[LIVE] Learn Genetic Engineering - Part 1: How does it work?
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- Опубліковано 20 чер 2024
- Have you ever wanted to learn genetic engineering? Well today is your lucky day. This series will get into the nitty gritty of how it works, starting from the basics of DNA design, to assembly strategies, and even genetic logic. This episode is an overview of the basics, how one goes about planning and preparing for an experiment, and a lot of resources to help you learn.
Donations: streamlabs.com/thethoughtempo...
Past genetic engineering streams/videos:
• [LIVE] Using HIV + Ma...
• I Grew Real Spider Sil...
Sebastian's work:
atinygreencell.com/
Books:
Essential Cell Biology - Alberts, et al
Biobuilder - Natalie Kuldell et al
Synthetic Biology: A Primer - Baldwin et al
Concepts of Genetics - Klug et al.
Other channels:
/ @ibiology
/ @crashcourse
Tools:
benchling.com/
www.snapgene.com/
Other resources:
www.ncbi.nlm.nih.gov/
parts.igem.org/Catalog
www.jove.com/
www.researchgate.net/
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Become a member: / @thethoughtemporium
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Website: thethoughtemporium.com/ - Наука та технологія
POV you put on a Thought Emporium video to try and sleep but now you're sat at your desk taking a genetic engineering lecture and writing notes at 1am
Me rn
literally me but 5 am
POV YOU USE POV WRONG
5:47 now
@@alexlewis4331 pov who cares
I'm currently at the end of my 2nd year Bachelor's on Biotechnology and Genetic engineering. You were actually my main idol and inspiration.
My total inspirations are 1. You, 2. Jurassic Park & 3. An overgrown geko on my ceiling :)
"Jurassic Park" ...
lol are you saying you genetically modified a pet geko to be larger than normal?
Also what college/university are you studying at? Because its extremely rare to find such a degree program anywhere.
@@plagueainsworthdenny375 What's that suppose to mean?
@@paulrichardson2554 the general stigma around the shows/movie's
Please continue doing these so we can have these resources to learn outside of academia! 🙏
Yes and tell us what you and people like you need so we coud have nice things like this.
@@Beidoss I was thinking about what creators like him need so he coud make more videos to educate us,but thanks for the information.
just an observation : academia and self learning have strengths and weaknesses. the ideal is to pursue both. This is hard, but necessary.
@@hrig problem lies in academias modern corruption most people like myself who are capable of educating to that level get turned off of academia because it's not worthwhile they charge exorbitant amounts to then sell you textbooks and say read the textbooks may as well get the textbooks and self study and find a way to fill the gaps thankfully people love talking about their field of study so it's easy to get answers
@@Jazzafritschthe first part of your response had me worried. Thank you for not going where I initially thought you were headed
Your contribution to the educationally aspiring community is valued and appreciated.
I have done many literature searches in my degrees for classes, and I cannot tell you how many papers have way more convoluted sentences that could be shortened to 2-4 words! They don't need peer review! They need editors!
I was able to help streamline my husband's Master's thesis and further research papers such that my husband's professor exclaimed I knew a heat transfer concept! I didn't and don't, but I know how my language works.
I red-marked my husband's first paper throughout. After that, the only page that got more than one or 2 corrections was the first page. (which I totally ripped apart!) It's like all he needed was to rev up a little, and he got better at writing after that.
I been in uni for 4 years now (biomedical engineer) and kind of lost the feeling that I was actually interested in the topics being explained in my courses, but now even after just half of the video I feel like much of my motivation is back, thank you so much!!
Hmm what do you think why is that ?
could you tell me more about your major please? ive done some research regarding BME and i like what ive read, but i dont know if im ready to choose it next year
Please, please continue this work. I'm currently a junior working toward my BS and in independent research utilizing the CRISPR-Cas9 system to knockout cisd2 gene using zebrafish as a model. I love having an external learning extension outside of the lab and academia! Thank you for this!
how much did it cost you to do it?
This video took me through years of my life , from the hard studies to the frustration.
I actually started my work from spider web effects on neurons so It was wonderful to see your work.
I have been extremely sick for years so I miss my work , everything you explained just flashed before my eyes , from struggling with DNA , designing the primer right and so much more . can't belive electrophoresis is still talked about !! It was ancient even when i was at school !
I enjoyed your video a lot , thank you from iran
Electrophoresis is still a good tool to get DNA straight from the source instead of manufacturing, and it's cheaper too!
