I have been watching a lectures for a few weeks now, i realize that he is actually a but of a genius. Maybe we don't recognize all of them in society and some of them could be teachers like him, but he seems to be a person with multipotentiality. Intelligence, clarity, diverse subjects, hats off to this great man!
Most understandable lectures that I have found in my life, so clear explanation, I go through most of the times with the lectures what i need in my exams,, best wishes for him always
I am finding myself SMILING AT DNA TECHNOLOGY because you are explaining it so beautifully, reiterating things so we mortals can keep up and tying concepts together from film to film. Awesome. You are the go-to channel for my UK A level science teaching prep. Just made a bit of donation.
you are a legend!!!!!!!! I got an exam tomorrow and wasn't too sure of the reason cDNA was created and how it was created, keep up the great work!!!! You will achieve great heights my friend!!!
Awesome video! Thank you for making it. This helped me tremendously to prepare for my national exam. I'm not the type that can just read about a process and grasp it; I have to see it or perform it to truly understand it. Thanks again!
Damn, it was all that easy. That guy from 8 yrs ago, just destroyed my instructors 1 term period teaching skills in 12 minutes. Respect man thanks a lot.
No, DNA is not single strand. What we do first is to create the ssDNA complementary to the ssRNA. We now have to destroy the ssRNA to build the complementary in DNA. That is why we use high pH to eliminate RNA and only obtain ssDNA. Then with DNA polymerase the second strand of DNA is created. Now we have dsDNA. Nothing of this means that DNA is simgle stranded, only when we need it to be in the process using heat.
Excellent teaching skills!!! You make molecular biology to look very simple and understandable. Great job which arises from a good knowledge. Thank you.
+QuentinWolffMusic no it won't be a problem because the translation of the mRNA formed from this cDNA would have a start and stop codon flanking the gene of interest, so any no. of dNTPs before the start codon won't alter the final protein. I hope ths,helped
You have some excellent lectures! Could you do one on YAC's and BAC's. If you have already I haven't been able to locate it. Thanks for you teaching methods!
How can we know that the poly T tail won't bind to the poly A tail of another mRNA molecule rather than the mRNA molecule of interest since all the mRNA molecules have a poly A tail
You don't. Poly T-tails will be used for non-biased cDNA library generation. If you want to make gene specific cDNA only, you will need a 3' reverse primer that is sequence specific.
Why do we need to synthesise poly c tail dna primer?? We can use poly a tail dna primer it is complementary to the cDNA.. And we don't need to add terminal transferase
Maybe already have long time this video but I have a question, and I hope someone can answer me. There's no technology to create the protein using our eucaryotic mRNA and to avoid the other steps to get a protein (eucaryotic)? I mean, if we can extract the mature transcript (modified mRNA -3' poli A tail, 5' cap and spliced exons) from a eucaryotic cell, why we need to form, make or any word you want to use, cDNA and then use procaryotic cell?, unless there's no technology to use directly the mature mRNA. Thanks
because retroviruses can insert specific DNA fragments into the host original DNA, this means everytime the host cell reproduces it will have this new modified DNA which can be translated in the new RNA , if you directly insert mRNA it will only be used once because mrna degrades over time
Hi. I want to sintetize de Oligo dt(12-18) and Random Primers (d6)n and save some money. How do I have to configure de requirement (order) for the primer syntesis lab? I know that the 5' of the oligo dt must be modified wiht a PO3, but that's all I know lol... Please, help me
Your video has a mistake. You said that for the eukaryotic mRNA, the exons must be spliced out but it's the introns that are spliced out during the post-transcriptional modification to produce the processed mRNA. time 2:30
That's not a hard-fast rule. mRNA can undergo "Alternative Splicing" where exons are either spliced out or left in the mRNA. It is based on the splice site that the spliceosome decides to function at.
since the revierse transcirbatse need a primier to start doing its jop whats if we inserted a normal dna polymerase instead of the RT wouldnt it do excatly the same process of making complementry dna to the mrna ?
I don't think DNA polymerase will do its job on a single-stranded mRNA, as it cannot use the mRNA strand as some sort of 'template strand' (mRNA strands contain uracil instead of thymine). So that is why reverse transcriptase is used
This man is an absolute saint -- never seen anyone else be able to explain such complex topics so eloquently -- a godsend for MCAT studying!
