If your sequence contains more than 14 nucleotides, the equation is Tm=64.9+41[(G+C - 16.4) / total in sequence]. If your sequence contains less than 14 nucleotides, the equation is Tm=2(A+T)+4(G+C). In the equation in your video, the answer should be 46.77. The equation would look like Tm=64.9+41[(8-16.4)/19] = 46.77
Thank you ❣️
If your sequence contains more than 14 nucleotides, the equation is Tm=64.9+41[(G+C - 16.4) / total in sequence]. If your sequence contains less than 14 nucleotides, the equation is Tm=2(A+T)+4(G+C). In the equation in your video, the answer should be 46.77. The equation would look like Tm=64.9+41[(8-16.4)/19] = 46.77
Ok thank you for your comment. We will surely look into this.
Mam if there is more than 14 nucleotide. The formula is different
Very easy process. Thank you ma'am. Looking forward for your upcoming video.
Thanks a lot 😊
very easy to remember indeed
Thanks a lot. Keep supporting BIOGUIDE
This is very useful 👍
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