@@lollsazz yeah it’s standard in most (all) molecular biology labs haha. For dna or proteins
@@lollsazz yeah ! Because it was cheap they taught it to us ! Never got to use it at work though , always needed more accuracy or had better tools to use
@@tainicon4639 always had it in the lab , I had fun playing with it ! All I needed was spectrophotometer , got my answers easier and better , electrophoresis never even came up for me
@@kgh8158 I’ve used it for isolating specific parts of a plasmid (throw in two restriction enzymes and get two pieces DNA with different lengths…). And just basic genotyping. It’s cheaper than running q pcr to figure out if the mouse has the floxed allele or not haha.
Also… for proteins… western boots are still very common haha (that’s electrophoresis through acrylamide instead of agarose).
I would like to see a lot more of step-4 Design the DNA.
there is a 1 hour benchling tutorial on youtube about this. What im more interested in is an explanation as to how the DNA is actually synthesized.
⁰
@@justanotherguyful Can I get a link?
@@ChrisjayH1no
It is so exciting to be in an age where you can get such good education online! Please continue doing it, i really learn so much from it! I am just starting and have an idea in my mind, but the only thing i need to know is that it is possible, and that is for sure the case. Now it's my turn to make it reality and your videos literally help so much :) And they were the reason i got into this and had my idea in the first place, so thank you i guess, have a great day, and a great future career! You are helping so much people :)
Literally the only thing keeping me alive. Lost my will to live a long time ago but after I discovered MIT OCW and the quality and quantity of UA-cam education went up, I've been cramming new things into my head everyday. Very exciting.
@@ImNotQualifiedToSayThisBut i love your name
now i love it even more.
@@ImNotQualifiedToSayThisBut the moon is exactly the same size as jupiter.
haha this is fun!
@I'm not qualified to say this, but lmao your name ! 🤣
Hi, lurker here, I really appreciate and enjoy your videos. I got really excited when I saw your shop/lab tour, I’m a machinist and your videos and livestreams make for great podcasts at work, I’m so fascinated with everything you’re up to even if I don’t always comprehend everything you’re doing! Excited for more livestreams!
As a Molecular Biology and Genetics major, your videos continue to inspire me. Keep up the good work, and thanks for sharing your experiences!
"And now the engineering is going into making better insulin"
Some would say that it is "better" in the sense that every time they make some minor change to the formula, it can be patented so that they can get all the doctors to prescribe it and make millions of dollars instead of giving people the insulin that literally anyone could make cheaply because it's not patent-encumbered.
on point. a hormone cannot be “better”.
@@chri-k Insulin IS a hormone...
@@lollsazz …
No, better as in faster acting or with fewer side effects. You can get old school insulin very cheaply. The new expensive stuff that is better is actually better, and safer.
@@relativisticvel then it is no longer insulin
Your channel was one of the factors that made be decide to pursue a degree in biomedical engineering
Thank you this will help me in singlehandedly wiping out mosquitos
before you're asking, I don't care what the environmental consequences are
Perhaps you SHOULD care what the environmental consequences are? If it ends up killing more people long-term, you haven't done a good thing.
@@Erhannis I would gladly sacrifice a billion people if it means no more mosquitos
I don't care about morality, I just want them gone , no matter the cost
@gaming prof Not sure why I didn't realize that, thanks
@@Erhannismosquitoes are estimated to have killed HALF off all humans that've ever existed. i'm sure it won't end up killing more people
1:53:26 I mean, I feel like failures are important, too. Doing videos on those could help teach things to avoid, or even how to recognize and handle failure.
Edit: somebody already commented on this, and in light of the comments you made later in the stream, I'll modify my statement as follows: failure should be documented *somewhere*, at least. Otherwise you're constructing a fairy tale land where everything works, and on top of that, anyone who wants to try something you already failed at doesn't get the benefit of the things you already tried.
One of the best ways to combat peer review flaws is to make it open source. By allowing anyone to get their hands on the research, people who not only are interested in that specific paper and actually want to repeat the resuts and add to them will relaease their own public papers that either agree, disagree or even add to the paper as whole
Theres probably decent reasons why they don’t
@@ludvig3242 yeah... money talks
@@ludvig3242completely indecent reasons
Thanks man! Love how clear you explain everything and the reasons behind everything. Fully able to comprehend it.
I’m only about an hour in…this is really good! I’m studying biology and work as a tech in a related field (nucleic acid medicine research). I’ve been working with CRISPR and related technologies for the past 1.5 years: your points on how complex it is and how expensive it is are so important. I spend a massive amount of time researching genomes, technology, and plasmid maps and designing everything in silico before even starting to think about attempting it in the wet lab. Even so they don’t always work; frequently not the first time and sometimes it just won’t work. Despite the frustrations I don’t want to work on anything different.
