How a Simple UV-visible Spectrophotometer Works

Professor Davis describes a simple example of a double-beam UV-visible spectrophotometer and how it is used to determine the %transmittance of a sample, which can be mathematically converted into absorbance for convenience.

[@ainaanajihahful]

Amazing how I gained so much more from this 6 mins video than from two hours lecture. Thank you so much for the video! Cheers from UW Madison!

[@ChemSurvival]

Thanks for the kind words. It is so exciting to realize that I can reach students across the country and the world this way. I wish you the best of luck in school, but as a Penn State graduate, I think they will take my degree away if I publicly say 'Go Badgers'.... ;-)

[@rainbowlife4646]

ua-cam.com/video/mLlMH8JT-ew/v-deo.html

[@elmelunde]

I rarely give the thumbs up, but think this video deserved it, because it was easy to understand.

[@isragtgonzalez687]

Professor Davis this video is simply excellent, mainly the part where you explain in an easy way the convenience of converting transmittance into absorbance. I definetly check your channel for more useful videos like this one. Great work!!

[@ChemSurvival]

Thanks! This is one of my early offerings on UA-cam, but still among those that make me proudest. I'm so glad that you liked the video. Please pass both the video and my channel along to anyone who might be studying introductory spectroscopy. Cheers!

[@joaquinisraelbolanosgutier4970]

WOW.. what an intresting and easy way to explain the UV Vis Thecnique. Thank you Professor!!

[@PSTWFBo]

Spectroscopy/metry/Photometry! Vis/UV-Vis/IR Spectrophotometer, FTIR, Atomic Fluorescence S., Mass S
ua-cam.com/video/e3qmqHqEns0/v-deo.html

[@govindgiri8849]

Thanx for making UV spectroscopy so much easier.

[@DavidWilson-sm2ym]

There are SO MANY Uv-Vis spectrophotometry videos on UA-cam. I'm been looking them up for an hour. This is the only one without errors and the only one that isn't mind-numbingly boring. Thanks. If I could do animations, I'd do my own. I do wish that you'd animate what is happening in the molecules and atoms though.

[@rainbowlife4646]

ua-cam.com/video/mLlMH8JT-ew/v-deo.html

[@kathleentamayo7874]

Perfect way to explain a concept! Technical terms explained using layman terms and illustrations, then further illustrating concepts/relationships (e.g. using a visibly smaller I to denote a smaller intensity). Will keep this in mind when making presentations. Thanks so much Prof Davis!

[@rainbowlife4646]

ua-cam.com/video/mLlMH8JT-ew/v-deo.html

[@squidpq]

Great video. I really liked your illustrations.
The explanation of why the Beer-Lambert Law is employed was very well done.

[@ChemSurvival]

Thank you for the positive comments. I am truly happy that this video has received such positive reviews! Please pass it on and encourage your friends/classmates/students to subscribe!

[@yohalakshmikumaravelu4482]

Thanks a lot. Perfectly explained along with the images made it much more easier to understand.

[@saralabdoct2755]

That was the best video I've seen on UA-cam on this topic 👌
Thank you sir! Greet job 👏👏👏👏👏

[@6202kamel]

Hi everyone, the home message to take from this video is 'The Beer-Lambert Law states that the higher the concentration of sample in a solution the more light is absorbed by the sample or the higher the absorbance value'.

[@durganshtaneja]

Great video sir! Very helpful. It is almost amazing how the concepts have been simplified and coupled with visuals.

[@KeepRowing247]

it is not almost amazing. it is amazing...

[@peterspencer]

That was excellent. Very easy to understand. Well done sir.

[@ShreeRamJayaRamJayaJayaRam]

explaining why we use absorbance instead of transmittance is good

[@samiramammadova4942]

Thank you very much professor. You explained so clear

[@keantelmeadus2566]

When you do this and you have two different unknown of %T how do you find out the mg%

[@cnarbalc3401]

Amazing explanation, thanks Prof!

[@nimawangchuk9899]

Very much informative piece..thank you professor.

[@pratikjadhav1920]

Very Neat And Clean Explain Sir Thanks For This Video its very much Helpful for those who deeply understanding this Concept😇🙏

[@EminaA]

This was amazing! Thank you so much!

[@nayabferoz2563]

very helpfulll video.... i totally understood ultra visible spectroscopy. ....very nice keep it up sie👍👍👍👍👍👍👍👍

[@sanskaripatrick7191]

Fantastic explanation. Visualization was really helpful.

[@leratohlekelelele2313]

Can you provide a link for the next video lecture after this?

[@sabreenomarful]

loved a lot..got to know very well....thanks professor!!

[@ChemSurvival]

Thank you for the encouraging words. Don't forget to look at the rest of my channel for more instruction in chemistry.

[@razraz4058]

simple and helpful, cheers from Kuwait

[@AquilaLiberum]

How do we get beam attenuation coefficient from these measurements? I am trying to measure water turbidity and am specifically looking for the scattering coefficient but can calculate this is if I can get the total beam attenuation and absorbance coefficients. Also, since I am looking at water as my medium, the light passing through it is just natural sunlight, what wavelength should I use for this? Thanks.

