Nanopore sequencing technology

Nanopore sequencing technology - This lecture explains about the DNA sequencing process using the nanopore technology.
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[@kazmi_collection7065]

@@shomusbiologyofficial try to explain in urdu

[@ShivamDubey-rb7ed]

This is the best video on nanopore sequencing. Shomu you are incredibly amazing,.

[@ATinyGreenCell]

Thanks for always making quality bio videos!

[@irinahaidukevich1607]

Thank you so much for simple and
comprehensive explanation! You are the born teacher!

[@shomusbiologyofficial]

Thank you. Glad you liked it

[@karthikeyanbio]

PLease update the topic to Minion sequencing, now after NASA announced the first sequencing in space station using minion, I have searched in youtube but no proper explanation video about the technique except this. So please update the topic you will get more hit for this video.
and shomu. thank you many for taking effort to explain the biological topics in simplified way.

[@MukherjeeKeya]

Dear Soumu if possible please upload another video explaining basics of library prep methods, emphasizing on four main steps : end repair, DA tailing, Adapter binding and tethering ..... finding difficulties to understand. Thank a lot for your kind efforts.

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my thesis is about whole genome sequencing of a bacteria and you explain it so well, thank you so much. I will definitely mention your channel on my thesis!

[@poojaacharya2773]

The way you have explained ONT is awesome. will you please upload the tutorials for post sequencing data analysis in minion sequencing? starting from basecalling to identification of SNP?

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+kondwani Mulungu glad it helped.

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[@shomusbiologyofficial]

You're welcome. Glad to hear that you are getting benefit from the videos

[@samrudhikale1819]

You are jst amazing! I have been trying to understand nanopore seq from book...i got confused...bt thn i watched ur video..u explained in very proper way..which made me easy to understand....i am grt fann of ur teaching.!

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[@areebanaeem2162]

​@@shomusbiologyofficial sir plz make a video lecture on SOLID sequencing

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[@adilsiddiqui123]

excellent simple explanation!

[@shomusbiologyofficial]

+adilsiddiqui123 thank you. Glad you liked my lectures and

[@fatmaaybukem6557]

I am preparing a presentation about nanopore, and watching you again. I watched all your sequencing videos when i had to present another presentation last semester too :d Love your videos, keep doing ! How could you disslike the video btw??

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+yanjun dong I certainly don't know everything.... Thank you

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[@sann6688]

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[@rauldw2544]

I would like to add that the protein channels should be couple with an exonuclease which allows to unzip the dsDNA. It’s important to show that up.

[@shivanshetty8057]

The alpha teacher...❤

[@saajidmusharuf4548]

really impressive . you made it so easy . thank you so much

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You're welcome

[@saajidmusharuf4548]

@@shomusbiologyofficial 😇❣️

[@afolabioluwanifesimi1231]

Hello, thank you for your teaching. I am taking biochemistry for the first time watching this. Using the artificial method of the Silica plate, did you say we start adding "atoms' or "ions' to the pore wall? Anybody please help me understand better

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[@tophat2115]

will the protein be identified by its elemental components, say it contains, hydrogen, oxygen, potassium, nitrogen and carbon as an example. Can it identify these elements and their proportions in the sample protein?

[@ug783d]

Great, explain easy to understand nanopore, Thanks a lot,

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[@NarenAnantha]

great presentation

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Thank you

[@arshiaamjad9220]

very helpful

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[@avocadosnumber498]

Thank you! Can you explain a bit about the adaptor protein that guides the sequence to the nanopore? Is it a universal adaptor that binds to the ends of all DNA, and if so, what is its target on the DNA and how does it bind?

[@Sahil-dz5ur]

Please suggest me a book to study about NANOTECHNOLOGY and NANOPARTICLES

[@dhahihusain6463]

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[@subhodipchakraborty9140]

Nice explanation 👌

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[@ramsathish7880]

My seminar topic was nanopore sensor..i am confused in finding information plz help me

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Super recommended

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Thank you

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[@Edu-Tech952]

Sir can you please make a video on
Nanobodies as a tool for protein structure and function studies
Its really needed, want your help on this topic🙏

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After getting phd, post doc, there won't be any kick left in me like other degree holders. You don't need phd to teach well.

[@biotechexpress3416]

Shomu can you give reference of information you mentioned at around 58 seconds that the technology was underdevelopment since 1955. Please give the reference of this claim.

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shut up you fool, do not question Shomu

[@johnkoay8097]

Can the pore be blocked?

[@meftahuddin3969]

I think you are doing good, but I have some suggestions which I need to mention. What you are saying is totally different to what you are presenting. I think you should have to look the real schematic diagram of the nano-pore sequencing tools. Don't take it negatively, you are presenting it, but I want you to present it in a best and proper way. Thanks

[@shristymishra5036]

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[@simranm8475]

U and the subject are one entity

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[@siriveludharani6338]

Tq sir 🙃🙏🙏🙏🙏🙏🙏

[@shomusbiologyofficial]

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[@sananoor6230]

PLease update the topic to Minion sequencing

[@divvyasethi1572]

is it a 3rd generation sequencing technique ?

[@siddharthadas86]

no. I think it is the 4th generation. 3rd generation was single molecule approach of pacbio

[@divvyasethi1572]

thanks alot :)

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Thank you

[@sanaikram3886]

Plz sir you also describe in urdu. Because your method is very effective and we easily understand

[@shomusbiologyofficial]

Thank you

[@abantikabiswas5624]

A video on PacBio sequencing please

[@shomusbiologyofficial]

Ok

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