Hi Doctor Thx u for making biochem so easy You are a life saver Could you please make a video on enzymes topic (enzyme kinetics n inhibition) I'm a Fmge aspirant
Rothera's test is used to detect the presence of ketone bodies in a solution (eg urine). it utilizes sodium nitroprusside, liquid ammonia and ammonium sulphate. ammonium sulphate is used to concentrate the ketone bodies to the center of the solution. the other 2 constituents react with ketone bodies to form pink coloured polymerized compound. Ammonium ion is used to precipitate the proteins that may be present.. Because proteins can give a positive result to Rothera's test as well.. And it make solution Heavier than liquid Ammonia, so Ammonia may be remain on top of solution ,so purple ring is formed. hope it helps
Hi Doctor
Thx u for making biochem so easy
You are a life saver
Could you please make a video on enzymes topic (enzyme kinetics n inhibition)
I'm a Fmge aspirant
Hey naveen. Thanks for the kind words. Yes will make video series on enzymes once mol bio gets over.
@@Biochemistrybasics Thx u Doctor
Must video sir ji
🙏😊
Sir
Why urine sample is saturated with ammonium sulphate?
Any specific reason ?
Rothera's test is used to detect the presence of ketone bodies in a solution (eg urine). it utilizes sodium nitroprusside, liquid ammonia and ammonium sulphate. ammonium sulphate is used to concentrate the ketone bodies to the center of the solution. the other 2 constituents react with ketone bodies to form pink coloured polymerized compound. Ammonium ion is used to precipitate the proteins that may be present.. Because proteins can give a positive result to Rothera's test as well.. And it make solution Heavier than liquid Ammonia, so Ammonia may be remain on top of solution ,so purple ring is formed.
hope it helps
@@Biochemistrybasics it really really help
@@Biochemistrybasics thankyou so much sir
Amazing explainnn thanks doctorrr
Most welcome dear
***What is the name of the purple coloured complex?***
By the way nice video👍🏻
Thanks Abhijnyan. As far as I know there is no any particular name of that ring.
Why is strong ammonia slowly adddddd pls give answerrrrr
Sodium iso Nitro Ferro Penta cyano compound