Histological staining: hematoxylin & eosin

The most popular and one of the principal stains in histology is hematoxylin and eosin stain. It gives us an overview of the tissue and its structure. Hematoxylin binds with basophilic structures - for example DNA and RNA. So we can observe nuclei stained in blue or purple color. Eosin binds to acidophilic substances such as positively charged amino acid side chains. So as the result cytoplasm is pink or orange. All samples in laboratory are stained with H&E. There are several different types of hematoxylins and eosins used in histology which will give us different results.
In this video you will see, how we stain slides with different types of hematoxylins and eosins. Finally, we will compare the results.
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[@user-go5ng2cr8b]

great! Thank you very much

[@GonzalesDalidien]

Hello, thank you for the video
Can you please show us how to filter the stain?? We used to get ( impurities, dirty particles, bacterial or fungal like elements) in our hematoxylin stain after a while of using , can you please show the solution and the method of filtration ?

[@brkhra.k.a.legatus3183]

It is a very help video. Thank you so much for your efforts.

[@mohamedroshdy882]

Thanks you very much for your show

[@unsaltedegg]

Thank you! This helps me a lot!

[@Valentina-he2uu]

Bella zi, mi hai salvato l’esame di istologia 💪🏻

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[@marcelsincere9933]

@Brennan Conner Instablaster :)

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[@marcelsincere9933]

@Brennan Conner Glad I could help =)

[@nooriafghan7636]

Please can you tell us combination of hematoxilin and acitic acid mean's how much acitic acid can add to 500ml hematoxilin

[@abdifatahfarahhamud6426]

Good presenter, i preferred how This cute girl presented with Dignity ! 👏👏👏

[@mirraju3744]

Thanks

[@MohsinAli-cn5wr]

@BioVitrum, What will be the procedure for detecting the microneedle effected area with H and E staining?

[@kwabenaadu-twum50]

How would you determine the end point of fixation?

[@muhammadmoazzam2662]

Hi, how to make our sample stable on the slide?

[@arshaankhan2564]

Explain is good 🙏

[@begum2079]

Does the time we use for hematoxylin and eozin make big changes?

[@NicksLocker]

Yes, it does

[@goldenheart1667]

Thanks a lot

[@ernestdutch3586]

Helped me a great deal

[@sachchajay5506]

Could you please explain how xyelen make the smear transparent ?

[@russ9445]

because xylene is a fluid with a property that has high index of refraction giving it a transparent look hence the name clearing, hope this helps :)

[@russ9445]

THANK YOUUUUQ

[@jowairiady1931]

Why do we need to dehydrate again after staining?

[@lajoswinkler]

Because it needs to be cleared and embedded into a mountant with similar refractive index as the glass slide and that mountant is nonpolar.

[@jowairiady1931]

@@lajoswinkler this is quite late, but thank you for answering 😊💛

[@militaryenthusiast1929]

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[@jowairiady1931]

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[@devarapallisubbareddy1139]

Lab technician work super

[@tr4or]

❤❤

[@_abubakraljelly7343]

I like you very match 😍😍😍

[@fairy-pc6xo]

А на русском слабо????

[@user-wz9yn4de3f]

Спасибо 👍