes that were too huge and doing that I could complete only 4.5 chapters till 16 th march but now when I got your videos I stopped that laborious task and decided to go ahead with only your lectures and pyqs and not even reading the ncert anymore due to deficiency of time.... someone please suggest me if this is perfect or not
i think that is enough but you should maybe also read one page notes or mindmaps as revision
should read NCERT
Concept hua crystal clear ❤
Aapke jaisi teacher kabhi nhi dekhi mai matlb aapke padhane ka style just woww you great mam jis jis chapters se mai durr bhagti thi un sb chapters se aapne meri friendship kara dii ☺️💖
22:45 22:49
Like karo salo... Isko like nahi dohge.. Tho kisseh dogeh?
Thank you mam🥺idk if you will ever read my comment but thànk you so much mam for giving us free education 🥺🥺🥺🥺 many of us can't even afford many costly tuitions and coaching but thank you mam for your hard work🥺🥺🥺🥺🥺🥺❤️❤️❤️❤️ finally we can say that "Our concepts are crystal clear"
Please everyone keep supporting our Roshni mam🥺🥺❤️
Yes i agree🥺🎀
If you really like the classes, please spread the word.
Thank you mam for your recognition 🥺❤️@@LearnoHubClass1112
Ma'am i dont have words .. i left the chapter thrice because i couldn't found any best explanation . The way you didi it is extremely mesmerizing. Thank you soo mch . Lots of love 😚❤️🫂
If you really liked the video, Do not forget to Spread the word. Lets together Promote Free education.
Kal paper hai 40 syllabus complete bachaa hai 😂
Same here dear 😢
Same here dear 😢😢
@@vidhiyadav6836 don't wary We will complete or if less time
kv education help fast to complete syllabus my favourite for revision
*Free hai par best hai* ✓
*Thank you so much for this ma'am :)*
Concept hua crystal clear ❤...
Thank you so much, Roshni Mam.
What a amazing lecture from this lecture first time I understand biotechnology ❤❤
Lallan top madam gi, from bihar😊😊😊
19 march 2:45 Am pe chal raha hai 😂😂 kuchch ghante baad ecam hai
Mam really your teaching is work like as energy drink 🍷 for all students you are best❤️
Ya correct 😊
Started on 9 oct hope i complete my lecture today ❤️ let's see
Concept hua crystal clear .......😍♥️👍
19 ko fordenge 🔥
In my opinion, Jo koi chapter first vaari padhne aaya h uske liye best h 👍🏻💯✔️....
Gorgeous explosion 💥
❤❤❤
Thank you so much mam ❤
Ready for tomorrow's exam ❤❤😊😊all the concepts are crystal clear ✨
😳
Tomorrow is exam starting my study today
Same bro😅
57:08 I sure that anyone can't notice this
Ghanta 😂
Concept hua Crystal clear....😊😊
Thank u so much mam❤
SHARE if you like our content!
@LearnoHubClass1👍🏻😊❤
The best part about lecture is it has 3D pictures n becoz of this it helps a lot to clear concept....👍👍
BEST OF LUCK FOR YOUR EXAM HOPE YOU DO BEST 🔥🔥🔥
39:15 what if the vector\plasmid does not contain the GAATTC gene ?
That's not possible.
Concept hua crystal clear thank u mam✌️🥰👍
1:30:47 💀 nice isliye awaj hta diya 👍
Shyd jute cell bol diya 😂
@@sanjayedits human cell use karke useful products banane ki baat chalri 😂
Maam class 12 bio ka playlist bnadijiye for 2024 😢
Already hai bhai
This is enough for boards suggest me
Yes
🙏 maam hum apke purane student hai , pehle padhte the 2018-19 me ,abhi engineering last year me hu , thankyou maam 🙏
Mam your channel name is learnohub and you are superb🎉🎉🎉🎉
Thank you mam ♥️
All concepts crystal clear ✨
Ready for 28 march (rbse) attendance here 🗿🥂
besttttesttt video on youtube for clearing confusion on selectable marker♥😍
Crystal clear mam ❤
If you truly appreciate the efforts we put to create the content, Please Spread the word. Thanks for your support.
Roshni mam is worthy of more recognition ❤❤❤❤❤ love from west bengal
Absolutely 💯
Miss u made me feel like a kindergarden student again 😊😊very nice
Concept huya crystal clear mam ....Thank you so much for uploading one shots of all chapters of 12 class .......Thanks a lot mam❤❤❤
11:09 zebroid fifth leg is visible 💀
Concept was crystal and clear mam❤
My birthday at 19 😢 at last exam frrr
💀
Great explaination mam loved the video thank you❤
Keep watching. Please support us by spreading the word, so that we continue to make more such learning content.
Suree mam🤍🤍
concept hua crystal clear♥♥♥♥♥
Best video for revision
Achyaaan
Are you guys ready for 25 March 2025🎉🔥❤ Wishing you all may get💯!!
Attendance here👇🏼
concept hua crystal clear😀
Now my concept is crystal clear 💗💥
Your video was very helpful for my board exam 💗 thank you so much mam❤️✨
Timestamps:
0:00 Introduction
0:51 Biotechnology
2:41 Basis of Biotechnology
7:03 Principles of Biotechnology
9:21 Why Genetic Engineering?
11:07 Disadvantages of traditional Hybridization
12:31 Introducing Genetic Engineering
13:34 Genetic engineering
20:46 Genetic modification of an Organism
21:53 Important tools for Genetic Engineering
23:20 Restriction Enzymes
25:13 Recognition Sequence of Restriction Endonucleases
30:33 Naming of Restriction Endonucleases
33:14 Working of Restriction Endonucleases
36:26 Step-wise working of Restriction Endonucleases
36:26 Recombinant DNA (rDNA) formation
40:23 How to separate DNA fragments?
44:08 Elution
46:48 Vectors
48:08 Cloning Vectors: Examples
48:54 Cloning into a Vector
49:35 Feature in Cloning vector: Origin of Replication
52:53 Feature in Cloning vector: Restriction sites
54:09 Feature in Cloning vector: Selectable marker
56:33 Transformation
1:02:23 Insertional Inactivation
1:06:05 Competent Host
1:09:09 Techniques to insert rDNA into host cell
1:09:15 Gene Gun method
1:09:41 Electroporation method
1:10:53 Micropipette method
1:11:32 Genetic Engineering: Steps
1:12:59 Isolation of DNA
1:13:50 Enzymes involved in Isolation of DNA
1:15:17 Cutting of DNA at specific locations
1:16:28 Amplification of Gene of Interest
1:17:20 Polymerase Chain Reaction
1:20:25 Polymerase Chain Reaction: Steps
1:28:04 Insertion of rDNA into host cell
1:29:11 The Foreign Gene Product
1:30:19 Bioreactors
1:31:17 Different Bioreactors
1:32:27 Downstream Processing
Copy and paste 😂😂
TNX 😊 recall krne me bhot help hua😅
You forgot bahar se kela andar se corn 20:46😅😅
Vote for Class 11 and 12 due chapters..! /
Thank you for amazing explanation❤
Is this sufficient for boards?
Mam this lecture is amazingly defined by you
But I'mma having doubt that if its useful for 2025 boards I mean 2024-2025
Mam 11th ka bhi isi tarah banaiye please 🙏
Thank you so much ma'am ❣️
Most welcome 😊
Concept huye crystal clear my sweet mam❤ me 12th aapse hi clear krungi ur prac krungi.
Ky padhate ho mam ap😍 tenson hi khtm kr dete ho
Concept is crystal clear
Mam please make biology videos for class 11 🙏🙏