Hey, I couldn't find a proper animation video for the explanation of whole concept and working pf Real time PCR using Cyber green.. I think there is a need of good animation videos of the same.
"The 3' end should contain no more than 2 G or C bases to avoid specificity issues." Is this correct? I think the 3' end should have MORE G or C bases to form GC clamps that increase specificity.
Thanks Joshua for your question. If you are uncertain of what temperature to use for your annealing temperature, a temperature gradient is a great way to test what optimal annealing temperature to use. However, if you already know what annealing temperature to use, a temperature gradient is not necessary. For additional technical support, please contact us at thermofisher.com/askaquestion. Thank you!
Thank you
You're welcome, we are glad the video was helpful.
Thank you so much, I am just learning how to use the ThermoFisher thermo cycle with Sybr and next week with taqman
This was very helpman, thanks TaqMan!
Thanks for watching!
Hey, I couldn't find a proper animation video for the explanation of whole concept and working pf Real time PCR using Cyber green.. I think there is a need of good animation videos of the same.
"The 3' end should contain no more than 2 G or C bases to avoid specificity issues." Is this correct? I think the 3' end should have MORE G or C bases to form GC clamps that increase specificity.
Thank you .excellent.
how to optimize primers ?
Nice drawings
Should I run temperature gradients?
Thanks Joshua for your question.
If you are uncertain of what temperature to use for your annealing temperature, a temperature gradient is a great way to test what optimal annealing temperature to use. However, if you already know what annealing temperature to use, a temperature gradient is not necessary.
For additional technical support, please contact us at thermofisher.com/askaquestion. Thank you!
Johnny Knoxville?
ryan reynolds?