Thanks! In the case of the gut content of fish larvae, could you suggest or make a video on how to isolate DNA? Because the size of the sample (larvae) itself is microscopic
Hi! Nice video. My question is: are you not concerned that the solution you use might get contaminated when you take the solution directly from the bottle? In other protocols, an appropriate amount of solution from the bottle is transferred into a smaller bottle. The amount of the solution will then be extracted from the smaller bottle. This can reduce the possible contamination of the whole solution (big bottles).
Yes we are concerned where their is the requirement to think about the contamination part, but here we work with bulk quantities, like in liters and kilos. When the precaution is needed to be taken we are always alert. Only those solutions which are not affected are handled in a rough manner, otherwise each and every step is monitored and SOP are followed.
Excellent explanation Ma'am please make a video on isolation of a specific cell from tissue.. Like i want to isolate only mast cell from connective tissue which approach i can apply
I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"
New research k liye aap recent research papers read karo, we wanted to demonstrate dna isolation protocol in this video which we did and not new research in biotechnology.
Well initiative to explain this practical experiments by youtube it will be very helpful to students of biotech
Thank you professor 😊
Good initiative by PDKV Biotech Products Good wishes for more such creation will certainly helpful to others
Loved the explanation and practical demonstration!
Thank you
Exceptionally good. Best wishes.
Many thanks!
Well explained guys .. appreciate ur efforts...
Thank you Mayur 😊
This is actually helping before examination
👍
This is an awesome presentation video.
i have a question, what should one do if the pellet does not form after precipitation?
Mam we proud u are Vncabian's✨❤
Thank you so much Omprakash 😊 #VNCAB
Excellent jop dear Friends
Thank you
Thanks for the movie. Everything I did understood, but not the first dissolver after homogenization. What did you used? "Protein SK"?
It's a protinase k used to digest protein and remove contamination from preparations of nucleic acid
Excellent
Thanks! In the case of the gut content of fish larvae, could you suggest or make a video on how to isolate DNA? Because the size of the sample (larvae) itself is microscopic
Very nice
Very nice .... Keep it up 👍👍
Nice
Hi! Nice video.
My question is: are you not concerned that the solution you use might get contaminated when you take the solution directly from the bottle? In other protocols, an appropriate amount of solution from the bottle is transferred into a smaller bottle. The amount of the solution will then be extracted from the smaller bottle. This can reduce the possible contamination of the whole solution (big bottles).
Yes we are concerned where their is the requirement to think about the contamination part, but here we work with bulk quantities, like in liters and kilos. When the precaution is needed to be taken we are always alert. Only those solutions which are not affected are handled in a rough manner, otherwise each and every step is monitored and SOP are followed.
@@ampliconsofbiotech Thank you for this information.
Love the explanation 👍
Thank you 👍🏻😊
Keep Going bro.. 🤝
Ek no. Guys👍👍
Thank you
Excellent explanation
Ma'am please make a video on isolation of a specific cell from tissue..
Like i want to isolate only mast cell from connective tissue which approach i can apply
👍👍
Can you send me the research article details that you referred please 🙏 . Urgently
What is composition of lysis buffer that you used..??
We have mentioned the components in the description
Please provide the Molar concentration of EDTA and tris-CL added and percentage of SDS added in lysis buffer.
Superb 😍
Thank you
👌👌👌
I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"
I will have to Check
What homogenizer are you guys using?
We are unaware of the machine brand sorry.
Share your lysis buffer recipe and if you think it can't be public, you can ask for my email. Thankyou
Yes sure, share your email address here
Ye Bhushanu....
😅
👌
Fish Dna extracted Nice
Mam most humbly I want to know that you are students of biotechnology?If yes then from which college.
Not college students, working professionals in ICAR
Mam kuch asa batai hum bhi biotechnology ka student hai kuch new batai
New research k liye aap recent research papers read karo, we wanted to demonstrate dna isolation protocol in this video which we did and not new research in biotechnology.
Practical ke written note mil sakte h kya
Aisa kuch printed material nahi hai written notes k liye. We refer to research paper's and articles. But we can provide if you want.
It should not be protein sk but proteinase k i think so
Yes dear it's proteinase k only, those are auto generated subtitles by UA-cam which sounds like protein sk
Nice
👍👍