[@kinjabisusa8970]

Thank you for the explanations not really complicated to understand,

[@jonahmunene646]

Thanks Professor Davis..With love from Kenya

[@ChemSurvival]

So glad you liked the video! Best regards.

[@austinnguyen9107]

What happens when you use radio or X Ray light? It seems like it would work if from ur description

[@titi14fan]

thank you professor this has been really helpful

[@jeffmore2793]

zainab hujairi hi miss

[@lokeshgnanasekar]

I really like your presentation Sir! Thank you for the video

[@chikondiliomba642]

You just made it easy for me. thanks alot

[@nurainanajwa4924]

this video really helps a lot, thank you so much!!

[@xochitllee24]

Professor Davis, how does this UV spectrophotometer compare to a UV DOAS multi gas analyzer?

[@duy1609]

Your video helped me very much about UV-VIS, thank you, from Vietnam with love

[@ELICE6]

So easily explained 😱 ❤️ Thank youuu

[@fakhriahrazali2881]

thank you =D easy to understand and really helpful !!!

[@keralacodingacademykca4597]

luved the explanation.well done mate!!!

[@mariellesoldevilla8775]

Thanks Prof!

[@ChemSurvival]

You're welcome!

[@mariellesoldevilla8775]

Tell me how can I ace organic chemistry Sir?😕

[@ChemSurvival]

@@mariellesoldevilla8775 Practice, practice, practice!!! Also, check out my amazing friends Leah of 'Leah4Sci' and Frank from 'Orgo Made Easy'. They both specialize in college-level organic chemistry and they have amazing UA-cam channels.
ua-cam.com/users/Leah4SciTutorials
ua-cam.com/users/OrgoMadeEasy

[@wiwikdwiharyanti2617]

Thank you professor to share this, it very help for my students to understanding.. I used this video to explain them.. thank you very much.

[@rainbowlife4646]

ua-cam.com/video/mLlMH8JT-ew/v-deo.html

[@ananthnm3014]

"plz impruv inglizh" bro its super hard to understand u please use bettr english

[@Pixelaze]

Beautifully explained.

[@esteladapper8892]

Thank you sooo.... freaking much. You are awesome, professor Davis!!!

[@ChemSurvival]

:-)

[@ashutoshbhatia8804]

Thank u professor that's a very satisfied explaination

[@elaafmohsin7165]

really well explained :)

[@slametarifin5369]

How about uv-vis for thin film, powder and solution?

[@AbdulrahmanAlanazi]

Very well explained, thanks!

[@ChemSurvival]

+Abdulrahman Al-anazi Thank you for the feedback! I'm glad you liked the video. There are plenty more on my channel ua-cam.com/users/chemsurvival

[@triras_m1032]

How does the detector converts the absorbance into electric current form

[@madhus.3557]

can you please help me solve this?
Let assume that tryptophan absorbs maximum at 274 nm in benzene. If the tryptophan is transferred from benzene to water where water forms H-bond with excited state tryptophan, what will be the absorbance maxima? The strength of H-bond is 12 KJ/mole.

[@naureenbutt960]

Dear Prof could u plz let me know if we are taking absorbance vs wavelength graph then we have to record absorbance for continuous movement of second slit? As Absorbance vs wavelength graph is very smooth could u plz pour some light on it.I will be really obliged.Tanks for such a nice video

[@mdkamrulhasan7738]

Excellent ..........Professor........Keep it continue. Thanks a lot

[@ChemSurvival]

+Kamrul Hasan Humble thanks, my friend. Lots more coming later this year!

[@s.anandhjesuraj5460]

thank you. it is a easily understandable illustration videos...

[@kskksskkksss]

THANK YOU ....... EASY TO UNDERSTAND

[@GameVoyager17]

nice video. thank you professor .

[@Judyyesulcho]

thanks for this! It was so helpful!

[@rubayettanveer3479]

Great! Keep it up, professor.

[@jayantmharolkar3631]

What is the least count of Spectrometer ?
please answer..

[@Lifeisinruins]

Thank you for explaining this!

[@kledihila831]

professor, you are great! thank you!

[@ChemSurvival]

Glad you liked/shared this! Don't forget to check out my channel at www.UA-cam.com/ChemSurvival and my DVD course at www.chemsurvival.com

[@LazeHeat]

this is gold thanks

[@technovaluesales8394]

Thank you superb explanation

[@vajihehjafari9682]

Thank u so much,it helped me a lot...

[@ahmackalak7553]

Hi Professor Davis! Thank you so much for making this video -- it makes UV Vis easier to understand! I was wondering if I could use your diagram at the beginning of the video in a lab report/cite you as a source?

[@baraskparas9559]

Great presentation!

