Column chromatography

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  • Опубліковано 22 сер 2024
  • Column chromatography is a technique in which separation is carried out in the column. The column is filled with a stationary phase. The sample is loaded with the mobile phase on the top of the column. Once the sample is loaded the mobile phase is continuously passed to cause separation. The mobile phase moves down the column because of gravity. Separation of molecules occurs due to their differences in interaction with mobile phase and the stationary phase.

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