Yes, that's not correct. At acidic pH, basic compounds tend to undergo ionization, releasing ions and becoming more soluble in water. The specific behavior can depend on the nature of the compound and the pH conditions.
Dear sir, I have one doubt in analytical method validation. When the monograph method is used for validation in compound having more impurities and some of impurity (two or three) elutes same RT how to arrive RRF in this case further monograph mentioned RRF is 1. Please suggest the way forward 🙏
Only the master can make tough concepts to very understandable way... u r my master, bhaskar sir
Beautifully explained importants of pka vs pH and their retention in RP-HPLC thank you Sir
Excellent explanation, keep doing good work
Simply and easy explanation, thank you for sharing such awesome content.
Grat work! carry on..❤
Thank you for this, very helpful for method development. Cheers!
Valuable knowledge , thanks sir
nice information...its useful to pharma analysts
Nice and simplest explain
Could you tell me the app you use to draff, sir?
If sample contain more than 1 analyte I.e 2 or 3. How pka value and pH Mobil phase calculate??
Very nice 👌
Thanks sir
Nice explained sir
Superb Superb Superb Superb Superb Sir
Good Sir & Thank u ...
PKa of my compound is 7.6. What should be the pH of my mobile phaase?
Sir, if our compound having acidic and basic both, then how will manage the mobile phase pH.. please reply
Really good content.
Thanks Sir, it will be just opposite in normal phase column right? Please correct me if I'm wrong.
If pka is 17 , what should I have to do.
Very good please go on about troubleshooting of HPLC
Sir, if both drug pka I'd 8.71 & 12.49 so, how can I select mobile phase.
Sir what about neutal molecules?
Neutral molecule not affect ed by ph change
Nice sir..
What a wonderful presentation it is, which I have never been gone through.
how do we know the pka of new compound?
Thank u for your comment. watch my video for pka measurement.
But, acidic compounds retained in acidic pH and basic compounds retained in basic pH.
Yes, that's not correct. At acidic pH, basic compounds tend to undergo ionization, releasing ions and becoming more soluble in water. The specific behavior can depend on the nature of the compound and the pH conditions.
Sir please make video on HPLC column selecting
Excellent sir,make more videos on usp general chapters
Right...please make video sir
Sir, what impact will be there on LC if Mobile phase's pka and Compound's Pka is same.?
PEAK BROADENING
Compound remains in which state while retaining on column? Also plz make a vedio on behaviour of zwitterion in chromatography. How does it behave?
Sir how to know compound acidc or base
Thank you sir
Why not Upload new Videos Sir
Thank you❤
Why To Not Updated Any Videos
We will have few very soon...
I Want column chemistry
Dear sir,
I have one doubt in analytical method validation.
When the monograph method is used for validation in compound having more impurities and some of impurity (two or three) elutes same RT how to arrive RRF in this case further monograph mentioned RRF is 1.
Please suggest the way forward 🙏