Just a suggestion, instead of "scooping up" the old piece (which carried contamination) I would reccomend cutting, precisely, a small piece of the leading edge of the mycelium on clean agar and transferring that, the smaller the better...
I have just tried something and it looks like it worked. Instead of moving mycelium out, I poured a little hydrogen peroxide in the plate just up too the mycelium not over it. Let it sit a few minutes then poured it out. What also poured out was the bacteria. Did a 2nd pour of peroxide to get any remnants. It’s been 4 days and the plates look clean. And the mycelium is still growing.
Just a suggestion, instead of "scooping up" the old piece (which carried contamination) I would reccomend cutting, precisely, a small piece of the leading edge of the mycelium on clean agar and transferring that, the smaller the better...
very good comment. my cuts were sloppy for sure.
I have just tried something and it looks like it worked. Instead of moving mycelium out, I poured a little hydrogen peroxide in the plate just up too the mycelium not over it. Let it sit a few minutes then poured it out. What also poured out was the bacteria. Did a 2nd pour of peroxide to get any remnants. It’s been 4 days and the plates look clean. And the mycelium is still growing.
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I recon the flame torch was blowing the mould around, id point it the other way if i try this