How to perform PCA on single-cell RNA-Seq data in three simple steps

[@mikelawrence5507]

Nice summary for reasoning behind workflow! I was looking for an example of PCA but only saw t-SNEs

[@黄梓煊-g9u]

Really great video, thanks a lot for explaining PCA so well in such a concise way

[@jattpigeonscorner9368]

Great Content. Please keep uploading

[@pariaalipour61]

Thanks for the great video. I have a question regarding scaling step; in the scaling step as you mentioned we shouldn't multiply by like a million and the number is median/total umi count. In Seurat NormalizeData function does divide the count by total cell count and multiply to 10000 scale factor. I was wondering what is the difference?

[@ilyasimutin]

Thank you, great lecture!

[@diraczhu9347]

Great video, it helps !

[@derricmorgan2282]

Nice video, thanks!

[@derricmorgan2282]

the only thing is the sound quality, I had to adjust volume and the sound is a bit rattling.

[@derricmorgan2282]

so nice that you included the links, the papers are really interesting