How to pipette correctly - a short step-by-step introduction into proper pipetting
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- Опубліковано 26 лют 2017
- #pipetting #tutorial #labhacks
This three-minute video improves your pipetting skills and secures a homogenous pipetting technique in your lab when all lab members follow these easy tips. Your assays will be more reproducible and reliable without depending on one person doing the experiment. We will show each important step in pipetting from the operation of the buttons to dispensing of the liquid. We give valuable information on each handling move and explain in detail all critical points during pipetting. You will learn about the correct aspiration angle and immersion depth and receive reasons why these considerations are of great importance. We clarify the term and practice of pre-wetting by showing how to do it. In the last step of pipetting you will learn about the appropriate dispensing angle and performance of blow-out to achieve most reliable and reproducible results.
DISCOVER THE EPPENDORF PIPETTE PORTFOLIO & TIPS:
👉 Our pipettes: eppendorf.group/hpc-pipettes
👉 Free Pipetting eBook: eppendorf.group/hpc-pipetting...
CHECK OUR DETAILED PIPETTE TUTORIALS:
Ep. 1 - How to pipette in 5 simple steps: • How to Pipette in 5 Si...
Ep. 2 - Tips for multi-channel pipettes: • How to Fill Microplate...
Ep. 3 - Tips for electronic Pipettes: • How to Increase Pipett...
Ep. 4 - Inside your pipette: • What's Happening Insid...
Ep. 5 - Challenging liquids: • How to Pipette Challen...
Ep. 6 - Maintenance for your pipette: • How to Take Care of Yo...
Ep. 7 - The correct consumables: • How to Choose the Righ...
Ep. 8 - Tips for dispensing: • How to Properly Handle...
Thank you for watching the video & please share YOUR THOUGHTS or QUESTIONS in the comments below 👇 - Наука та технологія
The amount of professionalism shown in this video is awesome.
can't relate lol
) poppa
you are correct
idk what youtube algorithm is doing but its already far beyond human comprehension. I watched the entire thing even though i dont even own or use this stuff.
Why don't you? You should get them! You're missing out! Think of all the fluids you could pipette into vials! And then pipette them out of those vials into other vials! Tbh I reckon this should be an olympic sport. >_>
I'm kidding, I'm kidding. But it can be useful for routine testing of things. Are you a gardener? Fancy hybridizing or cloning a specific plant to improve it or make it resilient to something? NPK measurements of hydrponic solution benefit from good lab technique like this, note it all down, and then perfect your mix!
@@bashkillszombiesHaha olympic gold medal for pipetting sounds kind of nice. But yes i agree, its useful to know some day. I have actually grown some oyster mushrooms once and that was quite hard since everything had to be sterile. I have grown some plants too. Who knows maybe some day i can use this.
Damn, she handle those pipettes perfectly with no tremors
Thank you. Will be using this as a reference video for my trainees! Very clear and valuable resource. Love my eppendorf tools too!
Loved this video, extremely helpful and filled with a lot of information no one else provides in a very short video. Thank you
Thanks for this video, I need to learn about biosafety for my job. I had no idea what pipetting was. lol
The way she squeezes that pipette with professionalism is incredible especially that slow motion.
True. Its almost erotic the handling of that Pipette.
@@sebastien8415 this is about a pipette you horndog
@@aura3n you don't get my humor, right?
@@sebastien8415 :(
thanks for the explanation on pre-wetting
I really love you. You are an amazing teacher. No one has never explain it so accurately than you. Thank you so much.
Abba chaa
this showed me what to do correctly so I don't mess up the tool or experiment
Thank you for this tutorial.
Best pipetting video. Ever.
awesome
1st of all why was this recommended to me?
2nd of all I am on vacation right now, I didn't really want to have anything to do with work related things.
3rd and last point. I use the pipette frickin everyday in the lab, for years by now, I should know how to use it.
So tell me what has been going on in youtubes mind?????
So why is this in my recommendations
Same, dafuq
its interesting
if u studying or working in biomedial field it's necessary to know this
Yeah why
If you're majoring in something related to medicine, chemistry, biology, you'll understand why.
Thank you for your video.
This is work out me thanks 😊👍👍
Would you suggest completely ejecting during aspiration with an electronic eppendorf pipette as well?
Very nice explanation
Thx, Im sure now, I have done it correctly the last two years
thk's so much
My father also works in eppendorf
@eppendorf Is there any written guide for this? If there is, can you please send me the link?
her voice is so relaxing...
This video showed a good up-close demonstration 🙂
Thank you
Why is this on my recommendation?
Well, additional knowledge always wellcome
the ultamate, "thats what she said," video doesnt ex.....
Nice video
are those tips universal for this model and other mfg'rs models too ?
Eppendorf tips are mostly universal except for certain manufacturers like Rainin but matching manufacturer tips with each pipette will produce the greatest accuracy and precision.
Fabulous ☺️☺️
I use a pipette every day at work, but UA-cam recommended and here I am
Fantastic
Informative
Super madam.. 👌👌
How you stay relaxed at the second stop tho,?
Please use this thread to add your own pipetting tips too. Thank you :)
Does the depth at which a tip is sent into the "sample" effect the accuracy as small portion is stuck outside esoevialky for yge 20 ul pipettes?
This German chick speaks better English than I do.
This is so amazing
Good very good
Is there a way to accurately pipet cell suspensions? There are always residuals on the tips. Cheers!
You have to pipette in the exact same way. If there is any residual it’s probably because it’s not liquid only but some of the cells or their debris might attach to the tip.
is accurate delivery affected by altitude ? here at mile high colorado water boils less than 100 C.
