Spectrophotometry | Beer-Lambert Law.
Вставка
- Опубліковано 22 лип 2024
- This video is about Spectrophotometry, and discusses in details the Spectrophotometer and Beer-Lambert's law.
In this video I discuss the relationship between the absorbance and the concentration:
- A=epsilon x l x c
- how to determine experimentally the molar absorptivity
- find the concentration of an unknown solution.
You can also watch an experiment using the Beer-Lambert law:
• AP Chemistry Investiga...
Students studying Chemistry at different levels could highly benefit from this video.
I hope you find this video helpful. Please note that the answer at 5:25 is 5.0 x 10^(-3) and not 5.0 x 10^(-2) as it is shown in the video. The preparation for such videos takes lots of time and sometimes small mistakes like this slip easily. Enjoy!
Nice video easy to understand
From India 🤓👌
Double beam
Thanks a lot...very informative video...
i am not too understanding how you get the power
Thank you sir... Your student from Pakistan 👍👍👍💓💓
Woah, everything was so clear. This video is those kinds of videos where you can pause on a certain time and have all the information you need neatly placed with explanations and everything.
Thanks man :D
Really clear and easy to follow explanation. Good job.
Best video on Beer lamberts law...
Thanks
And keep making such videos
Nice informative video. Thank you very much for sharing with us.
You really saved my life. God bless your heart . Thank you so much for sharing your brilliant knowledge 😊 🙏
You explained it really fast and clear. Thank you for this video it will help me in a labolatory!
Thank you so much I was facing problems in this topic but now all is clear
fantastic!! thank you. very clear and perfect pacing.
THANK U VERY MUCH
THE DIAGRAM WITH DESCRIBTION IS VERY USEFUL
Very helpful video. Easy to understand. Thanks.
Really good video! helpful and informative and easy to understand, really good job! thank you so much!:)
راااائع جد..نريد المزيد من الفيديو
Gud lecture sir gathered more information
Amazing explanation. Thanks a lot!
I am watching this in 2018, you are really great Sir, Good explanation, you deserve million subscribers but it's sad nowadays people wants to watch only Crap, youth is destroying their Time & Career.
Thank you very much. It is really appreciated.🙏
Awesome video sir! Thank you!
Wow. Great explanation
Nice explanation Ali Hayek
very good my man,u explain nicely
Good one bro
Wow! I really appreciate it Sir
Excellent lecture
Nice and very useful
dude please make more chemistry videos.... every second of the video had a purpose.
man you do a good job thx
keep up good work ;)
lovely explanation thank you!
The explanation was helpful, thank you
Great explanation! 👍🙏🙏
I have a test about this next week and truth to be told, I did not understood a thing about it, this video helped me to clear it all up so thank you for taking the time to make this. It was really helpful!
+LuciferHasPinkSparklyWings you are more than welcome.
Video was very helpful sir thx a lot
thanks, that's was great it was very helpful
well presented Shukran
thank u so muchh.. such a good video :)
nice work sir
Outstanding
Thank you! It s so easier!
helpful video thaaaaaank you so much
thanks, you are really great sir
Very good video
Thank you very much Sir
tx for uploading very helpful
Thanx bro it really helpful
thank you very much mate!
Love this video
bruh you deserve an emmy for this vid
Time point 5:33, I think there is a mistake: 0.5 and not 5.0
thnq so much sir
JazakALLAH
Thank u so much sir
thank you sir
Gud lecture sir and I have few queries regarding biotechnology
Hello Dr. Hayek,
Thank you very much for this video. The explanation is clear, well-organized, and getting on point, which helps me a lot. I have a few questions. Is there any chemical relation between the concentration of the samples and the incident light emitting or absorbing? I am getting unstable absorbance values with measurements at low concentrations but not at high concentrations. This finding is also beyond the understanding of Absorbance = logarithms of transmission though my values are at 0.1% of T. or could you please enlighten me on the keywords I could find for the articles? Thanks a lot.
Hi there,
Light-matter interaction is a known phenomenon. Sometimes it does not affect the structure of the matter, but some other times light can be aggressive and provides enough energy to break weak bonds. If the absorbance is not stable or keeps changing, it could be that the light is transforming the matter into something that interacts less/more with light. It is a broad topic. I hope this will allow you to start your search. Best of luck.
Is I0 actually the intensity of light as read by detector when a sample of pure solvent (water) is placed in the cuvette?
Nick F yes it is indeed. And this is called the blank and it is used for the background noise correction.
Thank you
Thanks very much for this helpful video.
But I wanna ask about the references, please?
Tq,sir
Super
Please upload beer law for mixture
great video but the answer is 5.0 x 10-3 not 5.0 x 10-2. the video really help me thanks btw.
WATCHDATFREE u know right that calculators of different types gives different value if u don't know then Google it
Yeah I calculated this as well and when I heard it was 5.0 x 10-2 I was confused about what I did wrong xD
i thought i was the only one lol
Good day sir, is the molar coefficient is a constant given per chemical substance or it's something that we can compute too?
I am wondering how am I be able to get the Molar Coefficient of unknown Species and Unknown Concentration, that is present in the water for example lake. This is for spectrophotometer experiment, I can only get the Absorbance and Transmittance but not the
Concentration and Molar Coefficient
In your case you will not be able to determine the concentration or molar extinction coefficient. You will need one of them known to determine the other.
nice
Nice
You are doing excellent job. A little humor will make it perfect.
Can you tell me what software you are using for making these videos.
+BioMania thank you. I use screenflow
At 2:54 % transmittance is denoted as %T and also in last line as A. Didn't get it. Please explain
If you read carefully the last line says that the absorbance (A) can be given in terms of (%T) and the expression is given. I hope this helps.
Hello the video is wonderful.
I can not understand one thing which is when we look the transmittance vs wavelenght graph, why do we observe this shape or when we look the absorption vs wavelenght graph, why do we observe this shape?
Thank you so much
The best way to think about it is that for the absorption spectrum, the higher the intensity of the peak, the more the light is being absorbed by the solution. Now if you think about it in terms of transmittance. The more the light is absorbed, the less of it will transmit. So transmittance measures how much light is going through the solution. So the lower the peak, the lower the percentage of light transmitted. In a simpler way, the transmittance spectrum is the opposite of the absorption spectrum. I hope it is clear now.
Suuuupppppeerrrrr
I am watching from Uzbekistan. How can I contact you?
Go to www.alihayek.com. 👍
more simple calculation of perceent transmittance or OD vaalues in to Percentage will be more useful for basic chemistry students, videos explainigncalculations msy not be available
Sir how find (£)coficiant value
Please reply
This is explained in details by the end of the video. This is the only way to find epsilon. Good luck.
@@AliHayek thank you sir it is very useful video
Answer is 5 x 10^-3
watch this uv visible spectroscopy is explained very briefly...
@
بس لو بالعربي كان فهمت
:)
tx for uploading very helpful