I've never emphasized more with a man in my life
ALSO THANK YOU A thorough GENETIC ENGINEERING VIDEO THAT ENTERTAING COMPREHENSIVE HAS GOOD AUDIO QUALITY AND IS JUST REALLY GOOD
Empathized*?
Man, you're so inspiring, I wonder how well it translates being a programmer into this field
it doesn't really translate at all. It's not the same skill set. It's closer to staring into the eye of an eldritch god and deciphering what it wants, than human code which is just math with extra steps.
There's a large and growing demand for scientific programmers in biotech. If you can educate yourself on some basic biology and get some bioinformatics under your belt I'm sure you could break into the field
@@andreasguerrero3865 Yea absolutely, most programs will eat you up if you say that you are a programmer, especially with genetics work. I'm learning bioinformatics now and its all code, people who have previous experience with it are in quite hot demand in a lot of labs now
@@Luminarada80 Which labs are hiring?
Wow, okay, I'm only at 36:45 right now, but I feel I have to chime-in now. I was mildly blown-away by how you were able to (temporarily) cure your own lactose intolerance.
I'm more of a physics and comp-sci kinda guy, with only a casual interest in bio, but protein chains are one of those areas of subject-matter overlap where I think I could actually sink my teeth into it (figuratively, of-course).
So I'm a bit floored by how you were able to get a virus to splice-in the gene for that protein, at-least in a limited manner, to the cells of your own stomach lining.
oh yeah, in the back of my mind was the risk factor, which you just started to talk about now...
honestly these streams and videos are at least 100x more interesting to me than any of my college classes. I find learning about these things absolutely *fascinating*, even though I know I'll likely never apply the knowledge ever
I really appreciate your videos! So interesting and they motivate me throughout the boring phases of university :) Actually holding a presentation about your projects!!
This is a great way to get into genetic engineering. Without over whelming your self.
I can vouch for the fact that there is virtually no regulation in the US. I used to work for the Salk Institute and they'd send me home with millions of transgenic seeds to stratify in my home fridge. My roommates would be like, "What are all these little tubes next to our food?"
Hi Justin, the three proteins you are talking about on @47:20 is the RUBY gene (pCaMV 35S RUBY). I am using this for my thesis project as an visual marker in Tobacco and Potato! Thanks for sharing and love your work!
Please continue with this stuff. You got me to learn and got interest in the bio field
Adam Mastroianni has great pieces on his substack about peer review and scientific writing. I particularly enjoy his description of most papers these days as being "written like they hate you"
Awesome!, can't wait to start growing my tomacco plants
Sounds dangerous, planning to smoke the tomatine or eat the nicotine?
What about other glycoalkaloid toxins?
@@petevenuti7355 To eat the nicotine of course ua-cam.com/video/Xx1ztJROpyU/v-deo.html
scientists have already done this, buy the tomaccos contained way too much nicotine
This should be required knowledge for legislative bodies. Thank you for putting it out there!
Just met my lab mentor and found I'm going to be researching CRISPR CAS9 with Deinococcus, this video is now my life.
You just got a Brazilian biomedical eng. follower.... thank you very mush for the video hope to see much more please.
I love your channel so much, now I pursuing biotechnology. I hope you keep doing this kind of videos. You kindda my favourite youtuber
I AM watching FROM ANGOLA (AFRICA)
so happy to have you back, science youtube woke up a little bit better for it this morning!
Thanks for the book list in the description looking forward to checking this out later
Thanks for putting all this together! I'm looking at local programs for biotechnology right now.
Stream starts at 0:33
People that "don't like GMO's" are 100% of the time referring to crops who's seed has been genetically modified to contain insecticides like roundup (and they don't deserve a capital "r").
I'd like to think that most of us understand that everything is genetically modified and as long as there isn't insecticide built into the food we eat, it's all good.
This is the exact channel I've been searching for
Nice to see you here again, it's a shame I couldn't see your stream live.
really great to hear that you're working as hard as ever on the spider silk 👍👍👍
Thank you for this video. I want to get into a science field and even as just general starting advice, this is good
Did you ever make a book and did you complete the series? You might literally be the only person on UA-cam ai can find making this content
Phenomenal presentation, thank you so much!!
Thanks for making this one of the most interesting corners of the internet
AAAAAHHH FINALLY IVE BEEN WAITING FOR A VIDEO LIKE THIS
This feels like a lecture.time to start taking notes lol
Thank you for posting your resources!
