Thank you for the information. How many standard curves are necessary during Sybr green assay? is it necessary to create standard curve for each gene? Or can I use only one standard curve which created with housekeeping gene for other gene expression analysis?
Thank you for the information. How many standard curves are necessary during Sybr green assay? is it necessary to create standard curve for each gene? Or can I use only one standard curve which created with housekeeping gene for other gene expression analysis?