A2 Biology - Polymerase chain reaction (PCR) (OCR A Chapter 21.1)
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- Опубліковано 21 тра 2024
- Polymerase chain reaction (PCR) is a crucial technique in genetic manipulation. This "artificial DNA replication" amplifies the number of DNA molecules for further study or processing. This is important as we need a big enough initial stock to ensure we can get good/viable/visible results in the end or perhaps enough to repeat the same experiment again. Here we will discuss the details of the process.
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I have my mock in 2 days and my teacher was so bad with this topic. I've literally been teaching myself half of the whole A level so I hope it goes well. Your videos are a huge help for people like me, and I hope you continue to help as you are. Thank You!
How did it go
thank you that's made it way clearer:) got my aqa a level paper 2 tomorrow v nervous...
Glad to hear that :D All the best tomorrow - you'll perform much better if you relax more :D Rest well and do your best! Thanks for watching :D
Same 😭😭😭
@@MadmaGamer Rest well tonight and just do your best tomorrow! :)
Thank you very much for these omg
Glad you find them helpful :D Thanks for watching!
Thankyou!!
And thank you for watching! :)
thank you :)
No worries! Thanks for watching :)
may u be blessed in this life and the next....thankyou
Thank you so much this made it so much clearer
could you make a video on biotechnology
This is so helpful two days before paper 2!
Haha I'm glad you find it helpful! :D DNA sequencing coming soon!
same here
Thank you, this video was very helpful! Wouldn't mind if it came up on tomorrow's paper now 😂
haha glad it helped! Hope it went well today! Thanks for watching :D
Thank you!
After 3. Is the temperature again raised to 95 degrees frm 72 degrees??
The vid was helpful btw thnx.
Yes it does - this is to repeat the process of denaturing the DNA for the next cycle of PCR. And glad you've found it helpful, thanks for watching! :)
When the free nucleotides pair up, does the primers remain bound? Or do they leave allowing free nucelotides to bind to the part of DNA it has left exposed?
As far as I know the primers stay bound, but I'm not entirely sure... Sorry!
Do you have the videos for chapter 20
Not yet, but I'm aiming to cover all chapters over time so keep checking back :)
ua-cam.com/video/eDbK0cxKKsk/v-deo.html
This is a playlist i found on module 6 it should help ,but Biorach goes into much more detail and depth than these but they are still good to use.
hiya!! your videos are always so helpful to me, thank you for them firstly :)
second - i have a question about the third step (synthesis of DNA) - do the 2 strands join together by DNA polymerase or Taq Polymerase? (dna polymerase is whats mentioned in my text book)
Heya i have the ocr textbook- it says DNA polymerase adds bases to the primer, and the enzyme Taq polymerase is used. I'm pretty sure what this means is that Taq polymerase is a type of DNA polymerase, which is used in PCR because it is able to withstand the extremely high temperatures and doesn't denature. Hope this helps :)
Precisely! :) Taq polyermase *is* DNA polymerase found in the thermophile Thermus aquaticus. It is specifically isolated to use in PCR as it can withstand the high temperatures used in the thermocycler.
I wish you had made these videos sooner, or I had found you sooner:( Biological Diversity tomorrow (Genetics Paper)
Haha still got some more videos to come this week. Good luck with paper 2 and come back for paper 3 next week!
Do you recommend making notes on these videos ?
Yes absolutely! I make videos like how I would make notes for myself if I'm studying :)
suiiiiiiiiiiii
Slight nerves/too fast in speech. But apart from that a really helpful video! :D
Haha yes I do have the tendency to speak quickly - am working on it! Thanks for watching :)