I really appreciate this series. Good introduction. Thank you, mad mycologist
In 2019, glyphosate (not glyphosphate) was shown by researchers at UW to significantly increase risk of non-Hodgkin's lymphoma. RoundUp has wreaked havoc in agricultural communities. Genetic engineering absolutely is a beautiful and necessary part of being human, but that doesn't mean it can't be turned to harm like any other tool we've used carelessly in the name of profit.
please don't stop making these 🙏🏿
love this video sm! ty for all this info!!!
We def do radiate plants to suppress or find a new locus at my job. Also the problem i see with bioluminescent plants is it disrupting certain insects or increasing predation. Otherwise they may be cool as urban or house plants
Im also a horticulture major in plant breeding and genetics. i love anything biology, just enjoy the plant focus. This video helps a ton as my courses haven't had a huge focus in the science of it outside of gen bio/chem
28:00 i feel like glowing plants would create a light pollution problem
Plant mod idea you may find interesting: replace the legumin gene in beans (or helianthinin in sunflower seed) with spider silk proteins. This could provide a method of simple to farm bulk protein growth? The trick, assuming you can get a viable organism that actuallt expresses correctly, would be processing the raw product into useable silk. I think that it could probably be done chemically in bulk.
lol I wished I had this video prior to my transition from academia into research, so many wasted hours lost googling/reading (to avoid seeking help from my PI). Great video
Really enjoying the series, only realized it was a thing by catching the tail end of the 3rd one live. For the failed experiments, a short with a "This didn't work, these are the reasons why that could be and what new trials I'll be running to test which is true" could be very useful without being a full video for something you feel is incomplete. After the first few, you'd only need to make one whenever a problem is presented or you have a novel approach to try. Discards can be useful for other purposes after all
Thanks for making this video.
Wow just in time, I am iniciating a project in my university as a graduation srudent in Agro Eng this handbook (or livebook should I say) is gonna be very helpful
Thx for book recomendation and some thoughts I intend to work at Bio-Eng crops
Needs to be a podcast I can listen to at work! Get that revenue.
I'm so glad that someone said that about GMO from an academic position. I'm referring this video to at least 15 people right now
i'm going into my senior year of my bio undergrad degree this year and hope to go to medical school for surgery (i would specifically like to specialize in gender affirming surgeries) ultimately, but i had such a great genetics professor that i've always been so interested in learning way more about it. seeing you do cool ass shit like curing your lactose intolerance with genetic engineering makes me hope that someday i can get very wealthy off of my career so that i can pursue research projects in an at-home lab as a hobby. you are currently the sole reason i'm reading research papers from professors in the microbiology department at my uni so that i can try and research under someone who specializes in genetically engineering bacteria (because i LOVEEE those little guys. they're so neat) i literally love your channel, you've just increased my already huge love of science and made me want to broaden my interests in scientific topics even more. you are such an inspiration to me, please never ever stop doing what you're doing because it is fucking awesome dude
It might be controversial, but gender affirming surgery involving genetics might be weirdly cool. It would be hard as most of it is hormones related instead of genetics, but finding a safe way for naturally t/estro production might be cool
@@megasocky i totally agree, it would be so dope (and a lot more convenient for me tbh) if we could somehow genetically engineer our bodies to naturally produce t/estro omg
I will genetically modify my garden flowers to die faster and no one can stop me!
I will genetically modify my houseplants to produce thc so the feds can’t arrest me.
@@Crozzzbonez0 I can think of some plants that already produce THC.
And they're perfectly legal already!
At least, where I'm from...
@@3OHT. lol, i live in America and it’s mostly legal here. I was more thinking like cartel shit and shipping it overseas to illegal countries inconspicuously under the guise of a plant business or something.
Although, i will add specifically for the Feds: _the contents and statements made in this comment are not a confession but are specifically crafted as comedy. They are not to be taken seriously and are in no way indicative of me or anyone related to me committing a crime. Any incidents (legal or not) before, during or after this message was made are purely coincidental and are in no way related to me._
What do you think about Michael Levin's bioelectrics work? if you've seen it that is.
specifically Id like you to comment on the 30 second response of the Xenopus' reciprocal leg when the other was cut off.
Hi.
Thank you so much for your content
I NEED the next stream, *NOW* !
Please continue, making these videos they are both fascinating and informative, many people within a similar or exact field would benefit from your efforts.
Im pretty impressed of the professionalisme and the way you did it all and saying that u are not a professional chemist ok but you defently do the work like a prof from my amateur view here😂
Thank you 😊
So much for doing this
Love your work
Cannot wait for the next part!
