there is a mistake in this explanation you said the selective media lacking trp and leucine and plate containing trp and leu. but in diagram you have written selective media lacking trp nd leu. and plate lacking trp and leu. please correct it very confusive
My videos usually are based on multiple resources. For this video I read some research articles and books and covered basic concept. Based on my imagination gave it animated. If you are a research student, I recommend you to refer related research articles.
How do we make sure that some other transcription factor (instead of our plasmid inserted bait & prey) can't turn on the transcription of the reporter gene?
Thats an excellent question. TF binds to specific UAS sequence and not any UAS, hence highly specific in expression of reporter gene. Also, negative controls are always used.
If we place both of the proteins in one plasmid, we also need to place the AD and DBD in the same plasmid. That means the domains (AD and DBD) are already in close proximity, leading to the recruitment of transcription machinery and ultimately results in the expression of the reporter genes. We can,t study the Protein-Protein interaction by placing both the proteins in one plasmid.
best explaination of Y2H ...well written definitions and easy to understand
@Sarthak. Thanks
Thanks for saving time due to your very simple explanation. Keep it going!
thanks..
This is the best explanation of Y2H. Not a single soul on this Earth can explain it this well.
Hat's off !
Thanks a lot.
Great explanation, thank you!
Great i understand it very well. Thanks for your efforts 😊
Informative, clear my doubt.Thankyou sir
Very well documented and easy to grasp even small details! Thanx!
thanks..
best way to explain ; BIG THANK YOU
Thanks your comments acts as motivation to make more such videos..
there is a mistake in this explanation you said the selective media lacking trp and leucine and plate containing trp and leu. but in diagram you have written selective media lacking trp nd leu. and plate lacking trp and leu. please correct it very confusive
It is really great video, awesome
Good explanation, !Thank you!
Very nice explanation 🎉
very clear and concise video! namaste
Glad you enjoyed it!
love this! Thanks
Very very clear. Thanks.
Very clear. Thank you!
Awesome 🧡
Very nicely explained.
Where do you get the information from? Is it from a paper, a book? Please let me know as my research project is on this and it would be very helpful.
My videos usually are based on multiple resources. For this video I read some research articles and books and covered basic concept. Based on my imagination gave it animated. If you are a research student, I recommend you to refer related research articles.
Thanks for the explanation
Stay tuned. Thanks
You are a lifesaver
Thanks Ashley
Very helpful
How do we make sure that some other transcription factor (instead of our plasmid inserted bait & prey) can't turn on the transcription of the reporter gene?
Thats an excellent question. TF binds to specific UAS sequence and not any UAS, hence highly specific in expression of reporter gene. Also, negative controls are always used.
you could counter-select using the Reverse Yeast 2 Hybrid Screen. Look it up
Thank you is very ilustrative! I get it :D
Why can both proteins not be on the same plasmid?
If we place both of the proteins in one plasmid, we also need to place the AD and DBD in the same plasmid. That means the domains (AD and DBD) are already in close proximity, leading to the recruitment of transcription machinery and ultimately results in the expression of the reporter genes. We can,t study the Protein-Protein interaction by placing both the proteins in one plasmid.
how false positive test of yeast 2 hybrid can be eliminated
Don't understand the leu and Trp related lines.
I know its a bit confusing. Watch it twice. For more details read some book.
what is the name of the plasmids?
This is just an explantion
Thanku
Unique way of teaching thanks sir
Gud
Namaskar :)
Shomu,,,?