I have been watching a lectures for a few weeks now, i realize that he is actually a but of a genius. Maybe we don't recognize all of them in society and some of them could be teachers like him, but he seems to be a person with multipotentiality. Intelligence, clarity, diverse subjects, hats off to this great man!
No "um" "uh"s man. Flows soooo nicely! Thank you!
he's very professional
The best lecture I've seen on this topic. Whenever I forget certain basics, I rewatch this tutorial to refresh my knowledge.
Most understandable lectures that I have found in my life, so clear explanation, I go through most of the times with the lectures what i need in my exams,, best wishes for him always
You sir are an excellent teacher.
I am finding myself SMILING AT DNA TECHNOLOGY because you are explaining it so beautifully, reiterating things so we mortals can keep up and tying concepts together from film to film. Awesome. You are the go-to channel for my UK A level science teaching prep. Just made a bit of donation.
I love this channel. You've pulled me through so many courses. Thank you
This guy killed it. Amazing talent in presentation and clarity of information. Thank you so much.
you are a legend!!!!!!!! I got an exam tomorrow and wasn't too sure of the reason cDNA was created and how it was created, keep up the great work!!!! You will achieve great heights my friend!!!
My already tremendous respect has increased ten fold for you after this video
Best video EVER!!! You are dedicated to teaching this concept and it shows, I wish you great wealth from your efforts.
Thank you thank you thank you! I've been looking for a bit information all over the web and this video contained it. Great lecture. Subscribed.
I'm not the first one who says this: You are awesome! I admire your career, thank you for doing this!
You are one of UA-cam's best. Your work is excellent.
you are an amazing teacher...thank you for explaining everything in such a clear and easy manner.
Awesome video! Thank you for making it. This helped me tremendously to prepare for my national exam. I'm not the type that can just read about a process and grasp it; I have to see it or perform it to truly understand it. Thanks again!
I swear this man is the savior of my GPA
Its really a continuous and clear effort in this filed, I do appreciate your hard work and best, simple explanations. Many thanks
explain such a complex set of procedures in a very clear logic. thanks for saving my life in the bio exam :)
So clear and succinct. THANK YOU! BLESS YOU!!!
Thank you for existing Sir, this helped a lot
thanks for your assistance which made my biology life easier as your lectures are easy to understand and straight forward thank you!!!
this is amazing and simple to understand ! thank you !
Amazing! Thank you for doing lectures in youtube!
the greates mentor i ever seen ...hope you continue making those videos
Best teacher I ever had
Thank you Sir ! You open my mind.
Damn, it was all that easy. That guy from 8 yrs ago, just destroyed my instructors 1 term period teaching skills in 12 minutes. Respect man thanks a lot.
Damn it was really 8 years ago?
@@AKLECTURES time is passing mister, you did absolutely well. We are-mbg students- still watching your videos. Thank you
Fantastic Overview of this process.
Damian Clark thank Damian! :)
AK LECTURES
Tools of biotechnology
Beautiful illustration AK
Thank you for all your videos.
you have a big SHalom from Israel. :)
Great job explaining this! couldn't be any clearer!
No, DNA is not single strand. What we do first is to create the ssDNA complementary to the ssRNA. We now have to destroy the ssRNA to build the complementary in DNA. That is why we use high pH to eliminate RNA and only obtain ssDNA. Then with DNA polymerase the second strand of DNA is created. Now we have dsDNA. Nothing of this means that DNA is simgle stranded, only when we need it to be in the process using heat.
Excellent teaching skills!!! You make molecular biology to look very simple and understandable. Great job which arises from a good knowledge. Thank you.
Excellent indeed, excellent in spreading wrong information.
I just find this channel, it's amazing!
But I have a question: The gene is different at the end, there is a new codon (CCC/GGG) which can produce another protein, isn't it a problem ?
+QuentinWolffMusic no it won't be a problem because the translation of the mRNA formed from this cDNA would have a start and stop codon flanking the gene of interest, so any no. of dNTPs before the start codon won't alter the final protein. I hope ths,helped
a very usefull subscription after a long time...
Thank you, sir... You are a life saver!
6:08 A poly-C tail should be a poly-T tail.
amazing video, perfectly explained
You have some excellent lectures! Could you do one on YAC's and BAC's. If you have already I haven't been able to locate it. Thanks for you teaching methods!
Man, you will go to heaven!
Thanks for the video, it is very clear and easy to understand, help me a lot.
really excellent teaching. Thank you very much
everything is just perfect ... awesomeee
Thanks man, great video!
thank you so much. with your clear explanation i start to love molecular biology. ;)
Thanks, sir. You are awesome.
