Your lectures are so good that I came to try and answer one pressing question, but stayed to watch about 10 lectures on my day off for entertainment. You should prepare worked examples for each section on a pay site. You have taught the conceptual grounding, now I'm sure people need the application.
I’m confused. You seem to be conflating ionic bonding with hydrogen bonding. I’m curious how exactly pH changes the weaker force of hydrogen bonding. This is a partial charge to do with the distribution of electrons within a molecule rather than it being protonated or not. So how can pH affect that? Many texts simply state that hydrogen bonds are affected by pH without clearly explaining how.
If the concentration of the protonated and non-protonated guanine base is equal at the pH and pKa value of 9.7, wouldn't the percentage of intercellular DNA be 50% at the pH of 9.7? The graph shown looks to have a 50% concentration at about 9.1 pH?
GREAT LECTURE! So I'm trying to understand the basis of Trizol (acid-phenol) RNA isolation... From this lecture: The higher the pKa of a molecule, the more basic the solution has to be in order for the molecule to be half protonated, half deprotonated, correct? The lower the pKa, the more acidic the solution has to be in order for the molecule to be half protonated, half deprotonated, correct? From a Bitesize Article: It says that RNA has a higher pKa than DNA. In Lab: During acid-phenol extraction (low pH), the DNA's phosphate backbone is uncharged. That is because the pKa of DNA is low right? And in that acid-phenol the phosphate backbone is half protonated, half deprotonated which renders to charge neutral. Is all that correct? If you have extra time to answer: Why would RNA's pKa be higher? Is it because of the 2'-OH group or Uracil?
Well for one thing, replication and/or transcription would not be able to take place, which means proteins would not be synthesized, or at least synthesized at a much lower efficiency. Plus, DNA molecules would not be the only ones to experience problems due to drastic changes in pH. All the other biological molecules would also stop functioning effectively, including enzymes. And since almost every process in the body is controlled by enzymes, you can imagine what it would do. In short, you probably wouldn't last too long.
You explained everything in such a logical and easy-to-understand way! Thank you so much!
Your lectures are so good that I came to try and answer one pressing question, but stayed to watch about 10 lectures on my day off for entertainment. You should prepare worked examples for each section on a pay site. You have taught the conceptual grounding, now I'm sure people need the application.
This lecture is absolutely brilliant thank you so much.
If you don't mind me asking where are/were you studying ?
demetronix Thanks, I appreciate that! I studied at NYU.
wonderful explanation
thank you
very well explained...thanks
my life was a lie before your lectures
It was a very nice lecture sir
Perfect
Keep it up 👍
amazing lectures
Thank you!
What about lower ph ?
I’m confused. You seem to be conflating ionic bonding with hydrogen bonding. I’m curious how exactly pH changes the weaker force of hydrogen bonding. This is a partial charge to do with the distribution of electrons within a molecule rather than it being protonated or not. So how can pH affect that? Many texts simply state that hydrogen bonds are affected by pH without clearly explaining how.
suggest u to make chapterwiswe videos for biochemistry covering lippincot book
If the concentration of the protonated and non-protonated guanine base is equal at the pH and pKa value of 9.7, wouldn't the percentage of intercellular DNA be 50% at the pH of 9.7? The graph shown looks to have a 50% concentration at about 9.1 pH?
Спасибо за ваши лекции.
thanks
does anyone know where this guy went? he is excellent
GREAT LECTURE!
So I'm trying to understand the basis of Trizol (acid-phenol) RNA isolation...
From this lecture: The higher the pKa of a molecule, the more basic the solution has to be in order for the molecule to be half protonated, half deprotonated, correct? The lower the pKa, the more acidic the solution has to be in order for the molecule to be half protonated, half deprotonated, correct?
From a Bitesize Article: It says that RNA has a higher pKa than DNA.
In Lab: During acid-phenol extraction (low pH), the DNA's phosphate backbone is uncharged. That is because the pKa of DNA is low right? And in that acid-phenol the phosphate backbone is half protonated, half deprotonated which renders to charge neutral. Is all that correct?
If you have extra time to answer: Why would RNA's pKa be higher? Is it because of the 2'-OH group or Uracil?
amazing!
Pleas can you give me the references that you have used
does anyone else watch these videos at 2x speed lol
What does it mean for your body when the double helix separates, in the case that you have a higher pH level?
Well for one thing, replication and/or transcription would not be able to take place, which means proteins would not be synthesized, or at least synthesized at a much lower efficiency. Plus, DNA molecules would not be the only ones to experience problems due to drastic changes in pH. All the other biological molecules would also stop functioning effectively, including enzymes. And since almost every process in the body is controlled by enzymes, you can imagine what it would do. In short, you probably wouldn't last too long.
what happens @ acidic pH