What is HPLC Column ? Check This Video You Will Know

What is HPLC Column ? Do you know what is the Column Inside ?
HPLC stands for High-Performance Liquid Chromatography, which is a technique used to separate, identify and quantify components in a mixture. The column in HPLC is a critical component of the system, and it is where the separation of the mixture takes place.
An HPLC column consists of a stainless-steel tube packed with tiny particles, which can range from a few microns to tens of microns in size. These particles are called the stationary phase, and they interact with the sample molecules to separate them based on their physical and chemical properties.
The stationary phase can be made of different materials, such as silica, polymer, or carbon, and the choice of material depends on the nature of the sample being analyzed. The particles are packed tightly in the column to create a high surface area, which maximizes the interaction between the sample molecules and the stationary phase.
The mobile phase, which is the solvent used to elute the sample, flows through the column and interacts with the stationary phase to separate the sample components. The eluent is pumped through the column at high pressures to ensure a fast and efficient separation.
HPLC columns come in different sizes and shapes, and their properties can be tailored to suit specific analytical needs. The columns can be used for a wide range of applications, such as pharmaceuticals, environmental analysis, food, and beverage testing, and many others.
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