I watched a this for a college presentation and it's nearly completely what i needed to know about it! I eagerly look forward for your new content. Maybe you could cover more techniques in microscopy.
thanks for the clear explaination. Bit off topic but can you share the source of the background music? I think it made the video much nicer when it started around 6:30.
Great question! Thanks for asking! The primary effect temperature has on this type of reaction is changing the binding between the probe and your DNA/RNA. The DNA and probe will not bind at too high of temperatures and too low of temperatures, say below 40C/104F, you have nonspecific binding. This is similar to where I described your probe binding to other RNA/DNA. So you need to find the optimum temperature for your reaction based on your tissue and probe. Does that make sense?
I watched a this for a college presentation and it's nearly completely what i needed to know about it!
I eagerly look forward for your new content.
Maybe you could cover more techniques in microscopy.
Super cool video! Watched it as a resfresher for a job Interview. You did a great job! 😍
Thanks, the way you described this material is so good.
Wonderful explanation, please make a video on whole mount insitu hybridization.
Very clear explanation. Thanks!
Wow! Amazing teacher! thank you for this video :))
Heyyy, that was a great explanation, thank you very much!!
thank you so much for this! i feel like i can pass my exams tomorrow ^^
'gene for people who like hummus' loooool, you're great at explaining things, thank you!
thanks a lot for the video! really informative
thanks for the clear explaination. Bit off topic but can you share the source of the background music? I think it made the video much nicer when it started around 6:30.
Please make more videos about techniques which you use for your research
Thanks, Comrade.
Thank you!
7:25 this was very informative. Also good editing. Thumbs up from a fellow creator 😅
Thanks! I'm really glad you liked it.
YOU SAVED ME
What do you mean by temperature sensitive? More specifically how does the cold affect it?
Great question! Thanks for asking! The primary effect temperature has on this type of reaction is changing the binding between the probe and your DNA/RNA. The DNA and probe will not bind at too high of temperatures and too low of temperatures, say below 40C/104F, you have nonspecific binding. This is similar to where I described your probe binding to other RNA/DNA. So you need to find the optimum temperature for your reaction based on your tissue and probe. Does that make sense?
Thanks for the video. Could flow cytometry be used for the detection of these probes?
¿What should I use in order to quantify the expression of the genes?. Thanks!
There are a few options, I think the best and most widely used tool is qRT-PCR.
Nice video and you have a lot of positive energy, cool!
You could talk about qPCR, and it can be used for quantification, right ?
I am definitely gonna do a video on qPCR. I just wasn't sure when. I'll move it up. Thanks for the nice comment!
I wonder if she has sibling competition issues
Only in video games and school grades and life. 😁
I wish I exhibited the "Super Cool Gene" :(
I mean, you did watch the video....
You're funny
THANK YOU 🤝