Very easy to understand!
So helpful video, thank you so much
You are all rounder
Thank you soo much! you are genius :) So helpful
Great sir dashing teaching methodology sir god bless u
Fantastic video!
Great video, thank you :)
Thanks sir.......you are genius
Excellent !
Thank you so Much
Very useful... thank youuu !
brilliant ! thanks a ton :)
Gayathri Jaikumar you're welcome!
THANK YOU SO MUCH
thank you!
thank you so much!
Thank you
very great thank you.
thank you
well explained
i appreciate you!
Wow! Genius!
Awesome! It helped me a lot.. thanks =)
Very nc lecture sir😊😊😊...thank u
Thank you so much :)
dude thank you
Hii ! I'm from India...love your lecture...make more videos on life science.
OMG Thanks a lot!!
in step number 6 the 5prime end consists a hydroxyl group,do a 5prime end consist hydroxyl group or phosphate group?
how are sticky ends attached to the two newly generated cDNA?
Very good 👍
Silly question, do humans have reverse transcriptase or is that only found in certain viruses
A question, E. Coli can produce RNAm eukariotic? What happen with the rbs of the eukariotics cells? In your video we watch that.
thank u sir
Excellent video!
Nahu Dimitri Thanks! :)
How can we know that the poly T tail won't bind to the poly A tail of another mRNA molecule rather than the mRNA molecule of interest since all the mRNA molecules have a poly A tail
You don't. Poly T-tails will be used for non-biased cDNA library generation. If you want to make gene specific cDNA only, you will need a 3' reverse primer that is sequence specific.
We can also use RNAse H to degarde that mrna?
Why do we need to synthesise poly c tail dna primer??
We can use poly a tail dna primer it is complementary to the cDNA.. And we don't need to add terminal transferase
Maybe already have long time this video but I have a question, and I hope someone can answer me. There's no technology to create the protein using our eucaryotic mRNA and to avoid the other steps to get a protein (eucaryotic)? I mean, if we can extract the mature transcript (modified mRNA -3' poli A tail, 5' cap and spliced exons) from a eucaryotic cell, why we need to form, make or any word you want to use, cDNA and then use procaryotic cell?, unless there's no technology to use directly the mature mRNA. Thanks
because retroviruses can insert specific DNA fragments into the host original DNA, this means everytime the host cell reproduces it will have this new modified DNA which can be translated in the new RNA , if you directly insert mRNA it will only be used once because mrna degrades over time
exons that must be spliced out? I thought introns, as you said in the beginning? :/
he just said it the other way. but he puts the concepts so clear that no one notices it
Awesome
Hi. I want to sintetize de Oligo dt(12-18) and Random Primers (d6)n and save some money. How do I have to configure de requirement (order) for the primer syntesis lab? I know that the 5' of the oligo dt must be modified wiht a PO3, but that's all I know lol... Please, help me
Your video has a mistake. You said that for the eukaryotic mRNA, the exons must be spliced out but it's the introns that are spliced out during the post-transcriptional modification to produce the processed mRNA. time 2:30
That's not a hard-fast rule. mRNA can undergo "Alternative Splicing" where exons are either spliced out or left in the mRNA. It is based on the splice site that the spliceosome decides to function at.
There is an error at 2:25 ( you said "exons that must be spliced out", when it is Introns )
great vid!
Artur livshits thanks! :)
DNA polymerase isn't needed to finish synthesizing the ds-cDNA molecule. RTase has DNA polymerase activity.
since the revierse transcirbatse need a primier to start doing its jop whats if we inserted a normal dna polymerase instead of the RT wouldnt it do excatly the same process of making complementry dna to the mrna ?
I don't think DNA polymerase will do its job on a single-stranded mRNA, as it cannot use the mRNA strand as some sort of 'template strand' (mRNA strands contain uracil instead of thymine). So that is why reverse transcriptase is used
Upload a video explaining adapters and linkers please
Excelent video, but shouldn't the 5' ends of the molecules have a phosphate group instead of a hydroxyl group?
i was wondering the same thing
sir u r the best....ur teaching method is amazing...so easy to understand
Thank you so much.
And RACE PCR please!!! :)
You are a medical hero! Thanks a lot!
This is graet😎
Only partially digest RNA with RNAse H, not the entire RNA