Agar & Cultures Part 2: MEA and Antibiotic Agar remix
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- Опубліковано 14 гру 2021
- In the next video - we talk about expanding that basic MEA recipe and core idea and expand into antibiotic/charcoal or even hydrogen peroxide! Why use antibiotic agar? Why not?
#mushrooms #fungi #humblefungus #mycology #fungiculture - Розваги
Really awesome series man, happy your doing this for the mush community. In the future would you do a series on sectoring and isolating cultures? I know how but it would be useful to some who dont know and there is not a lot of videos on the subject on youtube.
I used some charcoal in my last batch of agar, around 2 dozen plates and no contams as of yet.
Jesse! I just wanted to thank you you have helped me out immensely with my mycological endeavours!
Great to see you exploring this topic outside the normal discussion themes. Thank you.
Not gonna lie the kubernetes logo makes me trust him even more. Keep up the good work, I love your videos!
Your are excellent at explaining things, thank you for your knowledge.
Hi! Been loving your videos lately and you are my main source of information as of now. I think the bigger science related channels are more concerned about video production quality, than quality of information and the so called "truths" of different sciences like mycology, here. I started to grow mushrooms ~8months ago - and it's funny how just as I am naturally progressing through the art of mycology and moving onto agar - you post a video ;D Bought my first pack of Agar Agar a week ago unaware of the fact that a new "Humble Fungus' Super Cool Nerd Shit" -series was about to be released. Ha, funny coincidence, but lucky me!
Anyway, I rarely comment cuz I never have nothing useful to say, but this time I have a question... since I've been swallowing up information about agar the past few days and that's the only thing I can concentrate on;
You mentioned accidental creation of antibiotic resistant strains, like strep-whatever-coccus(did I get it right?? maybe i did), so could you elaborate further on that subject? How easy/hard is it to create harmful bacteria and/or other sorts of actually dangerous stuff? Most of us probably don't have clean laboratory conditions, and my current setup is so ADHD-engineered, that only people like you could understand. maybe, I dunno, now I'm wayyyy off track anyway.
You seem well educated about the subject matter of mycology and I'm just a beginner who's only information source is the internet. Many things can go wrong along the way, since I'm not teaching myself these things structurally, or in the right order, that might be the only drawback in the act self-learning different stuff off of internet and books. I'm not the most patient out there, but i am precise when it comes to things i'm obsessed about hahaha
Okay, hope ye well fellow human being. Love from Finland!
That opening had me laughing 😂
You are fucking a God! Thank you! Out of ALL the months of me researching agar recipies, you taught me more in this one video that all of those previous videos put together! Thank you! Thank you! Thank you!
Bro youre like the Tony Starks of mushrooms 🍄
Thanks
Very interesting video, i have been taking grief in mycology groups for trying out activated charcoal on agar, people say it does absolutely nothing besides adds color. They also say that peroxide would just kill the mycelium. I have spores on . activated charcoal agar now and they are growing slowly but they are growing.
Hey brother, I have really been enjoying your videos. My issue is triche😡 tends to come from my sterile tek innoculating bags or jars. I'm not a beginner but not a veteran either. Would you think building a plastic tent and having a SAB inside of that would help? Like you could wipe down the inside of the plastic walls and have much less air flow?
Loving these videos man! I'm curious if using charcoal or hydrogen peroxide would be a better option when using a multi-score syringe that has been purchased or a spore print to an agar plate?
I would say so. Anything that you can do to prevent contam is what you want to do. I usually use charcoal plates when I'm germinating and I definitely notice a difference between plates without.
Me and my ol man use an auger for ice fishin.
nice!! when do you add the h2o2, before or after sterelization?
Before!
@@PracticalMyco Hi, isn't the H2O2 decomposing above 60°C ? I always put it after sterilization for this reason, but maybe do I miss something?
I wonder how many people will get the Julia Child/ Mrs. Doubtfire reference.
Zero point one millennials
Is not grain water better for agar so the mycelium remembers it for grain jars ?
can i use malt extract only i couldnt find LME
hi, are you sure about using hydrogen peroxide before sterilization?
Not auger it's agar with the A sound in the first a and ah sound for the second making an arrr sound at the end
What percentage is the H2O2? Mine is 13% but seen it anywhere between 3-20%
He said 3 %
Hi, thanks for the video.
But don't hydrogen peroxide break up in high heat and become normal water?
It does not: www.ncbi.nlm.nih.gov/pmc/articles/PMC4249246/
@@PracticalMyco you are a hero! I'm assuming that spores can't colonies hydrogen peroxide agar? Or are going to turn my world upside down?
Can you use h202 in a liquid culture?
Liquid cultures are easy to create and multiply in number; so, try it out with 2 identical cultures except one has 3% H2O2 at the concentration of 100 ml in 750 ml of H2O, and see what happens. I've heard of a small fish tank compressor bubbling with a filtered (syringe filter) tube in a liquid culture jar, and increased the growth rate of the mycelium a lot. 🥸