But could you please next time turn up your mic?
Reminder to put Sebastian's links in the description
I'm interested in an explanation as to how the DNA is actually synthesized. I understand you cannot make it yourself at home and have to hire big commercial labs to do it for you, but how do THEY do it then? What machines are they using? How does the process actually work step by step? is it impossible to do it at home or is it just economically a bad decision?
And to clarify if I am being misunderstood, I am not talking about the act of designing the DNA on the computer online and then ordering it online to be delivered already made on your doorstep. I am talking about actually MAKING the designed DNA into a physical reality. How is this assembled/made/synthesized?
2:09:00 you can 3d print mask holders that make it so N95 material goes further... But we're way past needing them.
Im a german biotech Student and im watching this at 4 am at the 2nd day if christmas after having a panic attack let's go
Missed the original stream and finally got to seeing the VOD. I really underestimated how educational this video was going to be. On the topic of not showing your failures, I understand your point of it being more effort than it's worth + people probably wont view them, but what about something like an academic equivalent of a "blooper reel?" You may not know what's going wrong, but someone else might. Also if it's truly a dead end project, it would be helpful throwing up an ontological "here be dragons" poster so people who just happen to be replicating it independently are aware of issues.
Can you please make a video explaining in detail the risks you talk about at around about the 37 minute mark? It sounds like an interesting topic
1:18:40 It’s kinda funny listening to this at work while reviewing some HPLC chromatography while he describes HPLC chromatography. My only addition would be that the way you isolate the chemical you are looking for requires not only choosing the right column but also the mobile phase, the liquid you mix with your sample to help pull it through the column. Other things that will influence the separation are things like flow rate and temperature. And there’s an entire second step of picking what kind of detection system you want to use, for example the same sample will look completely different if you analyze it at 200nm vs 300nm. But obviously that was more detail than this brief overview was being intended to give.
UA-cam randomly brought me here. Very enjoyable content 😊
Holy sht. This is free, I cant believe it
I get sich trash in my feed, and im subbed to some sick ass channels. Glad I checked in. Good shit.
I understand what you said about not posting about unfinished/ failed experiments, however I also think a lot of your viewers would still be interested you theorizing on what went wrong. Perhaps you could condense multiple experiments experiencing issues
Please continue this series. It is the only way most of us can learn Genetic Engineering since most schools dont teach it, or atleast dont teach it without wasting your time with 3 and a half years of unnecessary information bloat.
Hey, I'm curious about how plausible it would be to create a giant mushroom the size of a tree that glows in the dark. Now based on how mushrooms work it might not be possible, but would it be possible to make the fungus into a plant and put more plant like elements into it? This is purely out of curiosity, as i find the idea of a fairy like forest with giant mushrooms entertaining.
I'd say VERY complex to create a tree-sized mushroom, as tens or even hundreds of genes must be correctly modified
Please do it i’m rooting for you
@@lollsazzYeah, I'd say it's decades from now, just the sole difference between plant and fungi tissues and their development would take years to decipher, just the difference in the main building material poses a significant challenge
My Boilogy teachers professor was involved with the golden rice!
i just woke up and this was finished
Thank you so much for this
you should make a discord server for budding biologists! id love to meet people in the community
My man, chugging straight vodka like a boss while revealing the secrets of organic life.
It appears that AI would be extremely helpful in genetic engineering.
Thanks for this ya big stud
46:00
yeah this is me, failures and averages and than suddenly success and ecstasy
Id love to see a long form like this of if any of the halo spartan gene mods are plausible. Not like the physical but the chems for gene modding
What I want to find out is if there's the same Methylation pattern for the same ligands (antagonist) if it's possible to undo the precise genetic silencing that an antipsychotic does apparently permanently, and if restoring the genes some being house keeping genes, then could I restore lost phenotype by turning the same genes back on to restore my body to it's before antipsychotic permanence and cure the tardive dyskinesia? Could in theory one sequence the methylation of any antagonist to use CRISPRon to turn the genes back on and in part reverse the drugs life long permanent silencing, and restore the variety of missing protein functions?
How possible do you think it would be to alter an existing organism to have more fast twitch muscle fibers or change the vector of muscle fibers? (ie from the direction human fibers typically run to that of a sloth)
Why would you want sloth muscles?
so, you mentioned that 100ml of his-tag purification resin might go for $1,000. i looked up its composition and it looks like it could be made cheaply in bulk with a bit of chemistry
i love u dude ... keep the party going... more content
You should increase the gain on your mic your volume is really low!