It is really nice, thank you! Can you also make a tutorial of- 1) adding scale bars in these figures 2) Annotating using Inkscape 3) How to open .eps files?
In short, maybe. Many images made via scientific detectors (cameras, PMTs, etc) are set at 72 dots per inch at bizarre dimensions (e.g., 17 inches X 22 inches). So when opening in Inkscape you need to resize. What you're resizing is the DIMENSIONS, not the intrinsic pixels per inch (PPI) which is used interchangeably with dots per inch (DPI) although DPI is a reference to dots of ink when printed on a printing press (at least not while you're working on the figure). Generally the idea is to have twice as many pixels as one would need for consequent dots according to Nyquist sampling rate formulas. On a confocal, for example, the ideal sampling rate is 2.2X the number of pixels necessary to preserve spatial resolution. So, in publication, you read that images need to be 300dpi for reproduction on a printing press. What that means is you will need to change the DPI when saving the figure in Inkscape. In the newest version, you change that when saving to a PDF or EPS file format via a prompt which asks you at which DPI you wish to *rasterize* your image. Be sure you put in 300 or 400, depending on requirements of the publication. If you don't set the rasterized DPI it will be 96dpi and will look horrendous and out of focus in publication.
This is a great tutorial. But have you considered doing it with sound? It helps the viewer to follow up your steps.
Excellent piece of work.
It is really nice, thank you!
Can you also make a tutorial of-
1) adding scale bars in these figures
2) Annotating using Inkscape
3) How to open .eps files?
While resizing, will the image dpi change?
In short, maybe. Many images made via scientific detectors (cameras, PMTs, etc) are set at 72 dots per inch at bizarre dimensions (e.g., 17 inches X 22 inches). So when opening in Inkscape you need to resize. What you're resizing is the DIMENSIONS, not the intrinsic pixels per inch (PPI) which is used interchangeably with dots per inch (DPI) although DPI is a reference to dots of ink when printed on a printing press (at least not while you're working on the figure). Generally the idea is to have twice as many pixels as one would need for consequent dots according to Nyquist sampling rate formulas. On a confocal, for example, the ideal sampling rate is 2.2X the number of pixels necessary to preserve spatial resolution.
So, in publication, you read that images need to be 300dpi for reproduction on a printing press. What that means is you will need to change the DPI when saving the figure in Inkscape. In the newest version, you change that when saving to a PDF or EPS file format via a prompt which asks you at which DPI you wish to *rasterize* your image. Be sure you put in 300 or 400, depending on requirements of the publication. If you don't set the rasterized DPI it will be 96dpi and will look horrendous and out of focus in publication.