Susan Taylor (UCSD) Part 1: Protein Phosphorylation in Biology

www.ibiology.or...
In this lecture, I have given an overview of protein kinase structure and function using cyclic AMP dependent kinase (PKA) as a prototype for this enzyme superfamily. I have demonstrated what we have learned from the overall structural kinome which allows us to compare many protein kinases and also to appreciate how the highly regulated eukaryotic protein kinase has evolved. By comparing many protein kinase structures, we are beginning to elucidate general rules of architecture. In addition, I have attempted to illustrate how PKA is regulated by cAMP and how it is localized to specific macromolecular complexes through scaffold proteins.

[@changyongkang7651]

Thanks DR. Taylor for your great present :)

[@ruxiversi]

Taking my midterm on monday and boy the epinephrine and glycogen phosphorylation and synthesis of glucose is super new though all this energy of g6p and ATP energy...
All in all thanks for posting insulin inhibits opposite of glucagon and epinephrine works

[@thevegandragon4676]

What a superb lecture. Love the history info too. Thank you

[@peters972]

These cascades are quite a theme in biology! Thanks

[@eastofthegreenline3324]

Dr. Taylor, Thank you for posting this well-organized and interesting lecture!

[@iMrYellow]

these lectures are awesome. This whole channel is awesome. Thanks for the content!

[@zakirzak1494]

Beautiful lecture

[@funnychoob]

This video is Great!! Thank you so much for this piece of information!

[@njagikiranga5923]

Great video worth viewing

[@alokpatra86]

Thanks Susan!

[@annakaniauskene2742]

Extremely interesting and enlightening Thank you.

[@jordan-kb9wt]

Awesome, great content and context!

[@zaidzainuddin3744]

great video! zillions of thumbs ups!

[@ginnyli2913]

thanks! I understand this part now.

[@richarddegoeij-dehaas3292]

Great video lecture!!. Performing phosphoproteomics research on biopsy material from cancer patients. Its good to back to the basics for a good understanding.
Especially the historic context is very interesting.
Has someone ever written a book about the history of molecular biology ??

[@farvision]

A search at amazon for "history of molecular biology"
www.amazon.com/s?k=history+of+molecular+biology&ref=nb_sb_noss
picked up the book:
The Eighth Day of Creation: Makers of the Revolution in Biology,
Commemorative Edition Expanded Edition
by Horace Freeland Judson
www.amazon.com/Eighth-Day-Creation-Revolution-Commemorative/dp/0879694785/ref=sr_1_13?keywords=history+of+molecular+biology&qid=1559402613&s=gateway&sr=8-13
It's a bit old (1996) but huge (720 pages) - well known but I have not read it.

[@HML2004]

So much diversity!

[@comeoriginal33]

thank you! i found this very informative and helpful! :)

[@helene02184]

Fantastic, thank you.

[@MichaelHarrisIreland]

Thanks, great video

[@katewinslet7670]

IκBs are a family of related proteins that have an N-terminal regulatory domain, followed by six or more ankyrin repeats and a PEST domain near their C terminus. IκBα is the best-studied and major IκB protein.

[@rodrigomarcondes2000]

Thanks for the class!!!

[@ChrisOfTheInternets]

Very helpful video!

[@CexyNariko]

Thank You so much!!!

[@akrrrs1739]

This is brilliant. Thanks Prof. Taylor

[@carolinegreen4771]

#Kinases transfer the γ-phosphate of ATP to other biological molecules, serving as chemical messengers that make the tight regulation of cell-signaling pathways possible. For example, non-receptor tyrosine kinases are found in the cytoplasm (not membrane-bound) and transfer the γ-phosphate of ATP to tyrosine residues of other proteins, often turning these proteins “on” or “off” in the context of their respective signaling pathway. This notion of “on” or “off” can be useful to holistically conceptualize these pathways but is a gross oversimplification; in reality, many of these enzymes are multi-domain proteins that can exist in multiple conformational states, participate in multiple protein-protein interactions, and experience a gradient of variable activity levels depending on these states. The molecular mechanisms that govern the activity of these kinases is extraordinarily complex, and though much has been discovered in recent years, much remains to be understood, especially for the Tec family of non-receptor tyrosine kinases.

[@electrolyte1000]

Funny she said an average 70 kDa person turns over 40 kg of ATP per day

[@DrMRostami]

Thank you

[@Theodorus5]

80 dalton mass for phosphate group, not 80 kilodaltons :)

[@1swilsons]

I found it great but couldn't focus much because of so much swaying back and forth.

[@tomschneider9460]

4:07 the year should be 1987. See www.ncbi.nlm.nih.gov/pubmed/2434996

[@wuzhannenin42]

Dat comic sans. Love it

[@lizichell2]

this is fabulously interesting = 7 likes