I would think so, as air pressure affects accuracy
How about reverse pipetting?
Thanks akka
So much for that! I though I was going to learn how to better load weed into a bong!
Wow!
Tq
When dispensing the liquid does the pipette tip need to be against the wall of the test tube?
And must the pipette be vertical position when dispensing the liquid?
Thank you!
I think when dispensing the liquid, the pipette needs to be at an angle of between 20-40 degrees so not completely vertical
Bruh im always shaking when i put the caps on
Great 👍 mam...👏thanks for this video, can you tell me what's the difference between ml and meu l
ml = 10 ^ - 3 (milli litre)
Meu L = 10 ^ - 6 (micro liter)
Therefore, 1000 Meu L = 1ml
It doesn't show how to adjust the amount
What is the good immersing depth for good calibration?
As long as the tip is in the liquid it’s fine.
Focus on the processing of pipette tips by international brands, high-end customization, quality assurance, welcome to consult
Pipe up!!
Always wear eye protection 😎
Alexander Cook PPE 😎
0:45 "there's no need for force..."
your mileage may vary...
Is anyone else watching this because practicals have been banned due to corona?
What about wiping down the sides of the pipette with a tissue?
If the pipette tip is clean and attached from the original container and not immersed into the sample beyond the minimum necessary depth, there is no need to wipe the tip. Wiping only increases the opportunity of absorbing the sample from the tip and rendering the sample volume inaccurate.
You do not need to wipe the tip. Also with the small volumes you pipette with these at times it could greatly affect your results. But since these tips are not wiped you should pay attention to immerse your tip just enough for it to take up the liquid
Pushing to the second pressure point allows all the liquid within the tip to come out.
Normally wiping is only done with large glass pipettes and even then the tissue shouldn't touch the tip as to not suck out liquid accidentally
Nice
ok. I think the same too. Do you have this instrument in your college lab?
@@anisheshdas362 I think the equipment in our college is little small
@@rashmiranjanmohanta5693 hehe
what does sasperated mean
aspirate: basically suck up thru a tube
What about the precision rating? Whats the difference between electronic & manual pipette?
That's why you calibrate them.
It actually also differs from manufacturer to manufacturer. And depends on the range of the pipette. Reference precision scales can be found in the relevant protocols on the companies website (look up your pipette model and then the calibration protocols).
You'd still need to check your pipettes in regular intervals and send them in if your result is outside the referenced precision scale
Hi molecular bio 2 period people
I wished she would've shown both ends of the pipette when measuring. It gives room for misinterpretation if you don't show both actions at the same time.
1. What if the angle of immersion & ejection is not followed properly?
2. What causes bubbles while pipetting?
If the angle of immersion is not followed properly, there'll be inaccuracies in the amount of fluid withdrawn as well as drawing up of air bubbles especially if the fluid withdrawn is of low volume (for example, withdrawing 0.2ml from a 0.35ml sample.)
If the angle of ejection is not 20-45° and in contact with the deposit vessel walls, there could also be erroneous delivery of such sample.
Air bubbles are caused by different factors including poor immersion depth (the commonest), the type of fluid, using the wrong pipette tips, releasing the plunger too fast and some other factors that depends on the skills of the person using the micropipette.
@@BoluwajiPieters Thank you! How to tighten the tip ejector? Any suggestions please.
@@p.m.satyanarayana8505 Choose the appropriate tip. When putting on the tip, apply a little pressure on the tip to make sure it fits perfectly to prevent any spaces around the borders of the tip and the micropipette. The pressure should be enough to make the tip stay on the pipette and also enough to make the tip fall off when it's time to eject the tip using the ejector slide on the side of the pipette.
@@BoluwajiPieters The metal part that lines the micropippette gets loose sometimes. So properly using the tip can fix the problem?
@@p.m.satyanarayana8505 Then that's probably a mechanical problem. If I understand you well. You can use newer pipettes or send the said pipette for repair if the tip isn't gripping the pipette because the metal part is loose..
I'm sorry, but why exatly do you have to repeat the initial aspirations two to three times?
There's air between the liquid and the piston, so aspirating a few times lets surface molecules evaporate off the surface and saturate that air. This way you don't lose anything potentially significant to evaporation into that space. That's the explanation on ThermoFisher's site. Maybe it's most significant for very small volumes?
Didn't know what this was and was curious - saw the beautiful lady with the sensational voice and decided to say......after 3:09mins I didn't know know what this was all about. Bye for now Dr. Henke.
At the end she forgets to mention pre wetting of the pipette tip...
I just realised i stopped at ball pippetes, while scientists use fucking computer controlled pipettes.
Why no safety goggles?
Oh man I've been doing it wrong. Vertical aspirate it first
Don't over dip the pitpete tip into the liquid
🌸🌸🌸
I need PDF file can. U send me
I don’t know why I watched this entire thing
where can I buy this woman?
Don't know why this was recommended, but I'd pipe that lol
Hadir 050
wait am i the only one that actually looked for this?
This was great…the sexual tension in this video could rival the surface tension on that liquid. Entertaining and informative…excellent job.
212K people wanna learn how to pipe that’s sad
Not enough people take the time to learn this important skill.
It's not enough people until not every director, actor and writer of a sci-fi movie or show have seen it, because they show pipetting very wrong…
Intelligent women are hot!
The sexual tension is real
Lmfao
She's very professional and neat.
I'd probably let her handle my pipette any day
She's hot
el0n mus k sax tape
Thank you