[@rainbowlife4646]

ua-cam.com/video/mLlMH8JT-ew/v-deo.html

[@usharajbhar7464]

thanks a lot 😊 it's helpful.

[@mohammedarwahhussien9862]

thank you...you made it clear to me

[@salmanahmed5831]

very helpful professor

[@houssemeddinelassoui6823]

Best explanation, thank you.

[@rainbowlife4646]

ua-cam.com/video/mLlMH8JT-ew/v-deo.html

[@johannesmphaka7433]

thank u alot God bless you .....upload more please

[@rahebhafezzadeh8033]

well described, tnx

[@ahmedisse7641]

Great work dr

[@ChemSurvival]

many thanks :-)

[@MdSagar-vb2of]

this video is incredible...

[@chanadrababug3562]

Excellent sir

[@Zabbani]

Thanks 🙏🏻 professor

[@Rekha-qk4dv]

Thank you very much,helped me for my exam tomorrow

[@rainbowlife4646]

ua-cam.com/video/mLlMH8JT-ew/v-deo.html

[@arshadjaved250]

please leave a video over the substitution reactions of various organic compounds, like benzene gives this type of reactions, alkanes, alkenes alkynes give these etc, really hard to understand and memorize them, at least for me. So please do some sort of table, chart or something to memorize them too.

[@ChemSurvival]

I understand your frustration, but I think there is a problem in that you are asking for the lion's share of an entire semester of organic chemistry be distilled into a chart or mnemonic. Many have tried to do this before, some with more success than others. My channel, ua-cam.com/users/chemsurvival has many videos, each of which attempt to touch on a single topic from this category (for example, I have a video on aromatic substitution at ua-cam.com/video/kTINkpnNp3g/v-deo.html ). Unfortunately to cover all possible reactions of all classes of hydrocarbons in one swoop probably isn't going to work out! Thanks for the comment and good luck with your studies.

[@ivana5515]

Pretty clear, Perfect I would say, Thank you

[@rainbowlife4646]

ua-cam.com/video/mLlMH8JT-ew/v-deo.html

[@1987amazonas]

it was pretty helpul thx

[@JaswinderSingh-bh1gr]

Can u plz let me know UV Vis spectrophotometer and UV Vis spectrometer is same or different..?

[@rainbowlife4646]

ua-cam.com/video/mLlMH8JT-ew/v-deo.html

[@ananthnm3014]

same thing

[@marlonaspuria6666]

Thank you , sir.

[@googlesz5162]

Professor, would you mine to give subtitle for your video? Great video by the way, thanks!

[@pug7552]

Great video! Subscribed

[@ChemSurvival]

Thanks!!! I hope you find the rest of the channel helpful as well!

[@toufiqurrahman4029]

thnx .vry heplful indeed.

[@moustafasaadallah41]

very good video

[@shamsabatoolofficial1582]

Amazing 👍🏻

[@mohitdoshi6662]

What is beam splitter? exactly? is that a prism

[@ChemSurvival]

simple beam splitters are just crystals or mirrors that exploit reflection and refraction to direct a portion of an incident beam in different directions. There is a good Wikipedia article explaining for frustrated total internal reflection (crystals) and partial reflection (mirrors) and how these phenomena can split a light beam.
en.wikipedia.org/wiki/Beam_splitter

[@rajashrikarankal5010]

thank u so much sir

[@maxmustermann2707]

Why is it the ratio, what matters? Why not the difference between I and I0? What is the basic law?

[@ananthnm3014]

its just like all detection systems, comparing a smaple against a reference. here they use a ratio, and compare that to a database

[@ankitmaurya3035]

Good one 👍

[@ChemSurvival]

Thanks!

[@halimzaini1796]

Thank a lot.

[@ChemSurvival]

I appreciate the comment. Good luck with your studies!

[@felixmboya5591]

thus very kudos easy to understand

[@PSTWFBo]

Spectroscopy/metry/Photometry! Vis/UV-Vis/IR Spectrophotometer, FTIR, Atomic Fluorescence S., Mass S
ua-cam.com/video/e3qmqHqEns0/v-deo.html

[@jasdeepkaur9356]

thank you so much

[@ByMOCeTrends]

awesome

[@ChemSurvival]

no, your avatar is awesome... my video is just pretty cool B-)

[@sreevidyatanjavuru7049]

thank you sir

[@ChemSurvival]

+Sreekanth Tanjavuru You are welcome!

[@alanax9276]

I started reading about caffeine in coffee and 2 hours later I ended up here.

[@ChemSurvival]

Welcome! 😁

[@brianvandenberg4467]

2:20 good to have baseline data 4 comparisons

[@chorwadibhavesh9498]

thank u sir🙂

[@xyzme1217]

Thank you

[@justicesportsman6020]

Nice video!
However it was a bit slow moving.

[@smclean5504]

Amazing !

[@ChemSurvival]

Thanks, Shadine! Plenty more to see on my channel at www.UA-cam.com/ChemSurvival

[@empace5344]

THANK YOU