I just don't know how come the Primers meting temperature would have an impact on template DNA (dsDNA)? I mean primers should be single strand DNA right?
Knowing the melting temperature of a primer makes it possible to know around which temperature range the primers manage to anneal on the denatured matrix (single strand).
영상 너무 잘 보았습니다!! 감사합니다!
Great explanation!! Thank you very much!
My primer is p1/p7 for first round of pcr. And r16f2n/r16r2 for 2nd round of pcr. For nested pcr, what should i do that my dna band result will come?
Mam my primer set have basic melting temperature 56, i want to set gradient PCR can you tell me about temperature range for PCR
Hello, how can I calculate the hybridization temperature?
Thank yo very much❤✨
I just don't know how come the Primers meting temperature would have an impact on template DNA (dsDNA)? I mean primers should be single strand DNA right?
Knowing the melting temperature of a primer makes it possible to know around which temperature range the primers manage to anneal on the denatured matrix (single strand).
I lovvve it. Thank you!
Where is the nearest neighbor method?
If the tm of forward and reverse primer is different then what should we do
So does this mean the longer the sequence the greater the melting point?
+Carl Onwochei yes correct
Nearest neighbor?
How can we find the annealing temperature?
Its calculated by subtracting 5 degrees from the lowest temperature among the forward or reverse melting temperatures.
Plz give melting and annealing of DNA in hindi
I am really sorry Chandrakant, It is really difficult at the moment to provide it in hindi because I do not know any native speaker of hindi.
your math is off with your log in the Howley equation