Alkaline Phosphatase | Poly nucleotide Kinase | use of AP and PNK in molecular biology |end labeling
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- Опубліковано 8 вер 2024
- This video describes the usage of Alkaline Phosphatase and Polynucleotide Kinase in molecular cloning and end labeling. This video would answer the following questions:
What is the function of polynucleotide kinase?
Why we use alkaline phosphatase in cloning?
What are the roles of alkaline phosphatase and DNA ligase enzymes in recombinant DNA technology?
What is the role of alkaline phosphatase in RDT?
When Labelling DNA What is the function of polynucleotide kinase?
How do you label the 5 ends of DNA?
How do you label the three ends of DNA?
What exactly does polynucleotide kinase do?
I think the representation of -PO4 and -OH group at either end (any one) is wrong (0:57 min). You need to swap the functional groups at one of the two ends to maintain the 5' to 3' orientation correct. But you corrected it afterwards at 1:10 min. The video is very useful by the way. Best wishes!
Learning from Bangladesh.
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Very nice video.. thank you
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
ha man happy teachers day thanks you giving your time to teaching and teaching good .
Thanks for wishing.....it keeps me motivated
🙏🙏
outstanding
🙏🙏🙏🙏🙏
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
Thank you so much this was super informative
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Something to comment, polynucleotide kinase use dNTP labelled in the gamma phosphate, not alpha. This last one is used by polymerase.
Can you please make a video on packaging extracts. These videos are great.
I am unsure about this topic. Could you please elaborate
@@animatedbiologywitharpan
Packaging of phage, amber mutation part and SPI selection.
Basically the red and gamm genes part. It's actually a bit confusing and it would really help if you can make a video on that because I have been referring to your videos a lot for rdt and bio- techniques.
Thank you.
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watch all my youtube videos.... you don't need to waste hours and hours in tuition classes... these short videos would give you all the necessary concepts that you need to prepare for any biology competitive exam.
debapriya das 9987997041
Sir how oH is left behind when we remove phosphate ?
Nucleotide have 3 phosphate the phasphatase removes all the three phosphates or only one phosphate ?
Which software do you use for this beautiful representation and diagram
Only PowerPoint, if you want to learn please email me at arpanparichha1994@gmail.com
1.40 , Polynucleotide kinase only acts on 5' end , then how it add phosphate in 3' end of PCR products (As you mentioned)🤔🤔🤔🤔🤔🤔🙇🏼♂️🙇🏼♂️🙇🏼♂️🙇🏼♂️
Thnku
Please please share my channel link with your friends and help me to reach big audiance
I think the PCR product does contain 5' phosphate group and does not require to be phosphorylated after PCR reaction by polynucleotide kinase. Please correct me if I am wrong.
No you are correct in your point.... the trick prople use in lab is to put phosphate group also in the 3’ end using pnk so both 3&5’ are phosphate and the vector you have to use you use alkaline phosphatase to cleave 5 prime phosphate and as a result the efficiency increase
May be I should have explained it better
@@animatedbiologywitharpan Thanks for clarification.
What is exchange reaction by polynucleotide kinase?
Can anyone explain it?