Thanks for the great video. I have a question regarding scaling step; in the scaling step as you mentioned we shouldn't multiply by like a million and the number is median/total umi count. In Seurat NormalizeData function does divide the count by total cell count and multiply to 10000 scale factor. I was wondering what is the difference?
Nice summary for reasoning behind workflow! I was looking for an example of PCA but only saw t-SNEs
Really great video, thanks a lot for explaining PCA so well in such a concise way
Great Content. Please keep uploading
Thanks for the great video. I have a question regarding scaling step; in the scaling step as you mentioned we shouldn't multiply by like a million and the number is median/total umi count. In Seurat NormalizeData function does divide the count by total cell count and multiply to 10000 scale factor. I was wondering what is the difference?
Thank you, great lecture!
Great video, it helps !
Nice video, thanks!
the only thing is the sound quality, I had to adjust volume and the sound is a bit rattling.
so nice that you included the links, the papers are really interesting