DNA ISOLATION PRACTICAL - STEP-BY-STEP GUIDE AND RESULTS!" - CBSE CLASS 12 BIOLOGY ,

1. #DNABiologyPractical
2. #CBSE12thLab
3. #DNAResearch
4. #ScienceExperiment
5. #LabLife
6. #BiologyStudents
7. #DNAIsolationProcedure
8. #BiologyClass
9. #CBSEPractical
10. #LabSkills101
1.role of methanol in this practical
Methanol is used in DNA isolation to precipitate DNA from the solution. It causes DNA molecules to come out of the solution, facilitating their separation and purification.
Methanol is added to the DNA solution to lower its solubility, causing DNA to precipitate out of the solution. This precipitation allows for easy isolation and purification of the DNA.
2.why we use cold methanol or ethanol reason
Cold temperatures increase the solubility of salts while reducing the solubility of DNA, leading to the precipitation of DNA strands. This step helps in obtaining a higher yield of pure DNA during the isolation process.
3.role of detergent in this practical
Detergent is added during DNA isolation to break down cell membranes and nuclear envelopes, releasing cellular components, including DNA, into the solution.
4.role of NaCl in this practical
NaCl is used in DNA isolation for its role in maintaining ionic strength, aiding DNA precipitation, and facilitating protein separation. It also helps stabilize DNA and ensures optimal conditions for the isolation process.

[@mamtaawana2143]

Bro your explanation is just wow..everything is crystal clear 😊 thank you so much..
BTW.. tomorrow is my practical 😅😂❤

[@vanshgaming9812]

Mine too 😂

[@user-qy6pk5ju3j]

Same😂

[@Gauravgurjar724]

Nice🎉

[@AA-eb6qs]

Everything is crystal clear ❤❤❤❤❤ thank you sir 🙏

[@AarshiDedha-gh9sn]

Thank you for your efforts sir❤❤

[@creatorpranshu]

Thank you sir ❤

[@ruchiranjan1209]

Viva ke bare me
kuch bta dijiye sirr

[@piyushsaini9678]

Kl mera practical hai ye bht kaam aayega

[@csmeena1]

Kaisa gaya

[@piyushsaini9678]

@@csmeena1 mst

[@laukikboritkar6215]

Bhaii majja aaya achhe se samja 💋

[@LetMeExplainnowbharatpanwar]

😂😂😂😂😂😂.. mja he to chiye . 😂

[@RishabhYadav-sb6wm]

Nic

[@GarimaShakya-co9zj]

Thnk uuuu bossss❤❤❤❤❤❤❤

[@GarimaShakya-co9zj]

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[@LetMeExplainnowbharatpanwar]

Are... Wa. Sun . Ke.. mja . Aa gya... 😂

[@GarimaShakya-co9zj]

@@LetMeExplainnowbharatpanwar aaaaaap kya aiiims m hoi???

[@padhlechupchap6470]

Ek baar mai hi clear ho gaya..
1 hour before my biology practical 😂❤

[@asthatiwari4733]

Everything is now crystal clear Tq so much🤌🌼✨...

[@LetMeExplainnowbharatpanwar]

Bharat sir jab sath Ho to darne ka nahin macha ke aane ka 😂..

[@asthatiwari4733]

@@LetMeExplainnowbharatpanwar yess sir Thanks a lot 🥰✨....

[@Prashant_bhai01]

❤

[@ShivPatil333]

ASC pe padte ho kya

[@asthatiwari4733]

@@ShivPatil333 nahi..

[@KNOWN40506]

❤❤

[@vy7342]

Easy is best 😊

[@vy7342]

Idea to bhut hai banana ko crush karny ka but examiner se dar lagta hai😅😅

[@JeezAdityaa]

Examiner ki

[@ritu9757]

Thankyou sir crystal clear😄😃😃😃

[@ritu9757]

@@Rishu_kumar1734 yes

[@darshangoswami6026]

😂boss 💪 thank you tomorrow is my practical exam

[@LetMeExplainnowbharatpanwar]

All the best

[@darshangoswami6026]

@@LetMeExplainnowbharatpanwar thank you boss 💪

[@shikharana9310]

Plz explain mitosis in onion root tip experiment also

[@LetMeExplainnowbharatpanwar]

All ready uploaded

[@bijayalaxmimohapatra3184]

Can we add papain extract from papaya? Is it soluble/insoluble in banana extract

[@LetMeExplainnowbharatpanwar]

Yes .

[@adityaraj6832]

kal ke liye help hogyi

[@shyamasundar8820]

Nice sir, why you can't use sodium laureal sulphate or sodium dodecyl sulphate.

[@LetMeExplainnowbharatpanwar]

Sodium lauryl sulfate (SLS) and sodium dodecyl sulfate (SDS) are detergents commonly used to disrupt cell membranes in various experiments. However, they are not ideal for DNA isolation practicals for several reasons:
1. **Protein Denaturation:** SLS and SDS are strong protein denaturants. While they effectively break down cell membranes, they also denature proteins, making it challenging to isolate intact, functional DNA.
2. **Enzyme Inhibition:** DNA isolation often involves enzymes that are sensitive to denaturation by these detergents. The use of SLS or SDS can inhibit the activity of these enzymes, affecting the overall success of the isolation process.
3. **Precipitation Issues:** SLS and SDS can interfere with DNA precipitation steps. They may remain in the solution and affect the purity of the isolated DNA.
For DNA isolation, milder detergents, such as Triton X-100, are preferred. These detergents effectively disrupt cell membranes while minimizing interference with DNA structure and enzyme activity, resulting in better-quality DNA isolation.

[@Jaishreeraam720]

Bhaiya pure world me nhi hai iss se badiya vedeo ❤❤

[@LetMeExplainnowbharatpanwar]

App sab logo ka pyar he 😊😊

[@vy7342]

Salt bhi use karte hai kya ?

[@LetMeExplainnowbharatpanwar]

Yes

[@jashwant9adilaxmi391]

Shai peg banaya sir😂

[@santanadebbarma5885]

CAN WE USE SAVLON

[@LetMeExplainnowbharatpanwar]

Yes

[@365Creator]

Kal ka practical h bhai😂

[@LetMeExplainnowbharatpanwar]

Lage raho munna bhai 😂😂😂

[@Lol-pn6st]

Hamko papaya se karwate ,crush hi na hota aache se

[@LetMeExplainnowbharatpanwar]

Are koi bhi fruits le skte ho

[@itsevilbro]

kela kha liya tha💀💀💀

[@LetMeExplainnowbharatpanwar]

😂😂😂😂😂😂 Sai kiya...